Sequence-specific 1H, 13C and 15N NMR assignments of Cyclophilin A like protein from Piriformospora indica involved in salt stress tolerance

Trivedi, Dipesh Kumar; Bhatt, Harshesh; Johri, Atul Kumar; Tuteja, Narendra; Bhavesh, Neel Sarovar

doi:10.1007/s12104-012-9404-z

Cited by 18 publications

(13 citation statements)

References 9 publications

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“…50 Recently we have also reported the sequence-specific 1 H, 13 C and 15 N NMR assignments and preliminary X-ray crystallographic analysis of Cyclophilin A like protein from Piriformospora indica. 51,52 In case of the expression analysis under different developmental stages, the Arabidopsis cyclophilin genes exhibited higher response to the senescence conditions. Role of a copper chaperon has also been reported in senescence previously.…”

Section: Discussionmentioning

confidence: 99%

Genome wide analysis ofCyclophilingene family from rice and Arabidopsis and its comparison with yeast

Trivedi

Yadav²,

Vaid³

et al. 2012

Plant Signaling & Behavior

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Section: Discussionmentioning

confidence: 99%

Genome wide analysis ofCyclophilingene family from rice and Arabidopsis and its comparison with yeast

Trivedi

Yadav²,

Vaid³

et al. 2012

Plant Signaling & Behavior

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“…We calculated order parameter by analyzing NMR relaxation parameters R 1 , R 2 , and 15 N-{ 1 H} nOe. Complete sequence-specific NMR resonance assignments of the protein have been reported previously22. We had observed that chemical shifts of amide protons of L34 (10.67 ppm), D88 (11.36 ppm) and H95 (10.74 ppm) and amide nitrogen of E89 (132.8 ppm) are unusually downfield shifted.…”

Section: Resultsmentioning

confidence: 52%

“…In the 2D [ 15 N, 1 H] HSQC spectra except for minor changes in chemical shifts of surface exposed residues T55, S80 and K85 no significant backbone amide chemical shift perturbations (CSPs) were observed confirming that structural integrity of the protein is maintained even at 600 mM NaCl22.…”

Section: Resultsmentioning

confidence: 92%

“…Recently, we have identified 28 rice, 35 Arabidopsis and 8 yeast Cyps genes from their respective genomes through a genome-wide analysis16. Earlier, we have reported sequence-specific 1 H, 13 C and 15 N resonance assignments for a Cyclophilin A-like protein from Piriformospora indica (PiCypA) which was found to be upregulated during osmotic stress conditions22.…”

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confidence: 99%

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Structure of RNA-interacting Cyclophilin A-like protein from Piriformospora indica that provides salinity-stress tolerance in plants

Trivedi¹,

Bhatt

Pal

et al. 2013

Sci Rep

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Soil salinity problems are widespread around the globe with increased risk of spreading over the years. The fungus Piriformospora indica, identified in Indian Thar desert, colonizes the roots of monocotyledon plants and provides resistance towards biotic as well as abiotic stress conditions. We have identified a cyclophilin A-like protein from P. indica (PiCypA), which shows higher expression levels during salinity stress. The transgenic tobacco plants overexpressing PiCypA develop osmotic tolerance and exhibit normal growth under osmotic stress conditions. The crystal structure and NMR spectroscopy of PiCypA show a canonical cyclophilin like fold exhibiting a novel RNA binding activity. The RNA binding activity of the protein and identification of the key residues involved in the RNA recognition is unique for this class of protein. Here, we demonstrate for the first time a direct evidence of countering osmotic stress tolerance in plant by genetic modification using a P. indica gene.

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“…We solved the crystal structure of PiCypA and demonstrated that it contains a canonical cyclophilin fold (Figure 17.8) [76] (PBD ID: 4EYV). Using solution-state NMR spectroscopy we showed that PiCypA retains its structural integrity even at high concentrations of Na þ [77]. PiCypA contains no additional RNA-recognizing motif, but it still binds to RNA.…”

Section: Stabilization Of Proteins and Rnasmentioning

confidence: 99%

Protein Structure–Function Paradigm in Plant Stress Tolerance

Bhatt

Kumar

Bhavesh

2013

Climate Change and Plant Abiotic Stress Tolerance

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Abiotic stresses lead to major losses of crops and fertile land worldwide. The increased percentage of arable land affected by various kinds of abiotic stresses has intensified the need to understand the different biochemical and physiological changes experienced by plants. There is an urgent need for transgenic plants having a high yield, yet also being stress tolerant, to counter this situation. Various plant and fungal proteins have been identified and characterized at the biochemical, physiological, and molecular levels, imparting stress tolerance in various model plant systems. Understanding the molecular mechanism of these proteins at atomic resolution using structural biology approaches gives a distinct edge over traditional approaches. Various proteins involved in countering abiotic stress conditions in various model organism and their future prospects are discussed with respect to their high-resolution structural information.

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Sequence-specific 1H, 13C and 15N NMR assignments of Cyclophilin A like protein from Piriformospora indica involved in salt stress tolerance

Cited by 18 publications

References 9 publications

Genome wide analysis ofCyclophilingene family from rice and Arabidopsis and its comparison with yeast

Genome wide analysis ofCyclophilingene family from rice and Arabidopsis and its comparison with yeast

Structure of RNA-interacting Cyclophilin A-like protein from Piriformospora indica that provides salinity-stress tolerance in plants

Protein Structure–Function Paradigm in Plant Stress Tolerance

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