Separation of native and denatured fractions from partially denatured pneumococcal DNA

Roger, Muriel; Beckmann, Charles O.; Hotchkiss, Rollin D.

doi:10.1016/s0022-2836(66)80083-9

Cited by 21 publications

(8 citation statements)

References 12 publications

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“…Recovery (as a percentage of input DNA): total, 82.3; L, 40.0; H, 38.4. Pooled L tubes (21)(22)(23)(24)(25)(26)(27) and H (tubes 32-37) were self-annealed (68 C, 4 hr) at the salt concentration of the eluates and dialyzed against 0.05 M sodium phosphate (NaP), pH 6.7. (B) HA profiles of self-annealed pooled L and H fraction.…”

Section: Resultsmentioning

confidence: 99%

“…The question that arises relevant to strand resolution by MAK chromatography is whether fragmentation occurs at unique break points or whether the sites of breakage are randomly distributed between the strands. Roger et al (25) concluded from extensive sedimentation boundary measurements of unfractionated and MAK-fractionated denature pneumococcus DNA that chromosomal breakage occurs during preparation at predetermined regularly spaced intervals (24,25). Gabor and Hotchkiss (9, 10) gave further evidence for homogeneity in pneumococcal DNA preparations by demonstrating differences in the rate of phenotypic expression of different markers when hybrid duplex free from its mirror image is used.…”

Section: Discussionmentioning

confidence: 99%

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Chromatographically Fractionated Complementary Strands of Bacillus subtilis Deoxyribonucleic Acid: Biological Properties

Rudner

Remeza

1973

J Bacteriol

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Biological, physical, and chromatographic properties of methylated albuminkieselguhr (MAK)-fractionated complementary strands, designated as light (L) and heavy (H), of Bacillus subtilis deoxyribonucleic acid (DNA) are presented. The pattern of transforming activity along the MAK elution profile of alkilidenatured DNA shows that the residually active molecules selectively fractionated ahead of the L strand fraction, whereas the most active self-annealed molecules fractionated preferentially at the trailing end of the H strand fraction. The restoration rate of transforming activity in the late-eluting H molecules was rapid and independent of concentration during the annealing reaction. The data suggest that the self-annealing activity in the H strand is due in part to the formation of intrastrand secondary structures. Hydroxyapatite chromatography of self-annealed L and H strands yielded a major fraction (I) of highly purified strand preparations devoid of transforming activity and hypochromicity, and a minor "nativelike" fraction (II). Sedimentation velocity measurements show that, in addition to the mutual complementary nature of the L and H fractions, they differ in molecular size and possibly configuration.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Chromatographically Fractionated Complementary Strands of Bacillus subtilis Deoxyribonucleic Acid: Biological Properties

Rudner

Remeza

1973

J Bacteriol

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“…Genetic markers are clearly heterogenous in their physical properties. They have markedly different buoyant densities (14), thermal inactivation temperatures (14,20,24), and sensitiviies to UV (15,18,19,20,27). It has generally been concluded that the UV sensitivity of a marker in DNA irradiated in vitro is correlated with the percentage of adenine plus thymine in the neighborhood of the markers (15,18,19,20,22,27).…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Repair of Ultraviolet Damage by Competent and Noncompetent Cells of Bacillus subtilis

Hadden

Billen

1973

J Bacteriol

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The repair of ultraviolet (UV) damage in Bacillus subtilis W23T − has been studied by transformation with deoxyribonucleic acid (DNA) extracted from irradiated cells before and after repair. The extent of repair of genetic markers by donor cells after low or moderate doses of UV was found to be related only to the initial degree of inactivation. After a very high dose, further inactivation occurred, also in proportion to initial damage. In addition, the competent recipient cells were shown to repair approximately 75% of the damage in transforming DNA. The sensitivities of markers irradiated either in vivo or in vitro appeared to be related to map position, the more proximal markers showing a greater resistance to UV inactivation.

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“…Such trailing has been observed during elution of homogeneous native DNA's as well as mixtures of native and denatured DNA. [2][3][4] Recycling of the fractions obtained under each of the two peaks should show to what extent this is true for the resolution of complementary strands and could also provide fractions with improved resolution. Both objectives have been accomplished.…”

Section: Biochemistry: A! Rogermentioning

confidence: 99%

Chromatographic resolution of complementary strands of denatured Pneumococcal DNA.

Roger¹

1968

Proc. Natl. Acad. Sci. U.S.A.

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Separation of native and denatured fractions from partially denatured pneumococcal DNA

Cited by 21 publications

References 12 publications

Chromatographically Fractionated Complementary Strands of Bacillus subtilis Deoxyribonucleic Acid: Biological Properties

Chromatographically Fractionated Complementary Strands of Bacillus subtilis Deoxyribonucleic Acid: Biological Properties

Genetic Analysis of Repair of Ultraviolet Damage by Competent and Noncompetent Cells of Bacillus subtilis

Chromatographic resolution of complementary strands of denatured Pneumococcal DNA.

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