Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue

Abstract

Serglycin is a proteoglycan highly expressed by immune cells, in which its functions are linked to storage, secretion, transport, and protection of chemokines, proteases, histamine, growth factors, and other bioactive molecules. In recent years, it has been demonstrated that serglycin is also expressed by several other cell types, such as endothelial cells, muscle cells, and multiple types of cancer cells. Here, we show that serglycin expression is upregulated in transforming growth factor beta (TGF-β) induced epithelial-mesenchymal transition (EMT). Functional studies provide evidence that serglycin plays an important role in the regulation of the transition between the epithelial and mesenchymal phenotypes, and it is a significant EMT marker gene. We further find that serglycin is more expressed by breast cancer cell lines with a mesenchymal phenotype as well as the basal-like subtype of breast cancers. By examining immune staining and single cell sequencing data of breast cancer tissue, we show that serglycin is highly expressed by infiltrating immune cells in breast tumor tissue.

Cohort profile: The Clinical and Multi-omic (CAMO) cohort, part of the Norwegian Women and Cancer (NOWAC) study

“…TMAs are used for high throughput visualization of molecular targets on DNA-, miRNA-, mRNA- or protein level, using HE staining, IHC, and in situ hybridization (ISH). Assessment of a wide range of potential biomarkers using TMAs is planned or currently ongoing [ 15 ].…”

Effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathway

“…Although not presented in the Results section, serum SRGN and PF4 levels showed a high correlation with ELISA (ρ = 0.813, p < 0.001). SRGN has been reported to be involved in the regulation of epithelial-mesenchymal transitions (EMTs) via the TGF-β signaling pathway in breast cancer tissues [34]. In addition, HCC patients with a high SRGN expression exhibit a significantly shorter overall survival and higher recurrence rates than those with a low SRGN expression [35].…”

Matrisomal components involved in regenerative wound healing in axolotl and Acomys: implications for biomaterial development

“…Moreover, serglycin is upregulated under the influence of TGF-β1 during epithelial-mesenchymal transition, this transition is an important process during wound healing. 152 Serglycin is densely packed with various GAGs, leading to a high density of GAG-binding proteins, and one of these associated GAGs is heparin. 151 Heparin is most commonly associated with anticoagulation and anti-inflammation and has widespread use in therapeutic applications.…”