Sequence analysis and population data on the ‘new’ short tandem repeat locus D5S2360

Henke, Lotte; Fimmers, Rolf; Reinhold, J.; Dülmer, Marlies; Cleef, S.; Arnold, Johann; Henke, Jürgen

doi:10.1016/s0379-0738(00)00349-2

Cited by 2 publications

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“…The analysis of the sequenced fragments showed that the alleles are composed of two flanking regions (including the primer sites) and two variable blocks, which were separated by a consensus sequence (see Table 1). In the first block there were (AAAG) 5 (AG) 1 (AAAG) [3][4] repeats and in the second block [(AAAG) 2 (AG) 1 ] 0-1 [(AAA-G) 6 (AG) 1 ] 0-1 (AAAG) n repeats. The size of the alleles ranged from 232 to 290 bp.…”

Section: Sequence Structure and Variation Of D17s2266e Allelesmentioning

confidence: 99%

“…KEYWORDS: forensic science, DNA typing, DNA polymorphism, short tandem repeat marker, D17S2266E, HumGH@ Microsatellite short tandem repeat (STR) markers have been used for personal identification purposes for over 10 years and fulfill this function exceptionally well. Although many STR markers have been precisely described and are routinely used, many more are being identified (1)(2)(3)(4).…”

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Sequence Analysis of a New Short Tandem Repeat Locus D17S2266E Located in the Human Growth Hormone Gene Cluster HumGH@*

Spólnicka

Jagiełło

Sołtyszewski

et al. 2008

Journal of Forensic Sciences

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D17S2266E is a new, variable genetic marker exhibiting polymorphism of the number of repeats of four- and two-nucleotide motifs. This study, carried out on a group of 250 unrelated persons from various regions of Poland, revealed the presence of 24 different alleles ranging in size from 232 to 290 base pairs. Analysis of the sequenced fragments demonstrated that the alleles consisted of two flanking regions and two variable blocks that were separated by a consensus sequence. There were (AAAG)(5)(AG)(1)(AAAG)(3-4) repeats in the first block, and [(AAAG)(2)(AG)(1)](0-1)[(AAAG)(6)(AG)(1)](0-1)(AAAG)(n) repeats in the second block. On the basis of the allele frequencies in the population, we were able to do biostatistical calculations, which gave the following results: expected heterozygosity 0.8947 +/- 0.0137, power of discrimination 0.9793, polymorphism information content 0.8837, probability of exclusion (PE) 0.7859, PE for motherless cases 0.6473, and an average paternity index of 4.7470. These biostatistical parameters show that the marker D17S2266E can find a wide range of applications in forensic testing.

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Section: Sequence Structure and Variation Of D17s2266e Allelesmentioning

confidence: 99%

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confidence: 99%