Sequence analysis of cDNAs encoding precursors of starfish asterosaps

Matsumoto, Midori; Briones, Annabelle V.; Nishigaki, Takuya; Hoshi, Motonori

doi:10.1002/(sici)1520-6408(1999)25:2<130::aid-dvg7>3.0.co;2-8

Cited by 18 publications

(15 citation statements)

References 20 publications

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“…SAPs function as moderate, though not complete, species-specific molecules since species that belong to the same taxonomic order share the same class of SAPs (Suzuki, 1995). One sea urchin species can produce different SAP isoforms; one gene encodes multiple SAPs, which are connected in tandem with a spacer amino acid (or several amino acids) forming a large precursor protein (Matsumoto et al, 1999;Ramarao et al, 1990). Interestingly, speract and its isoforms are not synthesized in the oocyte but in the accessory cells that surround the oocyte (Suzuki, 1995), in contrast to the starfish Asterias amurensis where SAPs, called asterosaps, are synthesized by the oocyte itself (Matsumoto et al, 1999).…”

Section: Sperm-activating Peptidesmentioning

confidence: 99%

Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility

Darszon¹,

Guerrero²,

Galindo³

et al. 2008

Int. J. Dev. Biol.

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100

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Section: Sperm-activating Peptidesmentioning

confidence: 99%

Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility

Darszon¹,

Guerrero²,

Galindo³

et al. 2008

Int. J. Dev. Biol.

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100

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“…Speract is synthesized by the follicle cells, not the oocytes and is incorporated into egg jelly (Darszon et al, 2008). In other echinoderms such as starfish, sperm-activating peptides are secreted by oocytes (Matsumoto et al, 1999). A mixture of peptides called asterosaps from the starfish Asterias amurensis appear to function as sperm chemoattractants (Nishigaki et al, 1996; Bohmer et al, 2005).…”

Section: Oocyte To Sperm Communicationmentioning

confidence: 99%

Advancing biology with a growing worm field

Han¹

2010

Developmental Dynamics

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“…The structural template for the guanylate cyclase from A. amurensis was searched using its primary sequence (Matsumoto et al 1999). Modeling was performed with SWISS-MODEL (Kopp and Schwede 2006) and Phyre (Kelley et al 2000).…”

Section: Molecular Modelingmentioning

confidence: 99%

Identification of Guanylate Cyclases and Related Signaling Proteins in Sperm Tail from Sea Stars by Mass Spectrometry

et al. 2008

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Marine invertebrates employ external fertilization to take the advantages of sexual reproduction as one of excellent survival strategies. To prevent mismatching, successful fertilization can be made only after going though strictly defined steps in the fertilization. In sea stars, the fertilization process starts with the chemotaxis of sperm followed by hyperactivation of sperm upon arriving onto the egg coat, and then sperm penetrate to the egg coat before achieving the fusion. To investigate whether the initiation of chemotaxis and the following signaling has species specificity, we conducted comparative studies in the protein level among sea stars, Asterias amurensis, A. forbesi, and Asterina pectinifera. Since transcription of messenger ribonucleic acid (mRNA) has been suppressed in gamete, the roles of sperm proteins during the fertilization cannot be investigated by examining the mRNA profile. Therefore, proteomics analysis by mass spectrometry was used in this study. In sea stars, upon receiving asteroidal sperm-activating peptide (asterosap), the receptor membrane-bound guanylate cyclases in the sperm tail trigger sperm chemotaxis. We confirmed the presence of membrane-bound guanylate cyclases in the three sea star species, and they all had the same structural domains including the extracellular domain, kinase-like domain, and guanylate cyclase domain. The majority of peptides recovered were from α-helices distributed on the solvent side of the protein. More peptides were recovered from the intracellular domains. The transmembrane domain has not been recovered. The functions of the receptors seemed to be conserved among the species. Furthermore, we identified proteins that may be involved in the guanylate cyclase-triggered signaling pathway.

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Sequence analysis of cDNAs encoding precursors of starfish asterosaps

Cited by 18 publications

References 20 publications

Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility

Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility

Advancing biology with a growing worm field

Identification of Guanylate Cyclases and Related Signaling Proteins in Sperm Tail from Sea Stars by Mass Spectrometry

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