Separation of nucleic acid precursors on an amphoteric surfactant modified monolith using combined eluent flow, pH and concentration gradient

Abstract

An RP monolithic column coated with an amphoteric carboxybetaine type surfactant has been used with a combined triple eluent concentration, pH and flow gradient ion chromatography technique for the simultaneous separation of up to 18 nucleotides, nucleosides and nucleobases. The separation of up to eight precursors on a 1 cm long monolithic microcolumn using the combined gradient approach is also shown. The method was applied to the separation of the above nucleic acid precursors in perchloric acid extracts of yeastolates samples.

Silica‐based zwitterionic monolithic stationary phase for separation of neutral and ionized solutes using pressurized CEC

“…Therefore, nucleotides are excellent model solutes to study the interplay of ionic, polar characters for solute retention on the zwitterionic monolith. Separation of nucleotides on an (N-dodecyl-N,N-dimethylammonio)undecanoate-coated zwitterionic monolithic column with a combined triple gradient ion chromatography technique has recently been reported by Nesterenko et al [48]. In this study, good separation of five nucleotides was obtained by the HI-pCEC mode with 75% v/v ACN and pH 6.5 in 10 mM TEAP as the mobile phase.…”

Determination of adenine based on the fluorescence recovery of the L-Tryptophan–Cu2+ complex

“…A number of analytical methods based on electrochemistry [8][9][10][11], chromatography [12][13][14], colorimetric method [15], fluorescence [16], resonance Rayleigh scattering [17], and chemiluminescence [18,19] have been proposed to provide fast and sensitive means for determination of adenine.…”