Separation of Polyunsaturated Fatty Acid Methyl Esters by Gas Chromatography

The Fatty Acid Patterns of Plasma Lipids During Alimentary Lipemia*

“…Separations were carried out using two types of stationary phase: a) Apiezon L vacuum stopcock grease at 1970 C. (14); and b) a polymer of ethylene glycol and adipic acid at 175°C. (15)(16)(17). In the earlier work, the gasdensity balance (18) was used as the vapor detector, total loads of ester separated being approximately 3 mg. At this level of sensitivity large samples of plasma were required (50 to 75 ml.)…”

Gas Chromatographic Analysis of the Fatty Acid Composition of the Plasma Lipids in Normal and Diabetic Subjects*

“…For a good separation of substances with a high molecular weight it was necessary to increase the temperature of the injection block to about 30 to 40°C above the temperature of the column. The mixtures of the methyl esters of the fatty acids were analyzed on two stationary phases, viz., silicone (Dow-Corning high vacuum grease) and Reoplex 400 (Geigy) (16,17). The silicone column packing consisted of 30 parts by weight of stationary phase to 100 parts of solid support (Sil-OCel, 50 to 75 mesh) which had been pretreated with acid and alkali and carefully washed with water.…”

Recent advances in the analytical chemistry of fatty acids and derivatives

“…The rapid separation of the cis and transisomersof fatty acids using packed columns has long been an intriguing prob!e,~,.~t can be dor',e with cap~!ary columns, bat this is a time-consuming analysis. The introduction of the cyano-propyI silicone liquid phases was a great advance in the separation of the geometrical isomers of fatty acids (5,6) and also of some of the derivatives (7). These highly polar liquid phases are variously known as Silar 5C, Silar 10C, Silar 9CP, SP 2340 and OV-275.…”