Sequence, topography and protein coding potential of mouse int-2: a putative oncogene activated by mouse mammary tumour virus.

Abstract

A major proportion of carcinomas induced by mouse mammary tumour virus (MMTV) show evidence for proviral activation of a cellular gene, int-2, on chromosome 7. The sequence of 7869 bp of DNA spanning the transcription unit of int-2 was determined and compared with that of a series of int-2-specific cDNA clones derived from mammary tumour RNA. The predicted positions of intron-exon boundaries, established by alignment of cDNA and chromosomal DNA sequences, indicate that the gene comprises at least three exons. An open reading frame capable of encoding a protein of 245 amino acids with an estimated mol. wt of 27 kd, is flanked by substantial non-coding segments at both 5' and 3' ends. Comparison of the chromosomal DNA sequence and the predicted amino acid sequence with available data-bases has revealed no homology to other known genes. These results are discussed in relation to the status of int-2 as a candidate proto-oncogene.

Human androgen‐induced growth factor in prostate and breast cancer cells: its molecular cloning and growth properties

“…More studies such as homologous recombination experiments will elucidate distinct biological roles of AIGF. In general, genes of the FGF family contain three exons with similar exon-intron boundaries [3,4,6,22]. Exons IV and V of the AIGF gene are well correlated with the second and third exons of other FGF genes.…”

“…Acidic and basic FGFs are prototypal and they play various important roles [2]. Int-2/FGF-3, Hst-I/FGF-4, FGF-5, and FGF-6 were originally isolated as oncogene products [3,4,5,6], and are mainly expressed at various developmental stages in the normal embryo [7,8,9,10]. Androgen-induced growth factor (AIGF)/FGF-8 has been isolated from the conditioned medium of the androgen-dependent mouse mammary Shionogi carcinoma cell line (SC-3) stimulated with testosterone [11].…”

Structure and Expression of the Rat mRNA Encoding a Novel Member of the Fibroblast Growth Factor Family

“…To amplify the FGF family cDNAs, the polymerase chain reaction (PCR) was performed for 30 cycles in a reaction mixture (25 l) containing an aliquot of the above cDNA solution, 0.05 unit/ l Taq DNA polymerase (Wako Pure Chemicals, Japan), and 5 pmol/ l each of the sense and antisense degenerate primers representing all possible codons corresponding to the consensus amino acid sequences of mouse FGF-3 and FGF-7, YL-AMNK and YNTYAS, respectively (11,12). The reaction product was fractionated by electrophoresis on an 8% polyacrylamide gel.…”

“…The amino acid sequences corresponding to amino acids 96 -101 (YLAMNK) and 126 -131 (YNTYAS) of FGF-3 are identical with those of the corresponding regions of FGF-7 (11,12). Thus, we designed degenerate oligonucleotide primers representing all possible codons corresponding to the consensus sequences to isolate the cDNA for a novel member of the FGF family by the polymerase chain reaction (PCR).…”

“…Thirty-six clones had sequences homologous to those of the FGF family. Among the FGF-related cDNA clones, 11 had sequences highly homologous to those of the mouse FGF-7 or FGF-3 cDNA (11,12), indicating that they were rat homologues of these genes. Another 25 clones had an identical sequence that was similar to but distinct from those of the nine known members of the FGF family, suggesting that these clones encode a novel member of the FGF family.…”