Sequential cloud-point extraction for toxicological screening analysis of medicaments in human plasma by high pressure liquid chromatography with diode array detector

Madej, Krzysztof; Persona, Karolina; Wandas, Monika; Gomółka, Ewa

doi:10.1016/j.chroma.2013.08.093

Cited by 19 publications

(8 citation statements)

References 16 publications

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“…Generally, classical STA uses a combination of multiple techniques such as immunoassays to detect substance classes (Camara et al ., ; Escobar et al ., ), gas chromatographic techniques such as headspace‐GC (HS‐GC) to identify volatile analytes (Snow and Slack, ; Schulz et al ., ) and liquid chromatographical techniques such as rapid emergency drug identification (Remedi TM ‐HS) coupled to UV detection or high‐performance liquid chromatography (HPLC) coupled to diode array detection (DAD) (Viette et al ., ; Madej et al . ) to determine nonvolatile substances. GC coupled to mass spectrometry (GC‐MS) and HPLC‐DAD techniques is routinely used for STA (Maurer et al ., ; Pragst et al ., ), but also multi‐analyte LC coupled to tandem mass spectrometry (LC‐MS/MS) and LC coupled to quadrupole time‐of‐flight tandem mass spectrometry (LC‐MS‐Qq‐TOF) based screening methods are of increasing importance in the field of the determination of pharmaceuticals in human biological samples (Dresen et al ., ; Lynch et al ., ; Broecker et al ., ; Johnson and Botch, ; Köhler et al ., ; Remane et al ., ,; Oberacher et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Toxicological screening of human plasma by on‐line SPE‐HPLC‐DAD: identification and quantification of basic drugs and metabolites

Mut

Grobosch

Binscheck-Domaß

et al. 2014

Biomedical Chromatography

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An automated multi-analyte screening method for the identification and quantification of 92 drugs and metabolites based on on-line solid-phase extraction-high-performance liquid chromatography-diode array detection technique was developed and successfully validated. In addition, a database with 870 entries including UV-spectra, relative/retention times and response factors of toxicologically relevant compounds was created. Plasma samples (0.2 mL) were treated with methanol, diluted with buffer and on-line extracted (Strata X, 20 ×2 mm, 25 µm) at pH 9. Analytical separation was carried out on a Gemini NX column (150 ×4.6 mm, 3 µm) using gradient elution with acetonitrile-water (90:10,v/v) and 0.05 m potassium dihydrogen phosphate buffer (pH 2.3). Linear calibration curves with correlation coefficients ≥0.9950 were obtained for 78 analytes. As an additional benefit, the newly developed method allows the quantification of 42 analytes (e.g. antidepressants, neuroleptics and anticonvulsants) in a concentration range suitable for therapeutic drug monitoring. Limits of quantitation ranged from 0.02 mg/L (chlordiazepoxide) to 3.4 mg/L (mexiletine). Inter- and intra-day precisions of quality control samples (low/high) were better than 15% (zolpidem) and accuracy (bias) ranged from -11% (opipramol, venlafaxine) to 11% (venlafaxine, trazodone). Tests for carry-over and sample stability under different storage conditions were also performed and stability was adequate. Four cases of poisoning analysis are presented.

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Section: Introductionmentioning

confidence: 99%

Toxicological screening of human plasma by on‐line SPE‐HPLC‐DAD: identification and quantification of basic drugs and metabolites

Mut

Grobosch

Binscheck-Domaß

et al. 2014

Biomedical Chromatography

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“…The QC sample accuracy fell within the range of 90-105%, repeatability 6.9-8.2% and reproducibility 6.5-9.2% (Table 1). Chromatograms obtained for the 9 blank plasma samples and the lowest limit of quantitation of antazoline in plasma (LLOQ, 10 ng mL −1 ) are presented in Figs. 2A and 2B, respectively.…”

Section: Precision Accuracy Calibration Curve and Carry-overmentioning

confidence: 99%

“…This phase separates into two isotropic phases containing the surfactant in different concentrations. In the surfactant micellar-rich phase, the hydrophobic organic components present in the sample are concentrated and separated from more hydrophilic compounds [9]. This method is fast, easy and does not require use of organic solvents.…”

Section: Introductionmentioning

confidence: 99%

Cloud-point extraction is compatible with liquid chromatography coupled to electrospray ionization mass spectrometry for the determination of antazoline in human plasma

Giebułtowicz

Kojro

Piotrowski

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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“…In recent years, surfactant-based coacervation extractions have gained great attention in the field of sample pretreatment because they are simple, fast, inexpensive, environmentally friendly, and with highly efficient preconcentration ability, in comparison with traditional liquid-liquid extractions [5][6][7][8][9][10]. Among them, nonionic surfactant-based coacervation extraction, also called as cloud point extraction (CPE), has been the most widely used one due to the easy formation of coacervation induced by temperature changes.…”

Section: Introductionmentioning

confidence: 99%

Hexafluoroisopropanol-induced coacervation in aqueous mixed systems of cationic and anionic surfactants for the extraction of sulfonamides in water samples

Chen

Zhang

et al. 2014

Anal Bioanal Chem

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Hexafluoroisopropanol (HFIP)-induced coacervation in aqueous mixed systems of catanionic surfactants of dodecyltrimethylammonium bromide (DTAB) and sodium dodecyl sulfate (SDS) was described in detail, and its application in the extraction of strongly polar sulfonamides (SAs) was investigated. With 10 % (v/v) HFIP inclusion, coacervation formation and two-phase separation occur in a wide range of SDS/DTAB mole ratios (88:12∼0:100 mol/mol) and total surfactant concentrations (10∼200 mmol/L). The interactions between HFIP and DTAB play an important role in coacervation formation. The HFIP-induced SDS-DTAB coacervation extraction proves to be an efficient method for the extraction and preconcentration of SAs. Both hydrophobic interaction and polar interactions (hydrogen-bond, electrostatic, and π-cation) contribute to the distribution of SAs into coacervate phase. The proposed HFIP-induced SDS-DTAB coacervation extraction combined with HPLC-UV was employed for the extraction and quantitative determination of SAs in environmental water samples. Limits of detection were 1.4∼2.5 ng mL(-1). Excellent linearity with correlation coefficients from 0.9990 to 0.9995 was obtained in the concentration of 0.01∼10 μg mL(-1). Relative recoveries were in the range of 93.4∼105.9 % for analysis of the lake, underground, and tap water samples spiked with SAs at 0.01, 1.0, and 10 μg/mL, respectively. Relative standard deviations were 0.7∼3.2 % for intraday precision and 1.3∼4.6 % for interday precision (n = 3). Concentration factors were 17∼49 for three water samples spiked with 0.01 μg/mL SAs. The results demonstrate that the proposed extraction method is feasible for the preconcentration and determination of trace SAs in real water samples.

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Sequential cloud-point extraction for toxicological screening analysis of medicaments in human plasma by high pressure liquid chromatography with diode array detector

Cited by 19 publications

References 16 publications

Toxicological screening of human plasma by on‐line SPE‐HPLC‐DAD: identification and quantification of basic drugs and metabolites

Toxicological screening of human plasma by on‐line SPE‐HPLC‐DAD: identification and quantification of basic drugs and metabolites

Cloud-point extraction is compatible with liquid chromatography coupled to electrospray ionization mass spectrometry for the determination of antazoline in human plasma

Hexafluoroisopropanol-induced coacervation in aqueous mixed systems of cationic and anionic surfactants for the extraction of sulfonamides in water samples

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