Separation of Steviol Glycosides by Hydrophilic Liquid Interaction Chromatography

Zimmermann, Benno F.; Woelwer-Rieck, Ursula; Papagiannopoulos, Menelaos

doi:10.1007/s12161-011-9229-x

Cited by 26 publications

(23 citation statements)

References 13 publications

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“…The extraction procedure and the determination of steviol glycosides (SVglys) were carried out following Zimmerman et al [26]. Steviol glycosides were analyzed using a Jasco PU980 HPLC system (JASCO Benelux B.V., Utrecht, Netherlands) coupled with a UV-visible wavelength detector.…”

Section: Steviol Glycosides Determinationmentioning

confidence: 99%

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

2021

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Stevia rebaudiana (Bertoni) is a promising medicinal and aromatic plant for Mediterranean agroecosystems given its positive agronomic attributes and interesting quality features. It has both food and pharmaceutical applications, since its leaves contain sweet-tasting steviol glycosides (SVglys) and bioactive compounds, such as phenolics, flavonoids, and vitamins. We evaluated the agronomic and qualitative performances of nine stevia genotypes cultivated, in open field conditions, for two consecutive years under the Mediterranean climate of central Italy. Growth, biomass production, and accumulation of bioactive compounds (SVglys, polyphenols, and their related antioxidant activities) were evaluated, considering the effect of harvest time and crop age (first and second year of cultivation). The results showed high variability among genotypes in terms of both morpho-productive and phytochemical characteristics. In general, greater leaf dry yields, polyphenol accumulation, and antioxidant activities were found in the second year of cultivation, harvesting the plants in full vegetative growth. On the other hand, total SVglys leaf content reached the highest values in the first year when plants were at the beginning of the reproductive phase. On the other hand, although the SVglys profile (Rubusoside, Dulcoside A, Stevioside, Rebaudioside A, C, D, E, and M) remained stable over harvest times, it differed significantly depending on the crop age and genotype. Our findings provide useful information on the influence of crop age and harvest time in defining quanti-qualitative traits in stevia, with PL, SL, BR5, and SW30 being the best performing genotypes and thus suitable for breeding programs. Our study highlighted that stevia, in the tested environment, represents a promising semi-perennial crop which offers new solutions in terms of cropping system diversification and marketing opportunities.

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Section: Steviol Glycosides Determinationmentioning

confidence: 99%

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

2021

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“…Steviol glycosides were quantified by HPLC as published elsewhere (Morlock et al, 2014;Zimmermann et al, 2011). Briefly, steviol glycosides were analyzed by HPLC-UV using a Hilic column (Nucleodur Hilic, 125 mm Â 2.1 mm, 3 mm particle size; Macherey-Nagel, Düren, Germany) with a gradient elution and acetonitrile and water as eluents.…”

Section: Steviol Glycosides Quantificationmentioning

confidence: 99%

Study ofStevia rebaudianaBertoni antioxidant activities and cellular properties

Bender¹,

Graziano²,

Zimmermann³

2015

International Journal of Food Sciences and Nutrition

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The aim of our study was to determine the antioxidant activities, cytotoxicity and proliferative properties in Stevia rebaudiana leaves and stems. Leaves extracts exhibited a higher antioxidant activity than stems extract, through oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. Stevioside and rebaudioside A, the main sweetening metabolites in stevia leaves, exhibited a low ORAC value in comparison with plant extracts, while did not elicit any CAA. Stevia rebaudiana did not exhibit toxicity against HepG2 (hepatocellular carcinoma) human cells. No proliferative nor catalase modulations were observed in cells treated with such extracts. Our findings support the promising role of stevia that, apart from its sweetness, can act as a source of antioxidants, even at the intracellular level. This activity makes S. rebaudiana crude extract an interesting resource of natural sweetness with antioxidant properties which may find numerous applications in foods and nutritional supplements industries.

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“…[33,34] The growing interest in the use of steviol glycosides has motivated research aimed to characterize and separate these sweetening agents. [35][36][37][38] For separation, high-performance liquid chromatography (HPLC) using amino, [39][40][41] C18 [42,43] and HILIC columns [44,45] is considered the method of choice. Detection has been performed by optical spectroscopy [46] and MS, [47][48][49][50] including direct approaches using desorption/ionization ambient MS techniques.…”

Section: Introductionmentioning

confidence: 99%

Separation of glycosidic catiomers by TWIM‐MS using CO₂ as a drift gas

Bataglion

Souza²,

Heerdt

et al. 2015

J. Mass Spectrom.

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Traveling wave ion mobility mass spectrometry (TWIM-MS) is shown to be able to separate and characterize several isomeric forms of diterpene glycosides stevioside (Stv) and rebaudioside A (RebA) that are cationized by Na(+) and K(+) at different sites. Determination and characterization of these coexisting isomeric species, herein termed catiomers, arising from cationization at different and highly competitive coordinating sites, is particularly challenging for glycosides. To achieve this goal, the advantage of using CO2 as a more massive and polarizable drift gas, over N2, was demonstrated. Post-TWIM-MS/MS experiments were used to confirm the separation. Optimization of the possible geometries and cross-sectional calculations for mobility peak assignments were also performed.

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Separation of Steviol Glycosides by Hydrophilic Liquid Interaction Chromatography

Cited by 26 publications

References 13 publications

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

Study ofStevia rebaudianaBertoni antioxidant activities and cellular properties

Separation of glycosidic catiomers by TWIM‐MS using CO₂ as a drift gas

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Separation of Steviol Glycosides by Hydrophilic Liquid Interaction Chromatography

Cited by 26 publications

References 13 publications

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

Study ofStevia rebaudianaBertoni antioxidant activities and cellular properties

Separation of glycosidic catiomers by TWIM‐MS using CO2 as a drift gas

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Separation of glycosidic catiomers by TWIM‐MS using CO₂ as a drift gas