Serodiagnosis of Fasciola gigantica Infection in Buffaloes with Native Cathepsin-L Proteases and Recombinant Cathepsin L1-D

Aftab, Andleeb; Lall, Rohit; Bisen, Savita; Arun, A. B.; Rialch, Ajayta; Chamuah, J. K.; Yadav, Shobha; Silamparasan, M.; Raina, O.K.

doi:10.2478/s11686-020-00177-3

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…Fasciola cat-B proteins are also secreted like cat-Ls, which are immunogenic and easily exploited in diagnostic assays of Fasciola. In fact, both cat-L and cat-B proteins can be used in combination in an immunodiagnostic assay for enhancing the detection sensitivity of prepatent and patent infections (Aftab et al 2020). Recently, Jacob et al (2023) reported a first effort to use recombinant cat B-5 antigen to develop ELISA that showed 95.3 % sensitivity and 92.4 % specificity (with a cut-off of 60% positive) in the serosurveillance of bovine tropical fasciolosis in India.…”

Section: Diagnostic Approaches For Fasciola Infection In Livestockmentioning

confidence: 99%

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Advances in diagnostic approaches to Fasciola infection in animals and humans: An overviews

Aftab,

Raina,

Maxton

et al. 2024

J. Helminthol.

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Fasciolosis, caused by Fasciola hepatica and F. gigantica, is an impediment to the livestock industry’s expansion and has a massively negative socio-economic impact due to its widespread prevalence in livestock. It is a waterborne zoonosis affecting human populations in the countries where rural economies are associated with livestock rearing. Conventional diagnosis of Fasciola infection is done by detecting parasite eggs in the faeces of infected animals or by immunological methods. Accurate and quick immunodiagnosis of Fasciola infection in animals and humans is based on the detection of antibodies and specific antigens expressed in the prepatent stage of the parasite. Both molecular and serodiagnostic tests developed thus far have enhanced the reliability of Fasciola diagnosis in both man and animals but are not widely available in resource-poor nations. A pen-side diagnostic test based on a lateral flow assay or a DNA test like loop-mediated isothermal amplification (LAMP) would be simple, fast, and cost-effective, enabling clinicians to treat animals in a targeted manner and avoid the development of drug resistance to the limited flukicides. This review focuses on the recent advances made in the diagnosis of this parasite infection in animals and humans.

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Section: Diagnostic Approaches For Fasciola Infection In Livestockmentioning

confidence: 99%

“…In fact, both cat-L and cat-B proteins can be used in combination in an immunodiagnostic assay for enhancing the detection sensitivity of prepatent and patent infections (Aftab et al . 2020). Recently, Jacob et al .…”

Section: Diagnostic Approaches For Fasciola Infection In Livestockmentioning

confidence: 99%

Advances in diagnostic approaches to Fasciola infection in animals and humans: An overviews

Aftab,

Raina,

Maxton

et al. 2024

J. Helminthol.

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“…This result is the first result which proved that F. hepatica cathepsins were of N-glycosylated. Thses addresses the importance of these observations for the production of immunological studies and vaccines, in which the native members of cathepsin FhCL1, FhCL2, and FhCL3 were immunodiagnostic candidates for diagnosis of fascioliasis in sheep with 100% sensitivity (Martínez-Sernández et al, 2018), in buffaloes with 85.2% sensitivity and 86.7% specificity (Aftab et al, 2020), and in goat with 70% sensitivity (Domingo et al, 2018). Overall, these efforts pointed to the role of the glycans and its glycoconjugates antigens in the diagnosis of fascioliasis.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Platyhelminths Glycoconjugates in Diagnosis and Immune Response of Farm Animals

Shanawany¹

2021

AAVS

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| Platyhelminths (Flatworm: Neodermata) are a highly varied group of parasites containing several veterinary, biomedical and economic importance. Glycans of platyhelminths are expressed in a varity of carrier molecules including O-and N-linked glycoproteins and glycolipid structures. Their glycans exist in large quantities in the excretory/secretory products and on the parasites surface, these glycans play role in host-parasite interplay. Glycan portions of complex molecules of platyhelminths interact with antigen-presenting cells of the innate immune system, such as interact with specific receptors on dendritic cells leading to modulate host toward T helper 2 immune response for helping parasite survival. The immunomodulatory properties of platyhelminths glycans could be used to treat autoimmune and inflammatory diseases. The adaptive immune response against them characterized by the production of IgG, IgE, IL-4, IL-5, and IL-13. Anti-glycan antibodies are a common characteristic in platyhelminths infection, detection of these antibodies is widely used for serodiagnosis of platyhelminths infection. The Platyhelminthes glycoproteins and glycolipids are considered immunodiagnostic candidates for serodiagnosis of such infection in different farm animals. The specificity and sensitivity of diagnostic platyhelminths glycans are depending on the structural characterization of these glycans. Hence, the aim of this review will revolve around the application of these glycans for diagnosis of infectious diseases in farm animals. Understand their role in shaping and modulating innate and adaptive host immune responses. Immune modulation of host immune response by these glycans will make these molecules show promise in the fields of diagnosis, treatment of inflammatory and auto immune diseases, and vaccinology.

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