Seroepidemiological Survey of Pathogenic Yersinia in Breeding Squirrel Monkeys in Japan

Iwata, Tadahisa; Une, Yumi; Lee, Ken-ichi; Nakamura, Shinichi; Taniguchi, Takahide; Hayashidani, Hideki

doi:10.1292/jvms.09-0505

Cited by 6 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The serum samples were collected from 6-months-old domestic pigs, stored at −20°C until use, and inactivated at 56°C for 30 min before use. Yops were prepared using a previously described method [ 12 ]. The ELISA was performed according to the method described by Iwata et al [ 12 ].…”

mentioning

confidence: 99%

“…Yops were prepared using a previously described method [ 12 ]. The ELISA was performed according to the method described by Iwata et al [ 12 ]. Briefly, ELISA was performed in flat-bottomed 96-well microtiter plates (MaxiSorp; Nunc, Roskilde, Denmark).…”

mentioning

confidence: 99%

“…Serum obtained from pigs experimentally infected with Y. enterocolitica O8 was used as the positive control. The cutoff value was calculated as the average OD of the negative serum sample plus three standard deviations (SD), as described by Iwata et al [ 12 ]. The Yops antibodies were considered positive when the OD value was higher than the cutoff value.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Seroepidemiological survey of pathogenic <i>Yersinia</i> in domestic pigs in Chiba Prefecture, Japan

NIWA,

IKEUCHI,

BUI

et al. 2024

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

This study aimed to investigate the prevalence of antibodies against pathogenic Yersinia such as Y. enterocolitica and Y. pseudotuberculosis in domestic pigs. A total of 650 serum samples from pigs in nine regions of the Chiba Prefecture in Japan, were tested using plasmid-encoded Yersinia outer membrane protein (Yops) antigen ELISA. The cutoff value was calculated using 20 pathogenic Yersinia -free pig serum samples. According to the cutoff value, 246 (37.8%) pigs from seven regions were considered seropositive for pathogenic Yersinia during the study period. These results indicate that pathogenic Yersinia is widespread in pigs in Chiba, which may become the source of human yersiniosis in this region.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Seroepidemiological survey of pathogenic <i>Yersinia</i> in domestic pigs in Chiba Prefecture, Japan

NIWA,

IKEUCHI,

BUI

et al. 2024

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

Digestive System Diseases of Nonhuman Primates

Brady

Carville²

2012

Nonhuman Primates in Biomedical Research

View full text Add to dashboard Cite

Assessment of the efficacy of an autogenous vaccine against Yersinia pseudotuberculosis in young Merino sheep

Stanger

McGregor

Marenda

et al. 2018

New Zealand Veterinary Journal

View full text Add to dashboard Cite

AIMS To assess the efficacy of an autogenous vaccine against Yersinia pseudotuberculosis III in preventing clinical disease and deaths due to yersiniosis in young Merino sheep, and to determine the effect of vaccination on the prevalence of faecal shedding of pathogenic Yersinia spp., daily liveweight gain, and development of antibodies to Yersinia spp. following vaccination and natural exposure. METHODS In six groups (three groups each from two farms) of young Merino sheep, 148-150 animals were systematically allocated to be vaccinated twice with an autogenous, formalin- killed bacterin vaccine containing Y. pseudotuberculosis serotype III or to remain non-vaccinated. All vaccinated and non-vaccinated sheep were run together in their original groups throughout the trial. Faecal and blood samples were collected, and liveweight measured, at the time of vaccination and subsequently over a 6-month period to determine faecal shedding of Y. enterocolitica and Y. pseudotuberculosis, seroprevalence of antibodies to Yersinia outer membrane proteins (YOP) and changes in liveweight. RESULTS None of the six trial groups experienced an outbreak of clinical yersiniosis during the study period. On Farm A, the prevalence of shedding of either or both Yersinia spp. was <40% on all but one sampling occasions. On Farm B the prevalence of shedding of both Yersinia spp. peaked at 98%, 96 days after vaccination. Mean liveweight and daily liveweight gain at the end of the study were similar in vaccinated and non-vaccinated groups on both farms (p>0.1), as was the prevalence of faecal shedding of Yersinia spp. (p>0.2), and the proportion of animals that became seropositive for antibodies to YOP following vaccination (p>0.1). CONCLUSIONS AND CLINICAL RELEVANCE This vaccine had, at most, limited effects on seroconversion and, under the conditions of this study, had no demonstrable impact on liveweight, mean daily liveweight gain or faecal shedding of Yersinia spp. Further studies are needed to determine the efficacy of this vaccine during outbreaks of yersiniosis or following experimental challenge with pathogenic Yersinia spp..

show abstract

Seroepidemiological Survey of Pathogenic Yersinia in Breeding Squirrel Monkeys in Japan

Cited by 6 publications

References 28 publications

Seroepidemiological survey of pathogenic <i>Yersinia</i> in domestic pigs in Chiba Prefecture, Japan

Seroepidemiological survey of pathogenic <i>Yersinia</i> in domestic pigs in Chiba Prefecture, Japan

Digestive System Diseases of Nonhuman Primates

Assessment of the efficacy of an autogenous vaccine against Yersinia pseudotuberculosis in young Merino sheep

Contact Info

Product

Resources

About