Sequence variation in pertussis S1 subunit toxin and pertussis genes in Bordetella pertussis strains used for the whole-cell pertussis vaccine produced in Poland since 1960

Gzyl, Anna; Augustynowicz, Ewa; Gniadek, Grzegorz; Rabczenko, Daniel; Dulny, Grażyna; J, Slusarczyk

doi:10.1016/j.vaccine.2003.12.006

Cited by 39 publications

(16 citation statements)

References 22 publications

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“…Clinical isolate persisted in the lungs of immunized mice until day 8, while the reference strain could not be recovered from the lungs (p < 0.001) at day 8 after the challenge. These results, in agreement with those published in the Netherlands 15 and Poland, 25 indicate that the divergence between the local isolates and the strains used to produce the vaccines, plays a role in protection, at least, under the conditions assayed. Concerning to pertactin, various publications have demonstrated its role in adhesion and in protective immunity.…”

Section: Functional Implications Of the Genomic Divergences Observed supporting

confidence: 91%

Pertussis epidemiology in Argentina: trends over 2004–2007

Hozbor

Mooi

Flores

et al. 2009

Journal of Infection

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Section: Functional Implications Of the Genomic Divergences Observed supporting

confidence: 91%

Pertussis epidemiology in Argentina: trends over 2004–2007

Hozbor

Mooi

Flores

et al. 2009

Journal of Infection

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“…Taking together, based on our results and those on vaccinated mice previously published [9], [10], [11], [12], [13] we propose that of the three Prn variants, Prn1 binds most efficiently to the host cells, explaining its predominance in unvaccinated populations. Vaccination with Prn1-containing vaccines may have shifted the competitive balance between Prn variants allowing non-vaccine types Prn2 and Prn3 to emerge.…”

Section: Discussionsupporting

confidence: 76%

“…Antigenic divergence has occurred between vaccine strains and clinical isolates with respect to several vaccine components; Ptx, Prn, and fimbriae [3], [6], [7], [8]. Further variation in Ptx and Prn has been shown to affect vaccine efficacy in a mouse model [9], [10], [11], [12], [13]. In addition to antigenic variation, increased Ptx production has been associated with the resurgence of pertussis [14].…”

Section: Introductionmentioning

confidence: 99%

Studies on Prn Variation in the Mouse Model and Comparison with Epidemiological Data

Gent¹,

Loo

Heuvelman³

et al. 2011

PLoS ONE

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The virulence factor pertactin (Prn) is a component of pertussis vaccines and one of the most polymorphic Bordetella pertussis antigens. After the introduction of vaccination shifts in predominant Prn types were observed and strains with the Prn vaccine type (Prn1) were replaced by strains carrying non-vaccine types (Prn2 and Prn3), suggesting vaccine-driven selection. The aim of this study was to elucidate the shifts observed in Prn variants. We show that, although Prn2 and Prn3 circulated in similar frequencies in the 1970s and 1980s, in the 1990s Prn2 strains expanded and Prn3 strains disappeared, suggesting that in vaccinated populations Prn2 strains are fitter than Prn3 strains. We established a role for Prn in the mouse model by showing that a Prn knock-out (Prn-ko) mutation reduced colonization in trachea and lungs. Restoration of the mutation resulted in a significant increase in colonization compared to the knock-out mutant. The ability of clinical isolates with different Prn variants to colonize the mouse lung was compared. Although these isolates were also polymorphic at other loci, only variation in the promoter for pertussis toxin (ptxP) and Prn were found to contribute significantly to differences in colonization. Analysis of a subset of strains with the same ptxP allele revealed that the ability to colonize mice decreased in the order Prn1>Prn2 and Prn3. Our results are consistent with the predominance of Prn1 strains in unvaccinated populations. Our results show that ability to colonize mice is practically the same for Prn2 and Prn3. Therefore other factors may have contributed to the predominance of Prn2 in vaccinated populations. The mouse model may be useful to assess and predict changes in the B. pertussis population due to vaccination.

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“…We suspect that the process of changes in vaccine strain composition in the period 1960-1978 resulted in more diverse immune pressure in the population within decades, influencing differently to elsewhere allele prevalence. Up to 1976, vaccine lots manufactured per year were different in regard to strain composition and the number of vaccine strains used was even higher than ten in some lots [31]. The last vaccine strain composition change took place in 1978 and was aimed at eliminating strains expressing the highest toxicity.…”

Section: Discussionmentioning

confidence: 99%

Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959–2013

Mosiej

Zawadka

Krysztopa-Grzybowska

et al. 2014

Eur J Clin Microbiol Infect Dis

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This study aimed to characterise Bordetella pertussis isolates circulating in Poland since 1959. Sequence analysis of ptxA, ptxC, prn, tcfA, fim2, fim3 and ptxP for 175 clinical isolates and currently and previously used vaccine strains was performed. Clinical isolates from the period 1995-2013 were found to be different to three currently used vaccine strains harbouring the allelic combination ptxA2-ptxC1-ptxP1-prn1-tcfA2-fim2-1-fim3-1, seen frequently in Poland in the early pertussis vaccination period but not found after 1995. Generally, among B. pertussis isolates from the period 2000-2013, two genotypes predominated, ptxA1-ptxC1-ptxP1-prn1-tcfA2-fim2-2-fim3-1 and ptxA1-ptxC1-ptxP1-prn2-tcfA2-fim2-1-fim3-1, with frequencies of 45% and 32.5%, respectively. The isolates harbouring ptxA1-ptxC2-ptxP3-prn2-tcfA2-fim2-1-fim3-2 and ptxA1-ptxC2-ptxP3-prn2-tcfA2-fim2-1-fim3-1 profiles, currently highly prevalent within other European Union (EU) countries, were rarely found in Poland, as they circulated in the period 2000-2013 with frequencies of 10% and 5%, respectively. We hypothesise that several previous changes of strain composition in whole-cell pertussis vaccine produced locally and used since 1960 in Poland resulted in a more diverse immune pressure in the population, resulting in different prevalence of alleles compared to elsewhere.

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Sequence variation in pertussis S1 subunit toxin and pertussis genes in Bordetella pertussis strains used for the whole-cell pertussis vaccine produced in Poland since 1960

Cited by 39 publications

References 22 publications

Pertussis epidemiology in Argentina: trends over 2004–2007

Pertussis epidemiology in Argentina: trends over 2004–2007

Studies on Prn Variation in the Mouse Model and Comparison with Epidemiological Data

Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959–2013

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