Sera of glaucoma patients show autoantibodies against myelin basic protein and complex autoantibody profiles against human optic nerve antigens

Joachim, Stephanie C.; Reichelt, Jan; Berneiser, Simone; Pfeiffer, Norbert; Grus, Franz H.

doi:10.1007/s00417-007-0737-8

Cited by 82 publications

(66 citation statements)

References 45 publications

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“…In glaucomatous patients, serum autoantibodies directed against retinal antigens have been detected, despite the absence of the lymphatic system in the retina (Dervan et al, 2010;Joachim et al, 2008;Maruyama et al, 2000;Tezel et al, 1998;Tezel et al, 1999;Yang et al, 2001b). It seems that, in glaucoma, antibodies could exit the retinal vessels and bind to target RGC.…”

Section: Mechanisms Responsible For Contralateral Eye Involvementmentioning

confidence: 95%

Macro- and microglial responses in the fellow eyes contralateral to glaucomatous eyes

Ramírez

Salazar

Hoz

et al. 2015

Progress in Brain Research

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Section: Mechanisms Responsible For Contralateral Eye Involvementmentioning

confidence: 95%

Macro- and microglial responses in the fellow eyes contralateral to glaucomatous eyes

Ramírez

Salazar

Hoz

et al. 2015

Progress in Brain Research

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“…A major risk factor is an elevated intraocular eye pressure, but the fact that 30 % of glaucoma patients fail to demonstrate this symptom [12] reveals, that other pathogenesis factors are involved, such as immunological components. Complex analysis of sera derived from numerous glaucoma patients showed up-as well as down-regulated autoantibodies against alpha-foldrin (up-regulated) or αB-Crystallin and Vimentin (down-regulated) and additionally complex changes in the antibody reactions against human optic nerve antigens [13][14][15]. Furthermore, antibody reactions in the aqueous humour of glaucoma patients against antigens such as αB-Crystallin (down-regulated), Vimentin (down-regulated) and Hsp 70 (up-regulated) [15] were detected.…”

Section: Introductionmentioning

confidence: 99%

GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57

Wilding

Bell

Funke

et al. 2015

Journal of Pharmacological Sciences

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The pathogenesis of glaucoma, a common neurodegenerative disease, involves an immunologic component. Changes in the natural autoantibody profile of glaucoma patients were detected, showing not only up-regulated but also down-regulated immunoreactivities. In recent studies we were able to demonstrate that the antibody changes have a large influence on protein profiles of neuroretinal cells. Furthermore we could demonstrate neuroprotective potential of one of the down-regulated antibodies (γ-synuclein antibody). Anti-GFAP antibody is another antibody found down-regulated in glaucoma patients. Since GFAP expression is intensified in glaucomatous retina, the aim of this study was to detect the effect of GFAP antibodies on neuroretinal cells. This is realized with a viability-test as well as proteomic analysis of cells incubated with GFAP antibodies. Furthermore, possible interaction partners of the GFAP antibody in neuroretinal cells were identified by western blot, mass spectrometry and indirect immunofluorescence staining. We found that the GFAP antibody is able to protect cells from oxidative stress, which is due to changed protein expressions of the actin cytoskeleton. Furthermore we detected a cross-reaction of the antibody to endoplasmic reticulum resident protein 57 on the cell membrane, which seems to lead to a changed signaling in the cells triggering the protective effects.

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“…Also antibodies (abs) have emerged in the research focus as potential players in GON. In several studies autoabs and complex autoab-profiles could be detected in body fluids of patients with glaucoma compared to healthy subjects [12–15]. Furthermore, not only up-regulated, but also down-regulated abs are shown in glaucoma affected individuals [16].…”

Section: Introductionmentioning

confidence: 99%

Longitudinal Analysis of Serum Autoantibody-Reactivities in Patients with Primary Open Angle Glaucoma and Optic Disc Hemorrhage

et al. 2016

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BackgroundThe aim of our current investigation was to analyze the autoantibody-reactivities of primary open angle glaucoma patients with optic disc hemorrhage as possibly correlated to disease progression by means of a protein microarray approach.MethodsSera of patients with primary open angle glaucoma and optic disc hemorrhage (n = 16) were collected directly after study inclusion (0 weeks) and after 2 weeks, 4 weeks and 12 weeks. As a control group patients with primary open angle glaucoma (n = 18) were used (0 weeks and 12 weeks). Microarrays were incubated and occurring antibody-antigen-reactions were visualized with fluorescence labeled anti-human-IgG secondary antibodies. To detect changes in autoantibodies spot intensities were digitized and compared.ResultsWith respect to the immunoreactivity at 0 weeks level increment of anti-adaptor protein 1 complex subunit mu-1 antibodies and anti-SPRY domain-containing SOCS box protein 3 antibodies in sera of primary open angle patients with optic disc hemorrhage was detected. Linear trend analysis revealed a positive correlation with r ≥ 0.8 between antibody-level and time course. Control group show no relevant changes in the same period. Significant changes were found in time point 4 comparison between patient groups in anti-adaptor protein 1 complex subunit mu-1-level (p = 0.01). No significant changes in visual acuity were found.ConclusionWith this approach we were able to detect autoimmune reactivities in sera of patients with primary open angle glaucoma and optic disc hemorrhage compared to patients without optic disc hemorrhage. These antibodies could give further insights into the pathogenesis and the autoimmune component of glaucomatous optic neuropathy.

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Sera of glaucoma patients show autoantibodies against myelin basic protein and complex autoantibody profiles against human optic nerve antigens

Cited by 82 publications

References 45 publications

Macro- and microglial responses in the fellow eyes contralateral to glaucomatous eyes

Macro- and microglial responses in the fellow eyes contralateral to glaucomatous eyes

GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57

Longitudinal Analysis of Serum Autoantibody-Reactivities in Patients with Primary Open Angle Glaucoma and Optic Disc Hemorrhage

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