Separation of six lipid classes on one thin-layer chromatogram

Hojnacki, Jerome L.; Smith, Samuel C.

doi:10.1016/s0021-9673(00)92542-1

Cited by 48 publications

(12 citation statements)

References 5 publications

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“…Liver tissues were extracted according to the technique of Folch et al (1957), serum was extracted as described by Chedid et al (1972) and the lipids were separated and identified by thin layer chromatography according to the method of Hojnacki and Smith (1974).…”

Section: Lipid Extraction and Chromatographymentioning

confidence: 99%

Biochemical changes associated with fatty liver in geese

et al. 1984

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SUMMARYStudying biochemical changes in the blood and liver of geese during cramming showed significant increases in the liver enzymes: malic dehydrogenase (MDH), lactic dehydrogenase (LDH), aspartate aminotransferase (AST) and malic enzyme (ME), and a decrease in alkaline phosphatase (ALP). No significant changes were seen in the activity of isocitric dehydrogenase (ICDH), and glucose 6 phosphate dehydrogenase (G6PDH). There were significant increases in serum ME, ICDH, LDH, MDH, AST, acid phosphatase (ACP), sorbitol dehydrogenase (SDH), and total lipids and decreases in serum ALP, albumin and the haemocrit. No significant changes were seen in the activity of cholinesterase, glucose, total proteins, globulins and inorganic phosphorus. There were good correlations between liver size and the change of some of the biochemical parameters studied, which may serve as markers for the presence and degree of liver fattening. There were differences between families of gray and white geese and concentrations and activities of the blood constituents paralleled the degree of liver fattening. The possibility of using these parameters as genetic markers is discussed. No correlations were found between the liver and serum biochemical parameters. The effect of transporting the geese from the farm to the slaughter house on the levels of the blood constituents is described.

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Section: Lipid Extraction and Chromatographymentioning

confidence: 99%

Biochemical changes associated with fatty liver in geese

et al. 1984

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“…and esterified CH at a concentration of 1 gg/Ml were then fractionated by thin-layer chromatography on Whatman K6 silica gel an.alytical thin-layer chromatography plates (20 X 20 cm, 250 ,um; Whatman, Inc., Chemical Separation Div., Clifton, NJ) with the use of a two-solvent system as described by Hojnacki and Smith (33) and modified by Hajjar et al (31). CH and CE were subsequently quantitated by microfluorometric analysis (32 was equally enhanced from base-line levels by PGI2, regardless of the presence or absence of MIX, a phosphodiesterase inhibitor (Table I).…”

Section: Prostacyclin Modulates Cholesteryl Ester Hydrolysis 481mentioning

confidence: 99%

“…Sodium palmitate and sodium eicosatrienoate were used as controls. [30][31][32][33][34][35][36][37][38][39][40] ,uM sodium arachidonate significantly enhanced (P < 0.05) both PG production and ACEH activity. When SMC were challenged with 10, 20, 30, and 40 gtM sodium arachidonate, they produced 3.7, 4.1, 4.8, and 5.8 nM 6-keto-PGFI,a, respectively.…”

Section: Prostacyclin Modulates Cholesteryl Ester Hydrolysis 481mentioning

confidence: 99%

Prostacyclin modulates cholesteryl ester hydrolytic activity by its effect on cyclic adenosine monophosphate in rabbit aortic smooth muscle cells.

Hajjar¹,

Weksler²,

Falcone³

et al. 1982

J. Clin. Invest.

164

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A B S T R A C T We tested the hypothesis that prostacyclin (PGI2), 6-keto-prostaglandinF,,,(6-keto-PGF,a), and several E series prostaglandins (PG) may affect the activity of cholesteryl ester (CE) hydrolase since our previous experiments indicated that smooth muscle cells (SMC) in neointima of injured rabbit aorta (a) acquire the capacity to produce PGI2 and (b) have increased lysosomal CE hydrolytic (acid cholesteryl ester hydrolase [ACEH])activity. Using cultured SMC from rabbit thoracic aorta, we demonstrated that PGI2, 6-keto-PGF1,, and 6-keto-PGE, enhanced ACEH activity fourfold. No significant effects on ACEH activity were observed with PGE1 or PGE2. Preincubation of SMC with an inhibitor of adenylate cyclase activity (dideoxyadenosine) abolished the effect of these PG on CE hydrolytic activity. Addition of dibutyryl cAMP to these SMC significantly increased ACEH activity.Although concentrations of PGI2 used significantly increased cAMP levels, proliferation of these SMC was not observed.In related experiments, we determined if the addition of PGI2, 6-keto-PGF,a, or 6-keto-PGE1 to cultured aortic SMC would enhance the egress of unesterified cholesterol and CE from these SMC. A significant loss of total cholesterol. from PG-treated SMC Parts of this study were presented at a meeting of the Federation of

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“…Samples were resuspended in 100-200 ,1 chloroform/methanol (2:1, vol/vol). The extract and a lipid standard containing CHOL and CE (1 Ag/yl) were fractionated separately by thin layer chromatography (19). A two-solvent system was used to separate CHOL and CE (20).…”

Section: Introductionmentioning

confidence: 99%