Sero Diagnosis of Dengue Virus Infection Using Elisa in Patients With Suspected Dengue Infection

Abstract

AIM:To identify the Seropositivity of dengue virus infection in clinically suspected patients. MATERIAL AND METHODS: A cross sectional study was conducted in north Karnataka from Jan 2012 to August 2013. Clinically suspected patient suffering from dengue virus infection were included in the study according to WHO criteria. Acute phase sera were collected after 5 days of the onset of fever as per WHO criteria. Serology was performed using ELISA for detection of IgG and IgM. A primary dengue infection was defined by a positive IgM ELISA and negative IgG. Secondary infection was defined as positive IgG with or without IgM. RESULTS: Among 580 patient who met the WHO criteria for dengue infection 246(42.41 %) were ELISA positive among whom 71(28.87%) had primary infection. Secondary infection was reported in 175(71.13). Acute phase sera of 344 (59.31%) were negative by ELISA. Thrombocytopenia was evident in 52(71.83%) with primary infection and 115(65.71%) patients with secondary dengue infection out of 167 patients who were positive for dengue IgM/IgG antibodies.

Dengue Infection - prevalence and seasonal variation among patients attending a tertiary care hospital at Chamarajanagar, Karnataka

“…40%. [23][24][25] This difference in seroprevalence could be due to differences in study settings, differences in study periods, differences in sampling techniques, data collection tools as well as differences in sample processing procedure/ techniques.…”