Sequences and transcriptional analysis ofCoffea arabicavar. Caturra andCoffea libericaplant responses to coffee berry borerHypothenemus hampei(Coleoptera: Curculionidae: Scolytinae) attack

Abstract

The coffee berry borer (CBB) is the most prevalent pest of coffee plantations. Within the Coffea genus, C. arabica is susceptible to CBB and C. liberica shows a lower susceptibility. Two EST libraries were constructed from the total RNA of C. arabica and C. liberica fruits artificially infested with CBBs for 24 h. Using 6000 clones sequenced per library, a unigene database was generated, obtaining 3634 singletons and 1454 contigs. For each contig, the proportion of sequences present in both species was determined and a differential gene expression between the species was detected. C. arabica displayed a higher relative expression of proteins involved in general stress responses, whereas C. liberica showed the induction of a higher number of insect defense proteins. In order to validate the results, quantifications through real-time PCR were done. A hevein-like protein, an isoprene synthase, a salicylic acid carboxyl methyltransferase and a patatin-like protein gene were highly upregulated in C. liberica at 24 and/or 48 h after insect infestation compared to C. arabica. The identification of metabolic pathways induced by this pest insect provides tools to take advantage of the genetic resources available for the control of CBB.

Differential Metabolic Responses Caused by the Most Important Insect Pest of Coffee Worldwide, the Coffee Berry Borer (Hypothenemus hampei)

“…However, differential expression comparisons revealed that there were also changes dependent on the genotype, demonstrating that the response to the coffee berry borer infestation is genotype-dependent. 35 Further transcriptomics studies on arabica Colombia Supremo targeting genes from the phenylpropanoid pathway could validate the results presented in this study. Higher Levels of Sugar Alcohols and Organic Acids in Infested Robusta Suggesting Host Priming.…”

“…Both species showed overexpression of genes related to ethylene production and pathogen recognition when infested by the coffee berry borer. However, differential expression comparisons revealed that there were also changes dependent on the genotype, demonstrating that the response to the coffee berry borer infestation is genotype-dependent . Further transcriptomics studies on arabica Colombia Supremo targeting genes from the phenylpropanoid pathway could validate the results presented in this study.…”

“…Caturra and on C. liberica Bull. ex Hiern infested by the coffee berry borer . Both species showed overexpression of genes related to ethylene production and pathogen recognition when infested by the coffee berry borer.…”

“…ex Hiern infested by the coffee berry borer. 35 Both species showed overexpression of genes related to ethylene production and pathogen recognition when infested by the coffee berry borer. However, differential expression comparisons revealed that there were also changes dependent on the genotype, demonstrating that the response to the coffee berry borer infestation is genotype-dependent.…”

Ecología química de interacciones entre plantas, insectos y controladores naturales de plagas herbívoras

“…En condiciones de laboratorio se encontró una disminución entre 30% -40% de frutos infestados, menor cantidad de huevos ovipositados y menor sobrevivencia de la broca en frutos de Coffea liberica y tres introducciones de Coffea arabica del banco de germoplasma del Centro Nacional de Investigaciones de Café en Colombia (Romero & Cortina, 2004). Estudios de expresión génica en frutos infestados por broca encontraron una mayor expresión de proteínas involucradas en respuestas de defensas contra insectos en C. liberica (parcialmente resistente a broca) comparadas con C. arabica (susceptible a broca) (Idárraga et al, 2012). Estas dos especies de plantas también exhiben diferencias en la composición de volátiles emitidos por sus frutos (Cruz-Roblero & Malo, 2013).…”