Separation of withanolides by high-pressure liquid chromatography with coiled columns

Hunter, Irving R.; Walden, Mayo K.; Heftmanń, Erich; Glotter, E.; Kirson, I.

doi:10.1016/s0021-9673(00)95472-4

Cited by 18 publications

(5 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The medicinal properties are attributed to a group of steroids called withanolides which are ergostane type steroids with atoms C-22 and C-26 bridged by a δ-lactone and an oxidized C-1 position. Besides a thin-layer chromatography (TLC) method for the quantification of withaferin A (Gupta et al 1996), few HPLC methods are described in literature (Bessalle and Lavie 1987, Hunter et al 1979, Banerjee et al 1994. Recently, a quantification of withanolide D and withaferine A was developed using HPLC (Ganzera et al 2003).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Indirect regeneration of Withania somnifera and comparative analysis of withanolides in in vitro and greenhouse grown plants

Dewir¹,

Chakrabarty²,

Lee³

et al. 2010

Biologia plant.

View full text Add to dashboard Cite

The present study reports an efficient protocol for indirect shoot organogenesis and plantlets regeneration of Withania somnifera (L.) Dunal. Leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA). The highest callus induction rate (89.5 %) and shoot regeneration rate (92 %) were obtained when 2 mg dm -3 BAP was combined with 0.5 mg dm -3 IAA. Three major withanolides (withaferine A, 12-deoxywithastramonolide and withanolide A) were investigated in different plant organs from in vitro and greenhouse grown plants. Leaves contained higher contents of withanolides and phenolics than roots or stems, whereas roots contained the highest contents of flavonoids and polysacharides. In vitro grown plants contained greater contents of phenolics, flavonoids and polysaccharides while lower contents of withanolides than greenhouse grown plants.

show abstract

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Indirect regeneration of Withania somnifera and comparative analysis of withanolides in in vitro and greenhouse grown plants

Dewir¹,

Chakrabarty²,

Lee³

et al. 2010

Biologia plant.

View full text Add to dashboard Cite

show abstract

“…In earlier work (42), withaferin A and several derivatives were separated on a 30-cm µ-Porasil column with ethyl acetate-hexane mixtures and were detected by a refractive index detector. Later Porasil A with hexane-isopropanol mixtures and a variable-wavelength detector were used to achieve good separation (43).…”

mentioning

confidence: 99%

Recent Developments in Naturally Occurring Ergostane-Type Steroids. A Review

Kirson¹,

Glotter²

1981

J. Nat. Prod.

Self Cite

View full text Add to dashboard Cite

“…Total withaferine A in multiple shoot culture of ashwagandha was reported to be 0.16% on an air-dry basis [7]. A high performance liquid chromatographic [HPLC] method has been reported for analysis of Withaferine A in extracts [8]. In the present paper, an accurate HPTLC method for quantitative determination of withaferine A in herbal extract and polyherbal formulations is described.…”

Section: Introductionmentioning

confidence: 95%

HPTLC analysis of Withaferine A from an herbal extract and polyherbal formulations

Mahadevan¹,

Rahul

Subburaju

et al. 2003

J of Separation Science

View full text Add to dashboard Cite

Withania somnifera has been used in Ayurvedic medicine for treatment of depression and inflammation, and as an aphrodisiac. It contains many phytochemicals such as Withaferine A, withanine, anahygrine, tropine, and withanolides. Of these, withaferine A is considered to be the most active compound. Withaferine A was estimated in herbal extract and polyherbal formulations by high performance thin layer chromatography (HPTLC). As there is no official HPTLC protocol for quantitation of the above phytochemicals, an attempt was made to quantify withaferine A in herbal extract and polyherbal formulations produced from Withania somnifera. Precoated silica gel G (aluminium backed) plates were used as stationary phase and toluene:ethyl acetate: formic acid (50 : 15 : 5) was used as mobile phase. Detection and quantification were performed by densitometry at k 213 nm. The linear range was 1 lg to 3 lg. This HPTLC method was found to be reproducible, accurate, and precise.

show abstract

Separation of withanolides by high-pressure liquid chromatography with coiled columns

Cited by 18 publications

References 3 publications

Indirect regeneration of Withania somnifera and comparative analysis of withanolides in in vitro and greenhouse grown plants

Indirect regeneration of Withania somnifera and comparative analysis of withanolides in in vitro and greenhouse grown plants

Recent Developments in Naturally Occurring Ergostane-Type Steroids. A Review

HPTLC analysis of Withaferine A from an herbal extract and polyherbal formulations

Contact Info

Product

Resources

About