Sequencing and phylogeny of sulphonamide resistant genes by using MEGA6 software program.

Jawad, Ali; Ayyez, Hayder N.; Kleaf, Saba F.

doi:10.26452/ijrps.v9ispl1.1359

Cited by 2 publications

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“…BLAST searches were performed and homologous sequences were downloaded from the National Center for Biotechnology Information (NCBI) database (Chen et al, ). Sequences were aligned and connected using MEGA6 software (Jawad, Ayyez, & Kleaf, ). The phylogenetic location of the strains in the Diaporthales was revealed by performing a phylogenetic analysis using the ITS sequence.…”

Section: Methodsmentioning

confidence: 99%

New leaf and fruit disease of Juglans regia caused by Juglanconis juglandina in Xinjiang, China

Zhao

et al. 2019

Forest Pathology

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Juglans regia L. is an important and scarce natural resource that grows in the part of the western Tianshan Mountains and the Pamir Alai Mountains in Central Asia. They only distribute in Gongliu County, Yili, Xinjiang Uygur Autonomous Region in China. However, the healthy growth of J. regia was seriously affected by a fungal disease affecting leaves, branches and fruit in 2016. Sixty isolates were obtained from diseased branches, leaves and fruit collected. The pathogenicity of the isolates was confirmed by inoculating shoots and fruit collected from healthy J. regia in Gongliu County and by inoculating the leaves of J. regia seedlings. The pathogen was re‐isolated from all inoculated shoots, leaves and fruits, thereby fulfilling Koch's postulates. Furthermore, isolates from leaves were able to infect fruit and shoots and vice versa. The isolate 1429‐1 was more pathogenic to the branches than HG and HY, no significant difference between the other isolates from leaves and fruits. Based on morphological, cultural characteristics and multilocus analyses using the internal transcribed spacer region, the RNA polymerase II subunit 2 gene, the calmodulin gene, the guanine nucleotide‐binding protein subunit beta gene, the translation elongation factor 1‐alpha gene and the β‐tubulin gene sequences, the fungus was identified as Juglanconis juglandina. Although J. juglandina has been reported previously as the causal agent of stem disease, this is the first description of J. juglandina as the causal agent of a leaf and fruit disease of J. regia.

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Section: Methodsmentioning

confidence: 99%

New leaf and fruit disease of Juglans regia caused by Juglanconis juglandina in Xinjiang, China

Zhao

et al. 2019

Forest Pathology

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“…To construct a MIKCc-type MADS-box gene phylogeny tree using MEGA (Version 6.06) [30], aligned protein sequences from three plant species, Arabidopsis, O. Sativa, and D. latiflorus were employed. The phylogenetic tree was constructed by applying the neighbor-joining method with amino acid p-distance, and the reliability was obtained by bootstrapping 1000 times.…”

Section: Phylogenetic Tree Constructionmentioning

confidence: 99%

Genome-Wide Identification of MIKCc-Type MADS-Box Family Gene and Floral Organ Transcriptome Characterization in Ma Bamboo (Dendrocalamus latiflorus Munro)

Yang

Wan

et al. 2022

Genes

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Most bamboos die after flowering, and the molecular mechanisms responsible for flowering is poorly understood. The MIKCc-type MADS-box family gene is involved in the flowering process. To explore the mechanism of the MIKCc-type MADS-box gene and phytohormone regulation in the flowering of Dendrocalamus latiflorus Munro (D. latiflorus), characterized by extremely rapid growth and widely cultivated woody bamboo, we initially did a genome-wide analysis of the MIKCc-type MADS-box gene in D. latiflorus. In the meantime, transcriptome analysis was performed using the floral organs. A total of 170 MIKCc-Type MADS-Box genes were identified and divided into 15 categories. The cis-acting element analysis in promoters regions revealed that MIKC-type MADS-box family genes were associated with hormones, including auxin, abscisic acid (ABA), gibberellin (GA) and jasmonic acid (JA), which was found at 79, 476, 96, 486 sites and cover 61, 103, 73, 128 genes. Genome synteny analysis showed subgenome AA and BB were better than CC and obtained 49, 40, 39 synteny genes compared with Oryza sativa (O. sativa). In transcriptome analysis of floral organs, the enriched pathway from DEGs included circadian, vernalization and gibberellin pathways associated with the flowering process. We found that the jasmonic acid synthesis gene is highly expressed in the pistil, which may be the cause of Ma bamboo pollen abortion. The expression profile showed that most MIKC-type MADS-box genes exhibited high expression in flower organs. The consequences of this study will provide insight into the irregular flowering and low pollen counts of Ma bamboo.

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Sequencing and phylogeny of sulphonamide resistant genes by using MEGA6 software program.

Cited by 2 publications

References 0 publications

New leaf and fruit disease of Juglans regia caused by Juglanconis juglandina in Xinjiang, China

New leaf and fruit disease of Juglans regia caused by Juglanconis juglandina in Xinjiang, China

Genome-Wide Identification of MIKCc-Type MADS-Box Family Gene and Floral Organ Transcriptome Characterization in Ma Bamboo (Dendrocalamus latiflorus Munro)

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