Sequences of Landomycin E and Carotenoid Biosynthetic Gene Clusters, and Molecular Structure of Transcriptional Regulator of Streptomyces globisporus 1912

Abstract

S. globisporus 1912 in one cluster. Twenty of these lnd genes have been sequenced for the first time. The new regulatory responce gene lndRR and the sensor kinase gene lndY1 were proposed as the members of the putative two-component system. High identity (94-95 %) was determined for the lnd genes of the 1912 strain and those of the metagenomic clone AZ97. Seven carotenoid biosynthetic genes crt of the strain 1912-4Crt were sequenced and localized in one cluster consisting of two convergent operons from 4 and 3 crt genes. High homology (93 %) of the crt gene clusters of S. globisporus 1912 and S. griseus IFO 13350 was shown. Two non-punctual repeats (NPRs) of 21 bp were identified in the sequence of crtY gene coding lycopene cyclase. It was shown that the deletion of 117 bp including the sequence between NPRs of 96 bp and one NPR from 5`-side activated the crt gene cluster and increased the production of beta carotene (6.91 mg/l) and lycopene (3.24 mg/l) by the strain 1912-4Crt. Deletion of 86 bp was revealed in the regulatory gene lndRR resulting in the deficiency of landomycin E production in the strain 1912-4Crt. The DNA sequences of crt and lnd genes of S. globisporus 1912 were submitted to the NCBI database with accession numbers KM349312 and KJ645792, respectively.S. globisporus 1912 produced a low-molecular-weight compound that, like A-factor, restored the landomycin E and streptomycin biosynthesis and sporulation of the defective mutants S. globisporus 1912-B2 and S. griseus 1439, respectively. The compound was purified by thin layer chromatography and HPLC. It had an absorption maximum at λ max = = 245 nm and a molecular mass m/z 244. On the basis of NMR spectroscopy the chemical structure of the transcriptional regulator was elucidated as the new (L)-N-methylphenylalanyl-dehydrobutyrine diketopiperazine. K e y w o r d s:Soil bacteria Streptomyces produces the majority of the known antibiotics, many of the carotenoids and transcriptional regulators [18][19][20]. The research of the new antitumor antibiotics presents an important scientific problem because of the rise of drug resistant metastatic cells from the initial tumor [4]. The

Rapid spectrophotometric detection for optimized production of landomycins and characterization of their therapeutic potential

“…[10][11][12][13] Landomycin BGCs have only been directly identified in three genomic samples, though a fourth is presumed due to landomycin detection in culture extracts. [14][15][16][17] Landomycin A (lanA) and landomycin E (lanE) are the most widely-studied landomycins, and both possess the widest reported spectrum of anticancer activities among the landomycins. 18 The structures of lanA and lanE only differ in the composition of their glycan chain.…”

Difference in sequences of the Streptomyces globisporus 1912 and 2 its mutants

“…Мутант S. globisporus 1912-2 був виділений за дії n-метил-н'-нітро-н-нітрозогуанідину на спори S. globisporus 1912 [7]. Він продукує ландоміцин Е та новий регулятор транскрипції біосинтезу антибіотиків і морфогенезу у стреп-томіцетів дикетопіперазинового характеру [7,11,12]. Мутант S. globisporus 1912-4Crt -спонтанний мутант S. globisporus 1912, що продукує бета-каротин та лікопін [10,12].…”

“…Сиквеновано [10,12]. Однак сиквенування ДНК мутанта 1912-4Crt дозволило підтвердити вірогідну послідовність у 6 п. н. між lndІ та lndЕ генами [12].…”