Separation strategies of plant constituents–current status

Nyiredy, Szabolcs

doi:10.1016/j.jchromb.2004.08.046

Cited by 25 publications

(9 citation statements)

References 56 publications

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“…The raw impure extracts contain almost pure artemisinin, hence HPLC-UV analysis, as well as TLC densitometry, was carried out on these samples. In fact Nyiredy (2004) suggests in the requirements for separation strategies of plant constituents that the application of two different methods is necessary for correct identification. For quantitative work the two independent methods agree if the results are within 3% of each other.…”

Section: Precision and Accuracymentioning

confidence: 99%

Development and validation of a simple thin layer chromatographic method for the analysis of artemisinin in Artemisia annua L. plant extracts

Marchand

Atemnkeng

Vanermen³

et al. 2007

Biomedical Chromatography

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Owing to the development of parasite resistance to standard antimalarial treatments like chloroquine and sulfadoxine-pyrimethamine, the demand for Artemisia annua, a key ingredient for new and highly effective antimalarial drugs, is huge. Therefore selective and precise methods to determine the content of artemisinin in dry plant material and in raw impure extracts are needed. In this work a method is described for the clear separation and extraction of artemisinin from other plant components in the Artemisia annua L. plant by thin-layer chromatography (TLC). To obtain optimal extraction and recovery efficiency, several parameters were evaluted, including choice of extraction solvent, TLC plate type and sensitivity between UV and visible light. Method validation was performed on both the dry plant material and non-purified plant extracts. Toluene presented the highest extraction efficiency compared with petroleum ether, hexane and methanol. Reversed-phase plates showed more concentrated spots than normal-phase plates, while the sensitivity of the analysis in UV was comparable to that in visible light but less precise. The impure plant extracts were analyzed by both TLC and HPLC-UV at 215 nm and both methods met the requirements for linearity, selectivity, precision and accuracy. Hence, the proposed TLC method can easily be used for both qualitative and quantitative control of the raw plant extract in areas where advanced methods are scarce.

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Section: Precision and Accuracymentioning

confidence: 99%

Development and validation of a simple thin layer chromatographic method for the analysis of artemisinin in Artemisia annua L. plant extracts

Marchand

Atemnkeng

Vanermen³

et al. 2007

Biomedical Chromatography

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“…The method of choice for the chemical analysis of volatile compounds from food remains gas chromatography (GC), preferably with a mass spectrometric (MS) detector. Other available techniques for some compounds are HPLC and electronic noses (d'Acampora Zellner et al, 2008;Nyiredy, 2004;Stephan, Bücking, & Steinhart, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…The most mild, but effective approach, to allow for better penetration of solvent in the sample, seems to be ultrasound-assisted extraction (USE) (Jerković et al, 2007). Choice of solvent is the main factor influencing the chemical profile of extracted compounds (Nyiredy, 2004). Supercritical fluid extraction (SFE) may be regarded as a form of SE with supercritical CO 2 as solvent, which completely evaporates afterwards.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of isolation methods for the determination of buckwheat volatile compounds

et al. 2010

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“…A broad range of sample preparation techniques (e.g., extraction to gas, liquid or solid phase; distillation; dispersion; membrane filtration; centrifugation; and precipitation) are currently used for this purpose [1,2]. Pharmacopoeia formulae of plant material analysis mainly report the application of liquid extraction methods such as Soxhlet extraction, percolation, maceration, and extraction under reflux [2 -5].…”

Section: Introductionmentioning

confidence: 99%

Static and dynamic superheated water extraction of essential oil components from Thymus vulgaris L.

Dawidowicz

Rado

Wianowska

2009

J of Separation Science

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Superheated water extraction (SWE) performed in both static and dynamic condition (S-SWE and D-SWE, respectively) was applied for the extraction of essential oil from Thymus vulgaris L. The influence of extraction pressure, temperature, time, and flow rate on the total yield of essential oil and the influence of extraction temperature on the extraction of some chosen components are discussed in the paper. The SWE extracts are related to PLE extracts with n-hexane and essential oil obtained by steam distillation. The superheated water extraction in dynamic condition seems to be a feasible option for the extraction of essential oil components from T. vulgaris L.

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Separation strategies of plant constituents–current status

Cited by 25 publications

References 56 publications

Development and validation of a simple thin layer chromatographic method for the analysis of artemisinin in Artemisia annua L. plant extracts

Development and validation of a simple thin layer chromatographic method for the analysis of artemisinin in Artemisia annua L. plant extracts

Comparison of isolation methods for the determination of buckwheat volatile compounds

Static and dynamic superheated water extraction of essential oil components from Thymus vulgaris L.

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