Frequency and genotype of human parvovirus B19 among Iranian patients infected with HIV

Abstract

The human parvovirus B19 (B19) usually causes a subclinical infection in immunocompetent individuals. Whereas immunocompromised individuals such as patients infected with HIV are at risk of persistent anemia due to B19 infection. Only few studies have been carried out on distribution and molecular epidemiology of B19 in Iran. We aimed to determine the frequency and genotype of B19 among Iranian patients infected with HIV. We conducted a survey on 99 HIV patients and 64 healthy controls. IgG and IgM antibodies against B19 were detected by ELISA and B19 DNA was assessed by nested PCR. PCR products were subjected to direct sequencing and classified after phylogenetic analysis. The prevalence of B19 immunoglobulin was 11.1% for IgG and 1% for IgM. B19 DNA was detected in 13.1% of cases. The prevalence of B19 IgG, IgM, and DNA in control group was 25%, 1.6%, and 9.4%, respectively. B19 IgG was significantly lower in HIV group than in normal controls. There was no significant difference regarding anemia between cases and controls. All sequenced B19 isolates belonged to genotype 1A with low genetic diversity. Our findings indicated that in the HAART era, the importance of B19 infections in HIV patients may be limited whereas persistent B19 viremia in the circulation of healthy controls raises a potential concern in blood donations.

Frequency and Genotype of Human Parvovirus B19 among Iranian Hemodialysis and Peritoneal Dialysis Patients

“…Therefore, the prevalence of parvovirus B19 is very variable and the degree of immunodeficiency can explain the differences in the IgG seroprevalence and viremia found in different studies. It seems that dialysis patients have no problem in producing B19 antibodies in accordance with our results, while some studies have reported low rates of B19 IgG among HIV patients due to underlying immunological problems [34,35]. However, persistent B19 infection with coexisting IgG and viremia was found commonly in our HD cases, possibly due to a qualitative defect of antibodies for neutralizing the virus [37,38].…”

“…Previous investigations have described the B19 distribution in different settings and shown a variable epidemiological pattern among populations [32,33,34,35]. However, most surveys mainly focused on the prevalence of B19 in kidney transplant subjects, and few studies have been carried out on the B19 frequency in dialysis patients.…”

“…We previously carried out a study on HIV patients and a control group [35]. The prevalence of B19 IgG, IgM, and DNA in the control group was 25, 1.6, and 9.4%, respectively, while in the HIV group the prevalence of IgG, IgM, and B19 DNA was 11.1, 1, and 13.1%, respectively.…”

Mutations in the Basal Core Promoter and Precore/Core Regions of Hepatitis B Virus in Patients Co-Infected With Human Immunodeficiency Virus

“…Two hundred μl of serum were used for HBV-DNA extraction using commercial high pure viral nucleic acid kits (Roche, Hamburg, Germany), according to manufacturer's instructions. The DNA was amplified by polymerase chain reaction (PCR) using the following pair of primers (GeNet Bio, Korea): (32,33). Similarly, the main modes of transmission in this study were intravenous drug use and history of risky sexual behavior.…”

Detection of Cases of Parvovirus Infection in the System for Epidemiological Surveillance of Exanthematic Diseases

“…За рубежом распространению, диагностике и клиническим проявлениям ПВИ посвящено большое количество исследований [10,11,13,15], однако в РФ работ такого рода явно не достаточ-но [4,8,9].…”