Sequence determination of a transcribed rabbit class II gene with homology to HLA-DQ ?

LeGuern, Christian; Jd, Weissman; Pn, Marche; Jouvin‐Marche, Evelyne; Laverrière, A; Mr, Bagnato; Tj, Kindt

doi:10.1007/bf00364275

Cited by 28 publications

(7 citation statements)

References 25 publications

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“…DNA samples from selectively bred rabbits of RLA haplotypes defined by RFLP (12) were amplified using PCR primers based on the published sequence (14). The primers shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…An efficient and sensitive method of typing RLA‐DQA utilizing SSCP is described. The technique is based on comparisons of the exon 2 region, chosen because it is highly polymorphic (9, 14). Conditions for denaturing/renaturing were set to favor the formation of both single‐stranded conformers and heteroduplexes, which aid in the interpretation of typing results.…”

Section: Discussionmentioning

confidence: 99%

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Improved typing procedure for the polymorphic single‐copy RLA‐DQA gene of the rabbit reveals a new allele

2001

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The DQA gene of the rabbit major histocompatibility complex (MHC, RLA) is highly polymorphic and, in contrast to those reported for other mammalian species, is present as a single copy. These properties allow use of this gene in a method to type the class II locus of RLA by a combination of single-stranded conformational polymorphism (SSCP) and heteroduplex (HD) analysis. Familial segregation of RLA-DQA was shown and RLA class II types for rabbits of unknown pedigree were determined using migration patterns of amplified genomic DNA. Typing results were confirmed in experiments where unknown samples were mixed with products from rabbits of RLA types defined by sequence analysis. These analyses detected an RLA-DQA allele in addition to the five previously described; this new allele is designated RLA-DQA-F.

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“…DNA samples from selectively bred rabbits of RLA haplotypes defined by RFLP (12) were amplified using PCR primers based on the published sequence (14). The primers shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Improved typing procedure for the polymorphic single‐copy RLA‐DQA gene of the rabbit reveals a new allele

2001

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“…Forty nanograms of each sample DNA was used in 20-l PCRs. One set of primers amplified a 358-bp product from the CRPV L1 gene, and a second set amplified a 257-bp product from the single-copy rabbit DQ-alpha gene, an MHC class I gene (33). The rabbit DQ-alpha PCR served as a monitor for the quality and integrity of the DNA and as a quantitative reference for calculating CRPV copy number.…”

Section: Methodsmentioning

confidence: 99%

Vaccination of Rabbits with an Adenovirus Vector Expressing the Papillomavirus E2 Protein Leads to Clearance of Papillomas and Infection

Brandsma

Shlyankevich²,

Zhang³

et al. 2004

J Virol

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Cervical cancer arises from lesions caused by infection with high-risk types of human papillomavirus (HPV).Therefore, vaccination against HPV could prevent carcinogenesis by preventing HPV infection or inducing lesion regression. HPV E2 protein is an attractive candidate for vaccine development because it is required for papilloma formation, is involved in all stages of the virus life cycle, and is expressed in all premalignant lesions as well as some cancers. This study reports vaccination against E2 protein using a rabbit model of papillomavirus infection. A recombinant adenovirus (Ad) vector expressing the E2 protein of cottontail rabbit papillomavirus (CRPV) was tested for therapeutic efficacy in CRPV-infected rabbits. Primary immunization with the Ad-E2 vaccine, compared to immunization with a control Ad vector, reduced the number of papillomaforming sites from 17 of 45 to 4 of 45. After booster immunization, vaccinated rabbits formed no new papillomas versus an additional 23 papillomas in rabbits that received the control vector. Papillomas in the Ad-E2 vaccinees were significantly smaller than those in the control rabbits, and all four papillomas in the Ad-E2 vaccinated rabbits regressed. No CRPV DNA was detected either in the regression sites or in sites that did not form papillomas, indicating that the vaccination led to clearance of CRPV from all infected sites.Human papillomaviruses (HPVs) cause cervical cancer, which affects about one-half million women worldwide annually (37, 51, 56). HPV-associated disease includes anal, vulvar (56), oral and other respiratory tract cancers (20), and nearly all skin cancers in patients with epidermodysplasia verruciformis (13). HPV infection is also implicated in nonmelanoma skin cancers in immunocompetent as well as immunodeficient patients (4-6, 13, 26, 29, 40). It has been estimated that 10% of the world's tumor burden is attributable to HPV infection (57).Cervical carcinogenesis begins with benign epithelial lesions induced by HPV. Because genital HPV infection is highly prevalent, many women are at risk (41). Progression to cervical cancer typically takes more than a decade, so cytological screening can detect high-grade lesions in time for treatment. However, present treatments do not cure all lesions in all patients, and recurrence is a common sequela. Furthermore, cervical cancer has a mortality rate of 33%, clearly indicating the need for better therapy.Because premalignant lesions caused by HPV can be detected early, vaccination against HPV antigens could provide an effective therapy to induce lesion regression and prevent cancer (9-12, 45). A therapeutic vaccine could also eliminate residual HPV infection after surgical removal of a lesion (9)(10)(11)(12)45). The viral E6 and E7 oncoproteins are presently popular targets for a therapeutic HPV vaccine. These proteins stimulate cellular proliferation, promote genetic instability, and transform cells, in large part by perturbing the p53 and retinoblastoma tumor suppressor pathways (reviewed in reference 38)...

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“…The RNA-cDNA hybrids were used as PCR templates for two 30-mer synthetic oligonucleotide primers, designated as DQA1 (spanning codons 2-11 in the sense strand, 5'-AGAAITCGTGGCTGACCATGTTGGCTCCTA-3') and DQA2 (spanning codons 182-191 in the complementary strand, 5'-TGAATTCGGGGCTGGAATCTCAGG-TTCCCA-3'), which were designed to contain an Eco RI restriction site (italicized sequence) at the 5' end. The primers were designed on the basis of a consensus sequence determined from previously published DQA sequences of human, rabbit, and mouse (Horn et al 1988;LeGuern et al 1987;Benoist et al 1983). After initial denaturation (94 °C; 4 min), the PCR reaction mixture (GeneAmp Kit; US Biochemicals, Cleveland, OH) was subjected to 30 cycles of denaturation (94 °C; 1 min), annealing (50 °C; 2 min), and DNA polymerization (72 °C; 4 rain).…”

Section: Momulv Reverse Transcriptase (Bethesda Researchmentioning

confidence: 99%

Allelic variation in the DQ subregion of the canine major histocompatibility complex: I. DQA

et al. 1992

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Sequence determination of a transcribed rabbit class II gene with homology to HLA-DQ ?

Cited by 28 publications

References 25 publications

Improved typing procedure for the polymorphic single‐copy RLA‐DQA gene of the rabbit reveals a new allele

Improved typing procedure for the polymorphic single‐copy RLA‐DQA gene of the rabbit reveals a new allele

Vaccination of Rabbits with an Adenovirus Vector Expressing the Papillomavirus E2 Protein Leads to Clearance of Papillomas and Infection

Allelic variation in the DQ subregion of the canine major histocompatibility complex: I. DQA

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