No abstract

Summary:Excessive fat accumulation in the liver is a common metabolic disorder seen in humans and animals. Fatty liver was induced in the rat by feeding the animals witfr a sucrose rieh diet containing l % orotic acid for 2-3 weeks.In the sera from fatty liver rats there were significant changes in the level of alanine aminotransferase (+ 68.7%), malic dehydrogenase (+ 77.8%), -glutamyl transpeptidase (-53.4%) and total lipids (+ 26.6%). There were small to no changes in the levels of aspartate aminotransferase, glucose-6-phosphate dehydrogenase, lactic dehydrogenase, aldolase, malic enzyme, 6-phosphogluconic acid dehydrogenase, alka-[ | line phosphatase and albumin. | » In fatty liver, significant differences were seen in the levels of glucose 6-phosphate dehydrogenase (-f 235%), l * malic enzyme (+ 170%), -glutamyl transpeptidase (+ 113%), 6-phosphogluconate dehydrogenase (+ 63%), aspartate aminotransferase (+ 35.6%), malic dehydrogenase (+ 38%), lactic dehydrogenase (+ 37%), and alanine aminotransferase (-23%).Comparison of the non-fatty part with the fatty part of the fatty liver showed larger changes in the nonfatty part of the liver, suggesting that during the fattening process, there is an induction of enzymes in the liver reaching a peak prior to lipid accumulation, declining thereafter during liver fattening. The increase in NADPH-generating lipogenic enzymes suggests that accumulated fat in the liver is at least partially from ! de^-novo increased synthesis in the liver.

SUMMARYStudying biochemical changes in the blood and liver of geese during cramming showed significant increases in the liver enzymes: malic dehydrogenase (MDH), lactic dehydrogenase (LDH), aspartate aminotransferase (AST) and malic enzyme (ME), and a decrease in alkaline phosphatase (ALP). No significant changes were seen in the activity of isocitric dehydrogenase (ICDH), and glucose 6 phosphate dehydrogenase (G6PDH). There were significant increases in serum ME, ICDH, LDH, MDH, AST, acid phosphatase (ACP), sorbitol dehydrogenase (SDH), and total lipids and decreases in serum ALP, albumin and the haemocrit. No significant changes were seen in the activity of cholinesterase, glucose, total proteins, globulins and inorganic phosphorus. There were good correlations between liver size and the change of some of the biochemical parameters studied, which may serve as markers for the presence and degree of liver fattening. There were differences between families of gray and white geese and concentrations and activities of the blood constituents paralleled the degree of liver fattening. The possibility of using these parameters as genetic markers is discussed. No correlations were found between the liver and serum biochemical parameters. The effect of transporting the geese from the farm to the slaughter house on the levels of the blood constituents is described.