2021
DOI: 10.1016/j.dnarep.2021.103213
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Sequence context effects of replication of Fapy•dG in three mutational hot spot sequences of the p53 gene in human cells

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Cited by 12 publications
(41 citation statements)
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“…To obtain structural insights into Fapy•dG opposite dA (Fapy•dG:dA), we generated a host− guest complex crystal of a Fapy•dG:dA that diffracted to 2.32 Å in space group P2 1 (Supporting Information Table S1). [10][11][12]40 In contrast to Fapy•dG:dC, and perhaps any other lesion containing duplex, Fapy•dG adopts the αand βconfigurations when paired with dA (Figure 1d,e). The presence of both anomers also distinguishes the structures of Fapy•dG:dA from the nonepimerizable analogues.…”
Section: ■ Resultsmentioning
confidence: 99%
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“…To obtain structural insights into Fapy•dG opposite dA (Fapy•dG:dA), we generated a host− guest complex crystal of a Fapy•dG:dA that diffracted to 2.32 Å in space group P2 1 (Supporting Information Table S1). [10][11][12]40 In contrast to Fapy•dG:dC, and perhaps any other lesion containing duplex, Fapy•dG adopts the αand βconfigurations when paired with dA (Figure 1d,e). The presence of both anomers also distinguishes the structures of Fapy•dG:dA from the nonepimerizable analogues.…”
Section: ■ Resultsmentioning
confidence: 99%
“…14,45,46 Both 8-oxodGuo and Fapy•dG bypass in human cells also produce measurable levels of G → A transitions in some sequences. 11,12 To determine the contribution of Pol β to the bypass of natural Fapy•dG, we examined the replication of shuttle vectors containing the lesion in human cells. We used a single-stranded plasmid, in which the lesion cannot undergo either BER or NER, to ensure that translesion synthesis occurs in the absence of DNA repair.…”
Section: ■ Resultsmentioning
confidence: 99%
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“…Accommodation of lesions by TLS Pols can be influenced by template-strand sequence and steric hindrance between bulkier lesions and Pol active sites ( Zhao and Washington, 2017 ; Thomforde et al, 2021 ). Sequence context has also been shown to affect Pol efficiency and accuracy during TLS opposite various lesions, however the molecular basis for this effect is not well understood ( Shriber et al, 2015 ; Bacurio et al, 2021 ).…”
Section: Prelesion: the First Translesion Synthesis Polymerase Substitutionmentioning
confidence: 99%
“…Pol ζ has been shown to function as the primary “extender” Pol after the “inserter” in a “three-substitution” TLS process for certain lesions ( Shachar et al, 2009 ; Lee Y.-S. et al, 2014 ). A-family Pol θ has also displayed some “extender” activity, although its exact role is still not fully defined ( Seki et al, 2004 ; Bacurio et al, 2021 ). Recently, Sulfolobus islandicus B-family polymerase Dpo2 has been shown to act as the “extender” Pol in combination with “inserter” Pol Dpo4 during bypass of abasic sites ( Feng et al, 2021 ).…”
Section: Postlesion: Extension Past the Lesion A Possible Second Substitutionmentioning
confidence: 99%