Serine Protease Inhibitors Expressed in the Process of Budding of Tunicates as Revealed by EST Analysis

Kawamura, Kazuo; Hayata, Daichika; Fujiwara, Shigeki; Yubisui, Toshitsugu

doi:10.1093/oxfordjournals.jbchem.a022192

Cited by 12 publications

(13 citation statements)

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“…1996). It is associated with a serine protease inhibitor, P‐serpin that has been identified previously as budding‐specific cDNA (Kawamura et al . 1998).…”

Section: Dedifferentiation‐regulating Factors In Colonial Tunicatesmentioning

confidence: 99%

Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates

Kawamura¹,

Sugino

Sunanaga

et al. 2007

Dev Growth Differ

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The cellular and molecular features of multipotent epithelial cells during regeneration and asexual reproduction in colonial tunicates are described in the present study. The epicardium has been regarded as the endodermal tissue-forming epithelium in the order Enterogona, because only body fragments having the epicardium exhibit the regenerative potential. Epicardial cells in Polycitor proliferus have two peculiar features; they always accompany coelomic undifferentiated cells, and they contain various kinds of organelles in the cytoplasm. During strobilation a large amount of organelles are discarded in the lumen, and then, each tissue-forming cell takes an undifferentiated configuration. Septum cells in the stolon are also multipotent in Enterogona. Free cells with a similar configuration to the septum inhabit the hemocoel. They may provide a pool for epithelial septum cells. At the distal tip of the stolon, septum cells are columnar in shape and apparently undifferentiated. They are the precursor of the stolonial bud. In Pleurogona, the atrial epithelium of endodermal origin is multipotent. In Polyandrocarpa misakiensis, it consists of pigmented squamous cells. The cells have ultrastructurally fine granules in the cytoplasm. During budding, coelomic cells with similar morphology become associated with the atrial epithelium. Then, cells of organ placodes undergo dedifferentiation, enter a cell division cycle, and commence morphogenesis. Retinoic acid-related molecules are involved in this dedifferentiation process of multipotent cells. We conclude that in colonial tunicates two systems support the flexibility of tissue remodeling during regeneration and asexual reproduction; dedifferentiation of epithelial cells and epithelial transformation of coelomic free cells.

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“…1996). It is associated with a serine protease inhibitor, P‐serpin that has been identified previously as budding‐specific cDNA (Kawamura et al . 1998).…”

Section: Dedifferentiation‐regulating Factors In Colonial Tunicatesmentioning

confidence: 99%

Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates

Kawamura¹,

Sugino

Sunanaga

et al. 2007

Dev Growth Differ

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“…With regard to P‐trefoil , the previous EST analysis did not hit any trefoil factors (Kawamura et al . 1998).…”

Section: Discussionmentioning

confidence: 95%

“…We speculated whether P‐Serpin binds to serine protease(s) since P‐Serpin can interact with trypsin and elastase and inhibit their enzymatic activities (Kawamura et al . 1998).…”

Section: Discussionmentioning

confidence: 99%

“…This was added to the solution mentioned above in a final concentration of 0.1 m m . Proteolytic digestion and subsequent measurement of the activity were performed, as described elsewhere (Kawamura et al . 1998; Ohashi et al .…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we constructed a catalog of genes that are expressed during budding in P. misakiensis (Kawamura et al . 1998).…”

Section: Introductionmentioning

confidence: 99%

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Molecular collaborations between serpins and trefoil factor promote endodermal cell growth and gastrointestinal differentiation in budding tunicates

et al. 2006

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We present evidence supporting novel collaborations between the serine protease inhibitor (serpin) and the trefoil factor during the budding stage of the tunicate Polyandrocarpa misakiensis . Using a maltose-binding protein/P-serpin fusion protein, two polypeptides of 40 kDa and 45 kDa were pulled down from Polyandrocarpa homogenates. Based on their partial amino acid sequence data, a single cDNA (928 bp) was cloned. It encodes a polypeptide that has five tandem repeats of a trefoil consensus motif. Thus, we termed the cDNA P-trefoil . Both P-trefoil and P-serpin were expressed exclusively by coelomic cells during budding. P-Trefoil was expressed mainly by coelomic cells throughout the asexual life cycle of Polyandrocarpa , while P-Serpin was localized particularly in coelomic cells and in the extracellular matrix in developing buds. The native PTrefoil protein showed aminopeptidase activity. It induced cell growth in cultured Polyandrocarpa cells at a concentration of 8 µ g/mL. P-Serpin reinforced this activity of P-Trefoil. Further, a mixture of P-Trefoil and P-Serpin exhibited the in vitro induction of a gut-specific alkaline phosphatase. These results show for the first time that a serpin can interact with a trefoil factor to play a role in the cellular growth and differentiation of the gastric epithelium.

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Identification of cDNAs associated with late dedifferentiation in adult newt forelimb regeneration

Vascotto

Beug

Liversage

et al. 2005

Developmental Dynamics

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Epimorphic limb regeneration in the adult newt involves the dedifferentiation of differentiated cells to yield a pluripotent blastemal cell. These mesenchymal-like cells proliferate and subsequently respond to patterning and differentiation cues to form a new limb. Understanding the dedifferentiation process requires the selective identification of dedifferentiating cells within the heterogeneous population of cells in the regenerate. In this study, representational differences analysis was used to produce an enriched population of dedifferentiation-associated cDNA fragments. Fifty-nine unique cDNA fragments were identified, sequenced, and analyzed using bioinformatics tools and databases. Some of these clones demonstrate significant similarity to known genes in other species. Other clones can be linked by homology to pathways previously implicated in the dedifferentiation process. These data will form the basis for further analyses to elucidate the role of candidate genes in the dedifferentiation process during newt forelimb regeneration. Developmental Dynamics 233:347-355, 2005.

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Serine Protease Inhibitors Expressed in the Process of Budding of Tunicates as Revealed by EST Analysis

Cited by 12 publications

References 0 publications

Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates

Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates

Molecular collaborations between serpins and trefoil factor promote endodermal cell growth and gastrointestinal differentiation in budding tunicates

Identification of cDNAs associated with late dedifferentiation in adult newt forelimb regeneration

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