1976
DOI: 10.1016/0003-9861(76)90349-0
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Serine transhydroxymethylase: Mechanism of aldolase activation by folate

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Cited by 25 publications
(6 citation statements)
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“…The isolation procedure of Jones & Priest (1976) was modified for the isolation of large quantities of serine hydroxymethyltransferase from bovine liver. All the buffers used contained 10 mM-2-mercaptoethanol and I mM-EDTA.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The isolation procedure of Jones & Priest (1976) was modified for the isolation of large quantities of serine hydroxymethyltransferase from bovine liver. All the buffers used contained 10 mM-2-mercaptoethanol and I mM-EDTA.…”
Section: Methodsmentioning
confidence: 99%
“…This value is similar to the value of approx. 50000 obtained by Jones & Priest (1976). The enzyme was assayed with L-allothreonine as substrate as described by Malkin & Greenberg (1964), and the pure enzyme when analysed in this way had a specific activity of 2.6 ,umol/mg of protein per min.…”
Section: Methodsmentioning
confidence: 99%
“…The special features of the present method for purification of the monkey liver enzyme were the use of a Blue Sepharose affinity matrix and the omission of thermal denaturation in the presence of L-serine, which is a major step used by earlier workers for the purification of this enzyme (Nakano et al, 1968;Fujioka, 1969;Akhtar & El-Obeid, 1972;Cheng & Haslam, 1972;Rowe & Lewis, 1973;Palekar et al, 1973;Jones & Priest, 1976;Ulevitch & Kallen, 1977). The allosteric effects of nicotinamide nucleotides on the monkey liver enzyme (Ramesh & Appaji Rao, 1978) suggested that the Blue Sepharose affinity matrix could be effectively used in the purification of the serine hydroxymethyltransferase.…”
Section: Discussionmentioning
confidence: 99%
“…Serine hydroxymethyltransferase has been isolated from several mammalian and microbial sources (Nakano et al, 1968;Fujioka, 1969;Schirch, 1971;Jones & Priest, 1976;Ulevitch & Kallen, 1977;Mansouri et al, 1972). None of these preparations showed any co-operative interactions with substrates or other metabolites, whereas the partially purified enzyme from pig kidney and monkey liver exhibited both homotropic and heterotropic interactions (Harish Kumar et al, 1976;Ramesh & Appaji Rao, 1978).…”
Section: Discussionmentioning
confidence: 99%
“…In eukaryotic cells both mitochondrial and cytosolic isoforms of the enzyme exist. Both isoforms have been purified from rabbit and rat liver (Nakano et al, 1968;Schirch & Peterson, 1980) and the cytosolic form alone from bovine and sheep liver (Jones & Priest, 1976;Ulevitch & Kallen, 1977), and from plants and bacteria (Fujioka, 1969;Rao & Rao, 1982). The cytosolic and mitochondrial enzymes have similar substrate specificities and kinetic constants (Schirch & Peterson, 1980), and the precise cellular role of each isoenzyme form is not yet known.…”
Section: Introductionmentioning
confidence: 99%