Septin 7 forms a complex with CD2AP and nephrin and regulates glucose transporter trafficking

Wasik, Anita A.; Polianskyte-Prause, Zydrune; Dong, Meng‐Qiu; Shaw, Andréy S.; Yates, John R.; Farquhar, Marilyn G.; Lehtonen, Sanna

doi:10.1091/mbc.e11-12-1010

Cited by 54 publications

(74 citation statements)

References 48 publications

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“…1J,K). These results are in accordance with our previous results showing that septin 7 is expressed in podocytes and tubules in the rat kidney (Wasik et al, 2012), and that expression of septin 7 is conserved in different vertebrates. Fig.…”

Section: Sept7b Is Expressed In the Brain And Pronephros In Developinsupporting

confidence: 93%

“…However, the function of septins in the kidney in vivo has not been studied; such analysis is required to test the relevance of the functions of septins in ciliogenesis and cilia structure that have been identified in epithelial cells in tissue culture. There are two paralogous septin 7 genes in zebrafish, sept7a and sept7b, and we have reported previously that septin 7 is expressed in kidney epithelial cells in mammals (Wasik et al, 2012). Here, we show that knockdown of sept7b in zebrafish results in specific developmental defects, indicating that sept7a does not functionally compensate for sept7b.…”

Section: Introductionmentioning

confidence: 53%

“…Septin 2 and 7 localize at the base of the primary cilium of epithelial cells in tissue culture and Xenopus epidermis, and expression of the complex is required for ciliogenesis (Ghossoub et al, 2013;Hu et al, 2010;Kim et al, 2010) and as a ciliary membrane protein diffusion barrier (Chih et al, 2011;Hu et al, 2010). Septin 7 is expressed in glomerular epithelial cells (podocytes) in mouse and rat, and regulates glucose transporter trafficking in cultured podocytes (Wasik et al, 2012). Septin 7 interacts with nephrin and CD2-associated protein (CD2AP) (Wasik et al, 2012), both of which are expressed in podocytes and are essential for kidney ultrafiltration function (Kestilä et al, 1998;Shih et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Septin 7 is expressed in glomerular epithelial cells (podocytes) in mouse and rat, and regulates glucose transporter trafficking in cultured podocytes (Wasik et al, 2012). Septin 7 interacts with nephrin and CD2-associated protein (CD2AP) (Wasik et al, 2012), both of which are expressed in podocytes and are essential for kidney ultrafiltration function (Kestilä et al, 1998;Shih et al, 1999). In cultured hippocampal neurons, septin 7 regulates dendritic branching and the morphology of dendritic spines during neuronal maturation (Hu et al, 2012;Tada et al, 2007;Xie et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Sept7bis essential for pronephric function and development of left-right asymmetry in zebrafish embryogenesis

Dash

Lehtonen

Wasik

et al. 2014

Journal of Cell Science

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The conserved septin family of filamentous small GTPases plays important roles in mitosis, cell migration and cell morphogenesis by forming scaffolds and diffusion barriers. Recent studies in cultured cells in vitro indicate that a septin complex of septin 2, 7 and 9 is required for ciliogenesis and cilia function, but septin function in ciliogenesis in vertebrate organs in vivo is not understood. We show that sept7b is expressed in ciliated cells in different tissues during early zebrafish development. Knockdown of sept7b by using morpholino antisense oligonucleotides caused misorientation of basal bodies and cilia, reduction of apical actin and the shortening of motile cilia in Kupffer's vesicle and pronephric tubules. This resulted in pericardial and yolk sac edema, body axis curvature and hydrocephaly. Notably, in sept7b morphants we detected strong leftright asymmetry defects in the heart and lateral plate mesoderm (situs inversus), reduced fluid flow in the kidney, the formation of kidney cysts and loss of glomerular filtration barrier function. Thus, sept7b is essential during zebrafish development for pronephric function and ciliogenesis, and loss of expression of sept7b results in defects that resemble human ciliopathies.

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Section: Sept7b Is Expressed In the Brain And Pronephros In Developinsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sept7bis essential for pronephric function and development of left-right asymmetry in zebrafish embryogenesis

Dash

Lehtonen

Wasik

et al. 2014

Journal of Cell Science

Self Cite

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“…It also helps in glucose transporter trafficking. Septin 7 regulates glucose transporter trafficking by forming a complex with CD2AP and nephrin (Wasik et al, 2012). It is known to function in gliomagenesis as well as in suppression of glioma cell growth ).…”

Section: Functionmentioning

confidence: 99%

SEPT7 (septin 7)

Arman¹,

Bozgeyik²,

İğci³

et al. 2017

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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Characterization of septin expression in normal and fibrotic kidneys

Neubauer

Seidl

et al. 2018

Cytoskeleton

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Chronic kidney disease (CKD) is characterized by the loss of nephrons and worsening organ-fibrosis that leads to deterioration and ultimately the total breakdown of kidney function. Renal fibrosis has become a major public health problem worldwide and necessitates hemodialysis and kidney transplantation in affected patients. CKD is mainly characterized by the activation and proliferation of interstitial fibroblasts and by excessive synthesis and accumulation of extracellular matrix components, causing the disruption of the normal tissue architecture of the kidney. Septins are GTPase proteins associated with membranes, actin filaments, and microtubules and are undoubtedly crucial for cytoskeleton organization. Although some septins are involved in liver fibrosis, they have not been investigated in the context of renal fibrosis. Here, we show that numerous septins are expressed in the healthy kidney and demonstrate in fibrotic mouse kidneys that various septins are remarkably up-regulated in the tubulointerstitium compared to contralateral control kidneys. We observed the same findings in human fibrotic kidneys. In both healthy and fibrotic kidneys, septins are coexpressed with extracellular matrix components, reinforcing the structural function of septins as cytoskeletal components. Furthermore, we could show in septin 8-deficient mice that septin 8 is dispensable for the formation of renal fibrosis, and that no other septin was compensatory changed in kidneys compared to wild-type mice.

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Septin 7 forms a complex with CD2AP and nephrin and regulates glucose transporter trafficking

Cited by 54 publications

References 48 publications

Sept7bis essential for pronephric function and development of left-right asymmetry in zebrafish embryogenesis

Sept7bis essential for pronephric function and development of left-right asymmetry in zebrafish embryogenesis

SEPT7 (septin 7)

Characterization of septin expression in normal and fibrotic kidneys

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