Sero-diagnosis of Dengue virus in Different Hospitals of Nepal

Abstract

INTRODUCTION: Dengue fever (DF) is an emerging mosquito borne viral disease and important public health problem in low land Terai region which is also moving towards hilly region Nepal. This study was designed to determine the sero-prevalence of dengue virus infection in patients visiting hospitals of Nepal. MATERIALS AND METHODS: This study was conducted during period (June-November) of 2010 in Nepalese patients with fever visiting hospitals of Birganj, Damouli, Biratanagar, Dhading Besi and Chitwan. The sero-prevalence of dengue virus specific IgM was determined by enzyme linked immunosorbent assay (ELISA). Serum samples were collected from 289 patients visiting hospitals with history of fever and clinically suspected dengue fever. RESULTS: The anti-dengue IgM positivity was found to be 8.99%. The positive dengue cases were higher in male (10.8%) as compared to female (7.1%) though it was not statistically significant (P>0.05). Among different age groups, the highest positive cases (12.3%) were from age group below 15 years followed by above 50 years 8.3%. Out of 5 hospitals, the highest positive cases were in Tanahu hospital, Damouli (23.8%) followed by Bharatpur hospital and Chitwan (22.2%). Age and gender were found to be independent predictors. The highest numbers of dengue positive cases were in occupation group business (13.3%) followed by agriculture (12.7%). CONCLUSIONS: Prevalence of dengue virus infection is increasing and proper control measure should be provided. IgM capture ELISA was used for laboratory analysis and remains as a reliable and inexpensive method for the diagnosis of dengue. Hence, the IgM capture ELISA has become the most accepted technique for the diagnosis of dengue in developing countries like Nepal. DOI: http://dx.doi.org/10.3126/ijim.v1i2.7003 Int J Infect Microbiol 2012;1(1):58-62

Detection of IgM against Dengue Virus in Clinically Suspected Patients Presenting at a Tertiary Care Centre, Narayani Zone, Nepal

“…In this study, 8.5% of patients were serologically positive for DVI. The positivity rate concurs with observations of 10.4% and 9.0% from Nepal in 2001 and 2012, respectively [16,17], and 13.7% from Bangladesh in 2002 [18]. While a rate of 19.7% has been reported from India in 2011 [19], this higher prevalence rate may be due to its endemic nature in that country.…”

“…A higher prevalence rate of anti-dengue IgM antibody in males has been reported from Nepal [17,20] and from India [19,23]. Herein, the prevalence of dengue-positive cases was also biased towards males over females by a factor of 1.6:1 (P<0.05).…”

Serological and Entomological Study of Dengue in Dang and Chitwan Districts of Nepal

“…The positivity for anti-dengue IgM reported by Sah et al was 30% [ 18 ] and that reported by Gupta et al was 29.09% [ 12 ]. Similarly, Poudel et al [ 19 ] and Shah et al [ 20 ] reported the positivity for anti-dengue IgM to be 12.17% and 8.99%, respectively. The reason for difference in positivity may be due to variation in the geography and effectiveness of the vector control programs in the epidemic regions as well as the demographic and social changes, urbanization and environmental changes.…”

Epidemiological scenario of dengue in the state of Manipur during the last 3 years

“…In this study 16.10% cases were dengue positive serologically which is lower than the findings of others (Singla et al, 2016;Garg et al, 2011;Chitkara et al, 2018). But the positivity rate of study is found to higher as compared to report of other studies (Sherchand et al, 2001;Shah et al, 2012;Bin Yunus et al, 2002). Such variation in seropositive rate could be due to different geographical areas and climatic conditions (Kashinkunti & Shiddappa, 2013).…”