Sequencing-based network analysis provides a core set of genes for understanding hemolymph immune response mechanisms against Poly I:C stimulation in Amphioctopus fangsiao

Chen, Xipan; Liu, Xiumei; Cai, Dequan; Cui, Cuiju; Yang, Jianmin; Xu, Xiaohui; Li, Zan

doi:10.1016/j.fsi.2023.108544

Cited by 4 publications

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“…The above experimental procedure and raw data obtained by sequencing were derived from Chen et al [ 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

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Weighted Gene Co-Expression Network Analysis Based on Stimulation by Lipopolysaccharides and Polyinosinic:polycytidylic Acid Provides a Core Set of Genes for Understanding Hemolymph Immune Response Mechanisms of Amphioctopus fangsiao

Wang,

Chen,

et al. 2023

Animals

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The primary influencer of aquaculture quality in Amphioctopus fangsiao is pathogen infection. Both lipopolysaccharides (LPS) and polyinosinic:polycytidylic acid (Poly I:C) are recognized by the pattern recognition receptor (PRR) within immune cells, a system that frequently serves to emulate pathogen invasion. Hemolymph, which functions as a transport mechanism for immune cells, offers vital transcriptome information when A. fangsiao is exposed to pathogens, thereby contributing to our comprehension of the species’ immune biological mechanisms. In this study, we conducted analyses of transcript profiles under the influence of LPS and Poly I:C within a 24 h period. Concurrently, we developed a Weighted Gene Co-expression Network Analysis (WGCNA) to identify key modules and genes. Further, we carried out Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to investigate the primary modular functions. Co-expression network analyses unveiled a series of immune response processes following pathogen stress, identifying several key modules and hub genes, including PKMYT1 and NAMPT. The invaluable genetic resources provided by our results aid our understanding of the immune response in A. fangsiao hemolymph and will further our exploration of the molecular mechanisms of pathogen infection in mollusks.

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“…The above experimental procedure and raw data obtained by sequencing were derived from Chen et al [ 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…This study encompasses the analysis of the transcription profiles of hemolymph tissues at 6 and 24 h under two distinct types of stimulation [ 25 , 26 ]. Employing the union of differentially expressed genes (DEGs) within a 24 h period under both stimulation types, the WGC network was constructed.…”

Section: Introductionmentioning

confidence: 99%