Sequential allocation and global pattern of movement of the definitive endoderm in the mouse embryo during gastrulation

Tam, Patrick; Khoo, Poh Lynn; Lewis, Samara E.; Bildsoe, Heidi; Wong, Nicole; Tsang, Tania E.; Gad, Jacqueline M.; Robb, Lorraine

doi:10.1242/dev.02724

Cited by 90 publications

(100 citation statements)

References 36 publications

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“…The dynamic expression pattern of Trh parallels a model of definitive endoderm formation recently proposed using lineage tracing and fate mapping experiments (Tam et al, 2007). At early gastrulation stages Trh expression appears to mark the lateral definitive endoderm population, which originates from the anterior end of the primitive streak at the mid-streak stage.…”

Section: Discussionsupporting

confidence: 64%

“…Numerous fate mapping studies have detailed the movement of the definitive endoderm as it emerges from the primitive streak and displaces the visceral endoderm (Beddington, 1983;Lawson et al, 1986;Lawson and Pedersen, 1987;Tam and Beddington, 1992;Tam et al, 2004Tam et al, , 2007. It has recently been shown that there are two paths of migration for the foregut precursors (Tam et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…It has recently been shown that there are two paths of migration for the foregut precursors (Tam et al, 2007). At the mid-streak stage, definitive endoderm cells emerge from the primitive streak and move toward the anterior of the embryo by way of the lateral sides of the embryo.…”

Section: Introductionmentioning

confidence: 99%

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Dynamic expression of Thyrotropin‐releasing hormone in the mouse definitive endoderm

McKnight¹,

Hou²,

Hoodless³

2007

Developmental Dynamics

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Thyrotropin-releasing hormone (TRH) is a well-characterized regulator of the hypothalamic-pituitary-thyroid endocrine axis. Here, we describe the expression of Trh during early embryonic development in the mouse. We find Trh to be highly expressed during postimplantation stages in the mouse embryo, with expression first observed in the epiblast at embryonic day (E) 6.5. During gastrulation, Trh is expressed in the newly formed definitive endoderm cells, and at embryonic day (E) 7.75, marks the entire definitive endoderm. Subsequently, Trh mRNA levels rapidly decrease such that, by E9.0, expression in the definitive endoderm is no longer detected, after which neural expression predominates. Thus, Trh is expressed dynamically and specifically in the developing mouse definitive endoderm from E7.0 to E8.5. Trh is unique among definitive endoderm markers as it transiently marks the entire definitive endoderm population and is not expressed in the extraembryonic endoderm. Trh will be a valuable tool to study definitive endoderm formation in the mouse embryo.

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Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

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Dynamic expression of Thyrotropin‐releasing hormone in the mouse definitive endoderm

McKnight¹,

Hou²,

Hoodless³

2007

Developmental Dynamics

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“…Recent fate-mapping studies have helped identify developmental fates of definitive endoderm cells during gastrulation (Tam et al, 2003;Tam et al, 2007;Franklin et al, 2008). Consistent with the fate-mapping analyses, the definitive endoderm cells expressing Ende under the head folds of the early head fold embryo are destined for the ventral aspects of the foregut, which was observed at later stages of embryos expressing Ende ( Fig.…”

Section: O)supporting

confidence: 62%

Expression of two novel transcripts in the mouse definitive endoderm

Hassan

Hou

Wei

et al. 2010

Gene Expression Patterns

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Here we describe the expression of two novel transcripts, Ende (AK014119) and Npe (AK084355), during early mouse embryogenesis. Ende mRNA was first detected at embryonic day (E) 7.0 in a small population of epiblast cells in the distal half of the embryo. At E7.5, Ende was expressed by newly formed definitive endoderm cells in the proximal half of the embryo, and was not expressed in extra-embryonic endoderm. This expression pattern then changed to the ventral aspect of the developing foregut pocket and the entire hindgut pocket at E8.0-8.5, before becoming restricted to the foregut overlying the heart and the posterior most hindgut. By E9.0 Ende expression was also observed in the posterior half of the ventral neural tube. Thus, Ende was expressed dynamically and in specific populations of the definitive endoderm from E7.0 to E8.5. We found Npe expression to be restricted to the node/posterior notochord region at the distal tip of the embryo between E7.0 and E8.0. By E9.5, Npe expression was observed in the posterior most population of dorsal hindgut cells and notochord cells. Given their expression in mouse definitive endoderm populations, Ende and Npe will be valuable tools to study formation and development of this tissue.

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“…By assaying the temporal pattern of expression of genes associated with germ layer formation in embryoid bodies (EBs) over a 4-day period, EpiSC lines were found to respond differently to the induction of differentiation. In particular, the temporal expression profile of Mixl1, a gene that is expressed in the PS and required for DE formation [22], varied across the set of EpiSCs analysed. Prior to differentiation, Mixl1 expression was comparable across all EpiSC lines analysed [17].…”

Section: Endoderm Lineage Propensity Of the Epiblast Stem Cellsmentioning

confidence: 99%

Differential response of epiblast stem cells to Nodal and Activin signalling: a paradigm of early endoderm development in the embryo

Kaufman‐Francis

Goh

Studdert

et al. 2014

Phil. Trans. R. Soc. B

Self Cite

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Mouse epiblast stem cells (EpiSCs) display temporal differences in the upregulation of Mixl1 expression during the initial steps of in vitro differentiation, which can be correlated with their propensity for endoderm differentiation. EpiSCs that upregulated Mixl1 rapidly during differentiation responded robustly to both Activin A and Nodal in generating foregut endoderm and precursors of pancreatic and hepatic tissues. By contrast, EpiSCs that delayed Mixl1 upregulation responded less effectively to Nodal and showed an overall suboptimal outcome of directed differentiation. The enhancement in endoderm potency in Mixl1-early cells may be accounted for by a rapid exit from the progenitor state and the efficient response to the induction of differentiation by Nodal. EpiSCs that readily differentiate into the endoderm cells are marked by a distinctive expression fingerprint of transforming growth factor (TGF)-β signalling pathway genes and genes related to the endoderm lineage. Nodal appears to elicit responses that are associated with transition to a mesenchymal phenotype, whereas Activin A promotes gene expression associated with maintenance of an epithelial phenotype. We postulate that the formation of definitive endoderm (DE) in embryoid bodies follows a similar process to germ layer formation from the epiblast, requiring an initial de-epithelialization event and subsequent re-epithelialization. Our results show that priming EpiSCs with the appropriate form of TGF-β signalling at the formative phase of endoderm differentiation impacts on the further progression into mature DE-derived lineages, and that this is influenced by the initial characteristics of the cell population. Our study also highlights that Activin A, which is commonly used as an in vitro surrogate for Nodal in differentiation protocols, does not elicit the same downstream effects as Nodal, and therefore may not effectively mimic events that take place in the mouse embryo.

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Sequential allocation and global pattern of movement of the definitive endoderm in the mouse embryo during gastrulation

Cited by 90 publications

References 36 publications

Dynamic expression of Thyrotropin‐releasing hormone in the mouse definitive endoderm

Dynamic expression of Thyrotropin‐releasing hormone in the mouse definitive endoderm

Expression of two novel transcripts in the mouse definitive endoderm

Differential response of epiblast stem cells to Nodal and Activin signalling: a paradigm of early endoderm development in the embryo

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