Serial Implantations of Larval Schistosoma mansoni from Infected to Uninfected Snails

Nojima, Hisatake; Noda, Shinichi; Sato, Atsuo

doi:10.2307/3280751

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…Perhaps the technique's greatest benefit has been the perpetual maintenance of schistosome clones Nojima et al 1980;Cohen and Eveland 1984;Jourdane 1984Jourdane , 1990. In addition to circumventing the requirement for a vertebrate-host (Jourdane 1990), schistosome clones obtained by this technique can be maintained and biologically characterized (Cohen and Eveland 1988).…”

Section: Transplantation Of Parthenitaementioning

confidence: 99%

“…This balanced salt solution has been used with various combinations of antibiotics (Chernin 1963;Cohen and Eveland 1984;Jourdane et al 1985). Diluted Schneider's Drosophila medium (110 mOsm/Kg H 2 O) was used in one study (Nojima et al 1980). Parasitized tissues (hepatopancreas and ovotestis) are excised and cut into 1-2 mm 3 fragments using iris scissors (Chernin 1966;Cohen 1984).…”

Section: Transplantation Of Parthenitaementioning

confidence: 99%

“…While most investigators have implanted donor snail fragments into the exposed cephalopedal sinus of anesthetized recipient snails, sporocysts have also been implanted into the cephalopedal sinuses of nonanesthetized snails via a 1.5-mm hole in the shell (Nojima et al 1980). A later study found success of transplantation (determined by resultant patent infections) to be greater in anesthetized snails with exposed cephalopedal sinuses than in nonanesthetized snails implanted through a hole in the shell (Cohen and Eveland 1984).…”

Section: Transplantation Of Parthenitaementioning

confidence: 99%

“…A later study found success of transplantation (determined by resultant patent infections) to be greater in anesthetized snails with exposed cephalopedal sinuses than in nonanesthetized snails implanted through a hole in the shell (Cohen and Eveland 1984). Holes in snail shells were sealed with "Dental Cyanon" (a-cyanoacrylate) (Nojima et al 1980) or with plasticene (Cohen and Eveland 1984). In addition, implantation of sporocysts developed in vitro in the (Ivanchenko et al 1999;Kapp et al 2003).…”

Section: Transplantation Of Parthenitaementioning

confidence: 99%

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Laboratory Rearing of Biomphalaria glabrata Snails and Maintenance of Larval Schistosomes In Vivo and In Vitro

Eveland

Haseeb²

2010

Biomphalaria Snails and Larval Trematodes

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This chapter describes the methods used by the current authors and numerous other investigators to optimize conditions for successfully growing and maintaining both uninfected and infected Biomphalaria glabrata in the laboratory, with particular emphasis on the optimal physical, chemical, and biological factors that affect successful snail rearing and/or breeding, and the ways that they can be measured. Consideration is also given to incidental organisms that can alter aquarium ecology and affect both the snails and their intended trematode parasites. Various methods of infecting snails and maintaining snail tissues and cells in vitro are also described. The goal of this chapter is to aid researchers who have the need or desire to maintain the life cycle of this pulmonate and its trematode parasites in the laboratory.

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Section: Transplantation Of Parthenitaementioning

confidence: 99%

Section: Transplantation Of Parthenitaementioning

confidence: 99%

Section: Transplantation Of Parthenitaementioning

confidence: 99%

Section: Transplantation Of Parthenitaementioning

confidence: 99%

See 2 more Smart Citations

Laboratory Rearing of Biomphalaria glabrata Snails and Maintenance of Larval Schistosomes In Vivo and In Vitro

Eveland

Haseeb²

2010

Biomphalaria Snails and Larval Trematodes

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Biomphalaria Snails and Larval Trematodes

Toledo

Fried

2011

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), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights.Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) v The history of research on the relationships between Biomphalaria spp. and larval trematodes, particularly Schistosoma mansoni, is long, active, and ongoing today. Snails of the genus Biomphalaria are of significant medical importance with many species living in freshwater habitats associated with human settlements; many of these snail species are obligate intermediate hosts of the human blood fluke S. mansoni, the causative agent of hepatosplenic schistosomiasis. This parasitic disease continues to disrupt the lives of about 200 million people in over 70 countries and prevents these individuals from otherwise reasonable expectations of healthy and productive lives. Furthermore, it is estimated that within the developing world, especially in sub-Saharan Africa, more than 700 million people are at risk of becoming infected, despite the efforts to control transmission in human and snail populations by mass chemotherapy and the use of molluscicides. Several factors, such as the absence of a schistosome vaccine, the recent appearance of resistance to antischistosome drugs, and human activities that expand snail habitats, have increased the need for a better understanding of schistosome-snail interactions. In recent years, the application of new technologies has contributed to the accumulation of considerable new information on this topic that may be of great use to biomedical scientists.In addition to the impact of Biomphalaria spp. on public health, these snails are also interesting models for the study of other topics such as population biology, including genetics and demography, proteomics, invertebrate immunobiology, mating systems, and biogeography, among others. Biomphalaria spp. snails have been extensively used as experimental biological models contributing significantly to new developments in many areas studied by biomedical scientists. Extensive coverage of these topics is included in this book, also considering trematode species other than schistosomes.The aim of the present book is to provide an overview of the recent advances in the Biomphalaria spp.-larval trematode interactions, especially in Biomphalariaschistosome systems. Emphasis is placed on gaps in our knowledge that must be filled to gain a better understanding of the relationships in these host-parasite systems. This may be critical for a deeper knowledge of the transmission of schistosomiasis and other snail-borne parasitic diseases. Pref...

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Schistosoma mansoni: Long-term maintenance of clones by microsurgical transplantation of sporocysts

Cohen

Eveland

1984

Experimental Parasitology

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Serial Implantations of Larval Schistosoma mansoni from Infected to Uninfected Snails

Cited by 7 publications

References 11 publications

Laboratory Rearing of Biomphalaria glabrata Snails and Maintenance of Larval Schistosomes In Vivo and In Vitro

Laboratory Rearing of Biomphalaria glabrata Snails and Maintenance of Larval Schistosomes In Vivo and In Vitro

Biomphalaria Snails and Larval Trematodes

Schistosoma mansoni: Long-term maintenance of clones by microsurgical transplantation of sporocysts

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