Sequencing and Analysis of a Calmodulin cDNA from Pea (Pisum sativum L. var Alaska)

Differential expression and functional analysis of three calmodulin isoforms in germinating pea (Pisum sativum L.) seeds

“…This MS analysis demonstrates the existence of three CaM protein isoforms in germinating pea seeds diverging from each other by single substitutions in the N‐term region of the protein: PsCaM as deduced from the published cDNA sequence (Smith et al ., 1995b) which will be referred as PsCaM1 in the following, PsCaM2 with a Ser 10 to Ala 10 substitution and PsCaM3 with a Asp 7 to Glu 7 substitution.…”

“…The size of the hybridised RNA ranged from 550 to 800 bases which is consistent with the nucleotide sequence data for P. sativum var. Alaska CaM cDNA given to be 692 bp, poly(A) tail excluded (Smith et al ., 1995b). PsCaM was found at a low level in dry whole seeds, embryonic axes and cotyledons, indicating that a small pool of transcripts remains in quiescent pea seeds.…”

“…To date, only one CaM (accession U13882) and one CaM‐like (accession U13736) cDNAs were isolated and sequenced from Pisum sativum var. Alaska (Smith et al ., 1995a,b).…”

Isolation, Sequencing, and Analysis of a Calmodulin-Like cDNA from Pea (Pisum sativum L. var Alaska)

“…The coding region of pCRAW32 exhibits the highest nucleotide sequence identity (85%) with soybean calmodulin (ScaM-5; Lee et al, 1993) and the least (70%) with pea calmodulin (pCRAW22; Smith, et al, 1995). Despite the range of diversity at the nucleotide level (66-85%), generally a11 higher plant calmodulins are highly identical (>97%) at the amino acid level, although pCRAW32 and SCaM-5 exhibit a slightly lower (88-90%) range of identities.…”