Separations of enantiomers by preparative capillary isotachophoresis

Kaniansky, Dušan; Šimuničová, E.; Ölvecká, Eva; Ferancová, Adriana

doi:10.1002/(sici)1522-2683(19990901)20:13<2786::aid-elps2786>3.0.co;2-v

Cited by 35 publications

(22 citation statements)

References 30 publications

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“…Kaniansky et al [477] and Hoffmann et al [478] described ITP -systems for preparative isolation and purification of enantiomers. Coupled ITP-CZE systems for sample pretreatment and chiral separation were developed by Dankova et al [479], Fanali et al [480] and Tousaint et al [481].…”

Section: Isotachophoresis (Itp) and Isoelectric Focusing (Ie-f)mentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

226

135

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This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

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Section: Isotachophoresis (Itp) and Isoelectric Focusing (Ie-f)mentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

226

135

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“…better than 99.99% have been achieved in the Octopus CFFE system [118]. Preparative-scale separations of enantiomers by ITP has been recently described by Kaniansky et al [205] obtaining mg amounts of pure enantiomers and by recycling ITP described by Bier [30]. Flow devices utilizing isoelectric discriminating membranes (like the IsoPrime isoelectric membrane-based multicompartment electrolyzers) were developed by Righetti et al [206,207].…”

Section: Semipreparative-scale Applicationsmentioning

confidence: 99%

Fundamental aspects of chiral separations by capillary electrophoresis

2001

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A review is presented that surveys the basic theory of direct separation of enantiomers by capillary electrophoretic (CE) techniques. These separations are based on the formation of diastereomeric complexes between the enantiomeric analytes and a chiral selector added to the electrolyte solution. The review covers a comprehensive treatment of the equations needed for optimization of selectivity coefficients, resolution and analysis time in the zone electrophoretic mode. In this context, it takes into account combined equilibria of complexation and protonation/deprotonation as well as complexation and paritition into micelles. On the basis of these equations, the benefits of charged selectors and the optimization potential inherent to pH tuning can be documented. In addition, the review deals with some basic aspects of chiral isoelectric focusing and briefly discusses indirect enantioseparation. In a subsequent section a survey is given on particularfeatures of the various types of chiral selectors. Finally, the recent developments in preparative enantioseparation in continuous free-flow system and by use of isoelectric membranes are discussed.

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“…This isotachopherogram corresponds to a maximum load of the racemic mixture on the chip (a 0.6 nmol amount of tryptophan) that still could be resolved into the enantiomers in the first channel with the aid of the leading electrolyte L1 (Table 1). Such a sample load reflects a high production rate [45] attainable in the single-column ITP separations (about 2 pmols of a pure analyte produced per second). However, despite this, the use of this technique to simultaneous determination of the enantiomers was less beneficial as these could be quantified only within a narrow concentration range (less than one order of magnitude).…”

Section: Itp Separations In the Single-column And Tandem-coupled Columnsmentioning

confidence: 99%

Isotachophoresis separations of enantiomers on a planar chip with coupled separation channels

et al. 2001

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The use of a poly(methylmethacrylate) chip, provided with a pair of on-line coupled separation channels and on-column conductivity detectors, to isotachophoresis (ITP) separations of optical isomers was investigated. Single-column ITP, ITP in the tandem-coupled columns, and concentration-cascade ITP in the tandem-coupled columns were employed in this investigation using tryptophan enantiomers as model analytes. Although providing a high production rate (about 2 pmol of a pure tryptophan enantiomer separated per second), single-column ITP was found suitable only to the analysis of samples containing the enantiomers at close concentrations. A 94-mm separation path in ITP with the tandem-coupled separation channels made possible a complete resolution of a 1.5 nmol amount of the racemic mixture of the enantiomers. However, this led only to a moderate extension of the concentration range within which the enantiomers could be simultaneously quantified. The best results in this respect were achieved by using a concentration-cascade of the leading anions in the tandem-coupled separation channels. Here, a high production rate, favored in the first separation channel, was followed by the ITP migration of the enantiomers in the second channel under the electrolyte conditions enhancing their detectabilities. In dependence on the migration configuration of the enantiomers, this technique made possible their simultaneous determinations when their ratios in the loaded sample were 35:1 or less (D-tryptophan a major constituent) and 70:1 or less (L-tryptophan a major constituent).

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Separations of enantiomers by preparative capillary isotachophoresis

Cited by 35 publications

References 30 publications

Chiral separation by chromatographic and electromigration techniques. A Review

Chiral separation by chromatographic and electromigration techniques. A Review

Fundamental aspects of chiral separations by capillary electrophoresis

Isotachophoresis separations of enantiomers on a planar chip with coupled separation channels

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