Serodiagnosis of Imported Schistosomiasis by a Combination of a Commercial Indirect Hemagglutination Test withSchistosoma mansoniAdult Worm Antigens and an Enzyme-Linked Immunosorbent Assay withS. mansoniEgg Antigens

Evaluation of microscopical and serological techniques in the diagnosis of Schistosoma mansoni infection at Sennar State, Central Sudan

“…This has become obvious in our finding, when we combined the both results of IHA and ELISA the specificity was increased to 85.8%. Gool et al reported that the combined use of these two tests enabled the serological diagnosis of schistosomiasis to be achieved with very high degrees of both sensitivity and specificity [10] .…”

“…The test method of ELISA was carried out for detection of S. mansoni IgG-class antibodies as previously described [10] . ELISA reagent kits (Nova Tec Immundiagnostica GmbH Technologic & Waldpark Company, Dietzenbach, Germany) were prepared according to the manufacturer's instructions.…”

“…Immunological diagnosis of S. mansoni 2.6.1. IHA method IHA assay was performed for the detection of S. mansoni antibodies using erythrocytes coated with specific adult worm antigen as described by Gool et al [10] . The IHA test kit (Fumouze Diagnostics Company, Paris, France) was prepared following the manufacturer's instructions.…”

“…Various serodiagnostic methods have been developed to detect anti-schistosomal antibodies, such as indirect hemagglutination assays (IHA), and enzyme-linked immunosorbent assays (ELISA) using different type of antigens [10,11] . In Sudan, the high endemicity of S. mansoni infections have been reported in many parts of the country [12][13][14] .…”

Schistosomiasis - An Unusual Cause of Ureteral Obstruction: A Case History and Perspective

“…[82][83][84][85][86][87][88] Falcon assay screening test (FAST)-ELISA and immunoblot assays for specific antibodies to S. mansoni and S. haematobium adult worm microsomal antigens are highly specific for both species. 89,90 However, positive results in antibody assays do not necessarily correlate with the worm burden, as measured by egg output. Also, it is not possible to distinguish previous exposure from current infections or reinfections.…”