1993
DOI: 10.1128/aac.37.9.2037
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Sequencing of a tet(Q) gene isolated from Bacteroides fragilis 1126

Abstract: Recently, Tet Q, a tetracycline resistance determinant that confers resistance by a ribosome protection mechanism, was described and added to the two previously described classes, Tet M and Tet O. The first representative of this class, tetA(Q)1, was isolated from Bacteroides thetaiotaomicron DOT. We report the sequencing of a gene isolated from B. fragilis 1126 which also confers tetracycline resistance. Because of its high degree of identity (97%) with the tetA(Q)1 gene, we defined it as tetA(Q)2. MIC studie… Show more

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Cited by 30 publications
(16 citation statements)
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“…The DNA probe used in the present study is virtually the same probe used by Olsvik et al (28) in their study: both are a 1535-bp EcoRI-PvuU fragment of tetAiQ). The main difference was that the tetA(Q)l gene (27) was used in the study made by Olsvik et al (28), and the tetA{Q)2 gene (20) was used in the present study. The two probes have a 96%) homology.…”
Section: Discussionmentioning
confidence: 92%
See 1 more Smart Citation
“…The DNA probe used in the present study is virtually the same probe used by Olsvik et al (28) in their study: both are a 1535-bp EcoRI-PvuU fragment of tetAiQ). The main difference was that the tetA(Q)l gene (27) was used in the study made by Olsvik et al (28), and the tetA{Q)2 gene (20) was used in the present study. The two probes have a 96%) homology.…”
Section: Discussionmentioning
confidence: 92%
“…A 1535-bp Pvull-EcoBJ fragment containing an internal portion of the te-tA{Q)2 gene from B. fragilis 1126 (20) was isolated from the plasmid pBSKl.2-5 (Bluescript II SK+ Q. 2.8 kb Smal-Clal fragment, containing te-tA(Q)2, from the chromosomal DNA of B. fragilis 1126), which was kindly given to us by DG.…”
Section: Fef (Q) Probe For Dna-dna Hybridizationmentioning
confidence: 99%
“…The cotransfer of tetracycline and penicillin resistance genes in black‐pigmented strains is probably related to a conjugation system encoded by chromosomal transposons ( 16, 24, 26). The strains producing a β‐lactamase but susceptible to tetracycline could have deletion in the tetQ gene or a low‐level minimal inhibitory concentration increase not easily detectable ( 27). The past use of tetracycline in the treatment of oral infections may have contributed to develop the resistance to β‐lactam antibiotics as well, and conversely the present use of β‐lactam antibiotics may contribute to partially stabilize tetracycline resistance in oral gram‐negative anaerobic rods ( 26, 33).…”
Section: Discussionmentioning
confidence: 99%
“…Aminopenicillins are still commonly used, and these β‐lactamases cannot be ignored as a threat of inactivating β‐lactam antibiotics in the subgingival environment, thus protecting endogenous flora and other pathogens, particularly oral streptococci, during acute infections. The question is addressed as well as the potential role of this reservoir of β‐lactamase genes, and additional clinical, epidemiological and genetic surveys are necessary ( 8, 18, 21, 27, 45).…”
Section: Discussionmentioning
confidence: 99%
“…The tetracycline-resistance genes frotn Bacteroides fragilis and Bacteroides thetaiotaomierott have been sequenced (11,17), and the genes share 97.3% and 97.0% of amino acid and DNA sequences respectively (11). The /i;'/(Q) gene from pNFDI3-2 also has a 51.5% to 52.6% DNA sequence homology with the tet(M) and tet(O) genes (17).…”
mentioning
confidence: 99%