Seroepidemiological study of human parvovirus B19 among human immunodeficiency virus-infected patients in a medium-sized city in Rio de Janeiro, Brazil

Azevedo, Kátia Martins Lopes de; Setúbal, Sérgio; Camacho, L; Velarde, Luís Guillermo Coca; Oliveira, Solange Artimos de

doi:10.1590/s0074-02762009000600014

Cited by 14 publications

(11 citation statements)

References 15 publications

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“…In this study, evaluating the seroprevalence and B19V DNA positivity among blood donors in the Southern part of Brazil we found 53.9% B19V IgG seroprevalence and 1.9% positivity of B19V DNA. The detected seroprevalence was similar to the seroprevalences obtained for blood donors in other Brazilian regions like the São Paulo state (60%), and in HIV‐positive individuals (62%) from Rio de Janeiro . It was also observed that the seroprevalence increased over the age (ie, 42.8% in blood donors of 17‐29 years to 65.5% in donors of >40 years).…”

Section: Discussionsupporting

confidence: 82%

Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil

Slavov

Rodrigues

Sauvage

et al. 2019

Journal of Medical Virology

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Usually transmitted via respiratory droplets, parvovirus B19 (B19V) can also be acquired by blood transfusion especially because of viral persistence, resistance to blood treatment procedures, and high viral load during the early infection phase. This is particularly problematic in immunocompromised or anemic patients where the infection can have a severe outcome. As B19V DNA was detected in blood donations from South Brazil during a viral metagenomic survey performed by Next-Generation Sequencing, the objective of this retrospective study was to evaluate the seroprevalence, B19V DNA presence and circulating genotypes in a Hospital Blood Transfusion Service in Santa Maria city in South Brazil (Rio Grande do Sul state). Among 480 volunteer blood donors, 53.9% (n = 258 of 479) were anti-B19V IgGpositive, and 9 (1.9%) plasma samples presented B19V DNA. In almost all cases (n = 7 of 9, 77.8%), B19V DNA load was accompanied by the presence of anti-B19V IgG suggesting a persistent infection. The sequencing of the strains demonstrated that all belong to genotype 1 which is the most prevalent worldwide. The analysis of the recipient information of the positive for B19V DNA units revealed no related posttransfusion adverse effects. Our results demonstrate for the first time, B19V seroprevalence, viral load, and genotypes among blood donors from South Brazil and give a light for the circulation and impact of this B19V in this part of the country. K E Y W O R D S blood donors, genotypes, parvovirus B19, real-time polymerase chain reaction, seroprevalence J Med Virol. 2019;91:1224-1231. wileyonlinelibrary.com/journal/jmv 1224 |

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Section: Discussionsupporting

confidence: 82%

Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil

Slavov

Rodrigues

Sauvage

et al. 2019

Journal of Medical Virology

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“…However, with the introduction of highly active antiretroviral treatment (HAART), recent studies have shown low number of cases with anemia caused by B19 in HIV infected patients [de Azevedo et al, ; Pereira et al, ]. Several studies have reported that almost 30% of HIV infected patients have no IgG antibodies against B19 and are at risk of developing B19 infection, persistent viremia and chronic anemia [Azevedo et al, ]. B19 IgG negative patients are at risk for primary infection whereas IgG positive immunocompromised patients may be considered at risk of both reinfection with another genotype or reactivation of a B19 persistent infection according to few studies [Weiland et al, ; Bremner et al, ].…”

Section: Introductionmentioning

confidence: 99%

Frequency and genotype of human parvovirus B19 among Iranian patients infected with HIV

et al. 2015

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The human parvovirus B19 (B19) usually causes a subclinical infection in immunocompetent individuals. Whereas immunocompromised individuals such as patients infected with HIV are at risk of persistent anemia due to B19 infection. Only few studies have been carried out on distribution and molecular epidemiology of B19 in Iran. We aimed to determine the frequency and genotype of B19 among Iranian patients infected with HIV. We conducted a survey on 99 HIV patients and 64 healthy controls. IgG and IgM antibodies against B19 were detected by ELISA and B19 DNA was assessed by nested PCR. PCR products were subjected to direct sequencing and classified after phylogenetic analysis. The prevalence of B19 immunoglobulin was 11.1% for IgG and 1% for IgM. B19 DNA was detected in 13.1% of cases. The prevalence of B19 IgG, IgM, and DNA in control group was 25%, 1.6%, and 9.4%, respectively. B19 IgG was significantly lower in HIV group than in normal controls. There was no significant difference regarding anemia between cases and controls. All sequenced B19 isolates belonged to genotype 1A with low genetic diversity. Our findings indicated that in the HAART era, the importance of B19 infections in HIV patients may be limited whereas persistent B19 viremia in the circulation of healthy controls raises a potential concern in blood donations.

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“…To better understand the importance of B19V infection in the HAART era, a study to estimate the frequency of B19V seroconversion in a cohort of HIV-infected patients was conducted by our group during an eight-year period (2001-2008) at the Infectious Diseases Department of Antonio Pedro University Hospital (HUAP) at the Fluminense Federal University (Niterói, state of Rio de Janeiro, Brazil) (de Azevedo et al 2009). Seroconversions were detected in approximately 30% (28 of 88) of our anti-B19 IgG-negative patients, a similar proportion to that found by others (Chernak et al 1995, Mylonakis et al 1999).…”

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Clinical features and laboratory findings of human parvovirus B19 in human immunodeficiency virus-infected patients

Pereira

Garcia

Azevedo

et al. 2014

Mem. Inst. Oswaldo Cruz

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Immunocompromised patients may develop severe chronic anaemia when infected by human parvovirus B19 (B19V). However, this is not the case in human immunodeficiency virus (HIV)-infected patients with good adherence to highly active antiretroviral treatment (HAART). In this study, we investigated the clinical evolution of five HIV-infected patients receiving HAART who had B19V infections confirmed by serum polymerase chain reaction. Four of the patients were infected with genotype 1a strains and the remaining patient was infected with a genotype 3b strain. Anaemia was detected in three of the patients, but all patients recovered without requiring immunoglobulin and/or blood transfusions. In all cases, the attending physicians did not suspect the B19V infections. There was no apparent relationship between the infecting genotype and the clinical course. In the HAART era, B19V infections in HIV-positive patients may be limited, subtle or unapparent.

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Seroepidemiological study of human parvovirus B19 among human immunodeficiency virus-infected patients in a medium-sized city in Rio de Janeiro, Brazil

Abstract: Parvovirus B19 (B-19)

Cited by 14 publications

References 15 publications

Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil

Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil

Frequency and genotype of human parvovirus B19 among Iranian patients infected with HIV

Clinical features and laboratory findings of human parvovirus B19 in human immunodeficiency virus-infected patients

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