2005
DOI: 10.1016/j.fgb.2005.04.007
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Sequence variation in the CYP51 gene of Blumeria graminis associated with resistance to sterol demethylase inhibiting fungicides

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Cited by 123 publications
(117 citation statements)
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“…Sequence analysis of the CYP51 gene from the progeny of a cross between DMI-sensitive and resistant isolates demonstrated co-segregation between the mutant alleles and resistance. Consistent with other studies, the authors found that genetic analysis of resistance to the triadimenol indicates that mutation of the CYP51 gene is not the only mechanism of resistance operating in B. graminis: Two moderately resistant isolates had no mutations in the CYP51 gene, and had identical sequences to that of the sensitive isolate, suggesting that resistance in at least these two isolates must be due to a mutation in an entirely different gene (Wyand and Brown 2005). The identification of isolates such as these should serve as adequate warning that molecular detection of mutations in the structural gene may result in an underreporting of actual incidence of resistance in the field.…”
Section: Sterol Biosynthesis Inhibitorssupporting
confidence: 83%
“…Sequence analysis of the CYP51 gene from the progeny of a cross between DMI-sensitive and resistant isolates demonstrated co-segregation between the mutant alleles and resistance. Consistent with other studies, the authors found that genetic analysis of resistance to the triadimenol indicates that mutation of the CYP51 gene is not the only mechanism of resistance operating in B. graminis: Two moderately resistant isolates had no mutations in the CYP51 gene, and had identical sequences to that of the sensitive isolate, suggesting that resistance in at least these two isolates must be due to a mutation in an entirely different gene (Wyand and Brown 2005). The identification of isolates such as these should serve as adequate warning that molecular detection of mutations in the structural gene may result in an underreporting of actual incidence of resistance in the field.…”
Section: Sterol Biosynthesis Inhibitorssupporting
confidence: 83%
“…In addition, a number of reports have identified polymorphisms in the erg11 gene (which is homologous to cyp51A) from clinical Candida albicans isolates that are responsible for and/or that are associated with fluconazole resistance (16,26,43). On the other hand, the resistance of filamentous fungi to different demethylase inhibitors (DMIs) used for agricultural purposes has mainly been correlated with one amino acid change (Y136F) in the azole target, Cyp51 (11,12,53), or with its overexpression (18,23,44,46). In A. fumigatus, azole drug resistance has been described for both laboratory mutants and a Mutant nomenclature: strains CM237-TRL98H1 to CM237-TRL98H5, gene replacement of the TRL98H fragment from strain CM2627 into azole-susceptible strain CM237; strain CM237-TR7, gene replacement of the TR fragment from strain CM2627 into strain CM237; strains akuB clinical strains and has mainly been attributed to alterations in the target enzyme (Cyp51A) (2,15,25,28,32).…”
Section: Discussionmentioning
confidence: 99%
“…In plant pathogens, resistance to azoles is less common, and although some CYP51 mutations have been correlated with a reduced-azole-sensitivity phenotype (8,9,31), these have yet to be shown biochemically to affect azole binding. In M. graminicola, CYP51 mutations are common in modern populations (2,6,7,28).…”
mentioning
confidence: 99%