Sequential injection chromatographic determination of paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets

Abstract

In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed-phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith Flash RP-18e, 25-4.6mm column (Merck, Germany) and a FIAlab 3000 system (USA) with an 8-port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile-(0.01 M) phosphate buffer (10:90, v/v) pH 4.05, flow rate 0.6 mL min(-1). UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity (r >0.999) for all compounds, detection limits in the range 0.3-0.8 microg mL(-1), repeatability (RSD) of peak heights between runs in the range 1.10-4.30% at three concentration levels and intra-day repeatability of the retention times in the range 0.28-0.43%. The analysis time was <6 min. The method was found to be applicable for the routine analysis of the active compounds paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets.

Two-column Sequential Injection Chromatography—New approach for fast and effective analysis and its comparison with gradient elution chromatography

“…The optimization of mobile phase 1 for the first part of analysis was started with acetonitrile/water (20:80, v/v) mobile phase with help of recent published article [23]. Methanol based mobile phases were tested with less satisfactory results than acetonitrile based that showed better separation of peaks and peak symmetry.…”

Resolution and quantification challenge of modern chemometric models in the determination of anti-migraine tablets containing ergotamine, caffeine, acetaminophen, and metoclopramide

“…It acts as a stimulant of the central nervous system (CNS) and is sometimes given with ERG in formulations for the treatment of migraine. 6 Several analytical procedures have been described for the simultaneous determination of mixtures containing PAR and CAF, including spectrophotometry, 7 chemometrics, 8 highperformance thin-layer chromatography (HPTLC), 9 highperformance liquid chromatography (HPLC), [10][11][12] and gas chromatography. 13 The United States Pharmacopeia (USP) 14 describes the determination of ERG and CAF tablets by HPLC with uorimetric detection for ERG at l em ¼ 435 nm and l ex ¼ 325 nm, and ultraviolet (UV) detection for CAF at 254 nm.…”

Electrochemical Sensors for Detection of Acetylsalicylic Acid

“…A number of analytical approaches such as UV-Vis spectrometry [20], spectrofluorimetry [21-23], flow injection analysis [24-28] and high performance liquid chromatography (HPLC) [29-31] coupled with different detectors has been suggested for determination of acetylsalicylic acid. Each method has its advantages and limitations and may serve a particular need in analysis.…”