Separation of phenylacetic acid, 6‐aminopenicillanic acid and penicillin G with electrodialysis under constant current

Abstract

Abstract:The separation behavior of phenylacetic acid (PAA), 6-aminopenicillanic acid (6-APA) and penicillin-G (Pen-G) with electrodialysis under constant current was studied. The effects of ionic concentrations and current density on their separation behavior were investigated. The sorption of PAA, 6-APA and Pen-G in the anion exchange membrane, and the variations of applied voltage and current efficiencies with time during electrodialysis were also examined. It was found that the molecular size and the affinity toward the anion exchange membrane played a key role for the electrodialysis of PAA, 6-APA and Pen-G. Phenylacetic acid had the lowest affinity toward the membrane but the fastest transport rate. Penicillin-G had the highest affinity toward the membrane but the slowest transport rate. With the increase of Pen-G concentration, the concentration polarization of Pen-G in the vicinity of anion exchange membrane became very serious, which severely retarded the transport of PAA and 6-APA. Although the increase of current density accelerated the transport of PAA, 6-APA and Pen-G, more serious concentration polarization of Pen-G occurred and the separation ratio of PAA to 6-APA decreased.

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

“…Chen et al [49][50][51] and Huang et al [52] used electrodialysis separation of the reaction components of penicillin G hydrolysis in a membrane reactor-separator for enhancing the efficiency of the process. Ishimura and Suga [47] used this technique to remove inhibitory phenylacetic acid in a membrane reactor-separator, reducing to 65% the necessary time to reach 96% yield.…”

“…Many studies [46][47][48][49][50][51][52][53][54] address the reaction components separation or an inhibitory component removal by an electrodialysis process using usually soluble enzymes. Electrodialysis can be defined as a type of technology that arranges ion-exchange membranes (cation-selective, anion-selective and/or bipolar membranes) alternately in a direct current field [55][56][57][58][59].…”

Knowledge‐Based Conceptual Synthesis of Industrial‐Scale Downstream Processes for Biochemical Products

“…Four out of 16 suggested alternatives are validated as candidates for the second penicillin V downstream technology based on literature: chemical precipitation 19, 21, ion exchange 22, organic extraction 20, 23, and aqueous two‐phase extraction 24. Further alternatives exist that either show limitations as in the case of protein precipitation with organic solvents, electrodialysis 25, extraction with supercritical CO 2 26, and chromatography 27, or would require an experimental proof‐of‐concept, e.g., in the case of adsorption, electrical separations, and centrifugal partition chromatography.…”

The Application of Electrodialysis to Desalting an Amino Acid Solution

“…Electrodialysis (1-7) is a useful and well-known method for desalting solutions with organic products (8)(9)(10)(11)(12)(13)(14)(15)(16). On application of an electrical field to a solution where one or several electrolytes are present as cations and anions, the cations migrate toward the cathode and the anions migrate toward the anode.…”