Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

Ayano, Eri; Sakamoto, Chiaki; Kanazawa, Hideko; Kikuchi, Akihiko; Okano, Teruo

doi:10.2116/analsci.22.539

Cited by 39 publications

(22 citation statements)

References 29 publications

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“…Some types of functional surfaces were created by a surface modification method, including terminally, [18][19][20][21][22][23][24][25][26] cross-linked, [26][27][28][29] and created polymer brush. [29][30][31][32][33][34][35][36][37][38][39] For modification involving one end of a polymer to a silica surface, the synthesis of PNIPAAm was carried out by radical polymerization using 3-mercaptopropionic acid (MPA) as a chain-transfer agent.…”

Section: Synthesis Of Polymer-modified Packing Materials 25mentioning

confidence: 99%

“…Additionally, a polymer layer formed on silica beads showed resistance to an alkaline solution. 26 Recently, controlled free-radical polymerization techniques have been utilized to synthesize polymers. Nagase [33][34][35][36] et al reported that a "grafting from" method for PNIPAAm involves high-density PNIPAAm brushes on silica-bead surfaces using a surface-immobilized atom transfer radical polymerization (ATRP) initiator.…”

Section: Synthesis Of Polymer-modified Packing Materials 25mentioning

confidence: 99%

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Temperature-responsive Smart Packing Materials Utilizing Multi-functional Polymers

Ayano

Kanazawa

2014

ANAL. SCI.

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Section: Synthesis Of Polymer-modified Packing Materials 25mentioning

confidence: 99%

Section: Synthesis Of Polymer-modified Packing Materials 25mentioning

confidence: 99%

Temperature-responsive Smart Packing Materials Utilizing Multi-functional Polymers

Ayano

Kanazawa

2014

ANAL. SCI.

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“…These studies typically combine changes in pH and temperature to modify surface properties. [146][147][148][149] This approach may be applied to chromatography, microfluidic networks and biosensors, where it will offer an advanced level of control.…”

Section: à2mentioning

confidence: 99%

Intelligent Control of Surface Hydrophobicity

et al. 2007

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Switchable surfaces are highly useful materials with surface properties that change in response to external stimuli. These surfaces can be employed in both research and industrial applications, where the ability to actively control surface properties can be used to develop smart materials and intelligent surfaces. Herein, we review a range of surfaces in which hydrophobicity can be controlled. We present the principal ideas of surface switching, discuss recent developments, explore experimental issues and examine factors that influence surface switching, including the nature of the stimuli, the underlying material, the morphology of the surface and the surrounding environment. We have categorised switchable surfaces according to the stimuli that trigger changes in surface hydrophobicity. These are electrically, electrochemically, thermally, mechanically, photo- and environmentally inducible surfaces. In addition, we review the use of chemical reactions to modify the properties of switchable surfaces and produce changes in the molecular structure and nanoscale features of the surface.

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“…This alteration of the PIPAAm-modified intelligent surface can be used to modulate its interactions with solutes in the chromatographic separation [15][16][17][18][19][20] and with cultured cells [21][22][23]. PIPAAm-grafted chromatographic matrices allow us to regulate the retention of steroids [15,16], polypeptides and proteins [17,18], and nucleotides [19,20] by changing the temperature under the aqueous condition. This is an alternative to reverse-phase chromatography, where separation is controlled by regulating the interaction between octadecyl stationary phase surfaces and solute…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Kobayashi¹,

Okano²

2010

Science and Technology of Advanced Materials

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This article reviews the properties and characterization of an intelligent thermoresponsive surface, which is a key technology for cell sheet-based tissue engineering. Intelligent thermoresponsive surfaces grafted with poly(N-isopropylacrylamide) exhibit hydrophilic/hydrophobic alteration in response to temperature change. Cultured cells are harvested on thermoresponsive cell culture dishes by decreasing the temperature without the use of digestive enzymes or chelating agents. Our group has developed cell sheet-based tissue engineering for therapeutic uses with single layer or multilayered cell sheets, which were recovered from the thermoresponsive cell culture dish. Using surface derivation techniques, we developed a new generation of thermoresponsive cell culture dishes to improve culture conditions. We also designed a new methodology for constructing well-defined organs using microfabrication techniques.

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Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

Cited by 39 publications

References 29 publications

Temperature-responsive Smart Packing Materials Utilizing Multi-functional Polymers

Temperature-responsive Smart Packing Materials Utilizing Multi-functional Polymers

Intelligent Control of Surface Hydrophobicity

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

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