Serofendic Acid, a Novel Substance Extracted From Fetal Calf Serum, Protects Against Oxidative Stress in Neonatal Rat Cardiac Myocytes

Takeda, Toshihiro; Akao, Masaharu; Matsumoto-Ida, Madoka; Kato, Masaya; Takenaka, Hiroyuki; Kihara, Yasuki; Kume, Toshiaki; Akaike, Akinori; Kita, Toru

doi:10.1016/j.jacc.2005.12.055

Cited by 26 publications

(27 citation statements)

References 32 publications

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“…It is a lowmolecularweight (MW 382) compound and exhibits a potent protective effect against neurotoxicity induced by glutamate, NO, and oxidative stress without inhibiting glutamate receptors in cultured cortical, striatal, and spinal cord neurons (23 -26). Moreover, in cardiac myocytes, serofendic acid was found to suppress cell death induced by oxidative stress (27,28). The mechanism of protection remains unclear, but we have demonstrated that serofendic acid preserves mitochondrial functional integrity and inhibits the generation of hydroxyl radical and caspase3 activation (23,24,26,27).…”

Section: Serofendic Acid Protects Against Myocardial Ischemia-reperfumentioning

confidence: 83%

“…It may partially contribute to myocardial protection because ROS is an important inducer of collapsing mitochondrial integrity followed by cell death. In fact, serofendic acid prevented ROS accumulation induced by H2O2 in neonatal rat cardiac myocytes (27). Moreover, serofendic acid inhibited caspase3 activation and reduced TUNELpositive cells in cardiac myocytes and cortical neurons (26,27).…”

Section: Discussionmentioning

confidence: 94%

“…In fact, serofendic acid prevented ROS accumulation induced by H2O2 in neonatal rat cardiac myocytes (27). Moreover, serofendic acid inhibited caspase3 activation and reduced TUNELpositive cells in cardiac myocytes and cortical neurons (26,27). Thus, serofendic acid may exert myocardial protection through the combina tion of these effects.…”

Section: Discussionmentioning

confidence: 95%

“…We previously reported that serofendic acid protected cardiac myocytes against oxidative stress by preventing DYm loss, calcium overload, and ROS accumulation (27,28). The cardioprotective effect can be mediated by mito KATPchannel openers, and mitoKATPchannel blockers prevent both preconditioning and pharmacological cardio protection (34,35).…”

Section: Discussionmentioning

confidence: 99%

“…We previously suggested the involvement of activation of mitoKATP channels in the protective effect of serofendic acid against H2O2 injury in neonatal rat cardiomyocytes (27). Therefore, we examined whether 5hydroxydecanoate (5HD), a mitoKATPchannel blocker, affects the protective effect of serofendic acid in the in vivo model (Fig.…”

Section: Involvement Of Mitochondrial Atp-sensitive Potassium Channelmentioning

confidence: 99%

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Serofendic Acid Protects Against Myocardial Ischemia–Reperfusion Injury in Rats

et al. 2014

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Abstract. We previously reported that serofendic acid, a lipophilic extract of fetal calf serum, protects against oxidative stress in primary culture of neonatal rat cardiomyocytes. However, the effect of serofendic acid on myocardial ischemia-reperfusion injury in vivo is yet to be determined. In the present study, we investigated the effect of intravenous administration of serofendic acid on ischemia-reperfusion injury induced by transient occlusion of the left coronary artery in rats. The rat heart was subjected to 25min ischemia followed by 2h reperfusion. Bolus intravenous administration of serofendic acid (1 -10 mg/kg) given twice reduced the infarct volume in a dose dependent manner. The protective effect of serofendic acid was abolished by pretreatment with 5hydroxydecanoate, a blocker of mitochondrial ATPsensitive potassium channels. For further testing of the protective effect of serofendic acid at the subcellular level, we monitored mitochon drial membrane potential (MMP) in individual cells using realtime twophoton imaging of Langendorffperfused rat heart. A 25min noflow ischemia, followed by reperfusion caused progressive MMP loss. Serofendic acid significantly reduced the number of cells undergoing MMP loss. These results suggest that serofendic acid protected cardiac myocytes against myocardial ischemia-reperfusion injury by preserving the functional integrity of mitochondria.

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Section: Serofendic Acid Protects Against Myocardial Ischemia-reperfumentioning

confidence: 83%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Involvement Of Mitochondrial Atp-sensitive Potassium Channelmentioning

confidence: 99%

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Serofendic Acid Protects Against Myocardial Ischemia–Reperfusion Injury in Rats

et al. 2014

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Corneal Endothelial Cell Damage by Free Radicals Associated With Ultrasound Oscillation

Murano

Ishizaki²,

Satō³

et al. 2008

Arch Ophthalmol

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To determine whether ultrasound oscillations in the anterior chamber cause corneal endothelial injury by free radicals.Methods: A phacoemulsification probe was introduced into the anterior chamber of rabbits' eyes through a limbal incision, and ultrasound oscillation was performed without emulsifying the lens. Rabbits were assigned to 4 treatment groups: (1) no treatment (controls); (2) only irrigation with a salt solution; (3) ultrasound only; and (4) ultrasound oscillations with a salt solution of 0.001M ascorbic acid. The corneas were immunohistochemically examined for oxidative stress using 8-hydroxy-2-deoxyguanosine (8-OHdG), apoptosis by terminal deoxynucleotidyl transferase-mediated dUTPbiotin nick-end labeling (TUNEL) staining, and ultrastructural changes by electron microscopy. The lipid peroxide levels in the aqueous humor were also measured.Results: In the ultrasound-only group, 8-OHdGpositive cells and TUNEL-positive cells were detected at 24 hours; necrotic cells were detected at 12 to 24 hours. Also, lipid peroxide levels were significantly increased at later times in the ultrasound group. Such changes were not observed in other groups. Conclusion:Free radicals induced by ultrasound oscillation can cause corneal endothelial damages.Clinical Relevance: Clinicians should be aware that free radicals associated with ultrasound oscillation can injure the corneal endothelial cells.

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Serofendic acid protects from iodinated contrast medium and high glucose probably against superoxide production in LLC-PK1 cells

et al. 2009

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Serofendic Acid, a Novel Substance Extracted From Fetal Calf Serum, Protects Against Oxidative Stress in Neonatal Rat Cardiac Myocytes

Abstract: Serofendic acid protects cardiac myocytes against oxidant-induced cell death by preserving the functional integrity of mitochondria.

Cited by 26 publications

References 32 publications

Serofendic Acid Protects Against Myocardial Ischemia–Reperfusion Injury in Rats

Serofendic Acid Protects Against Myocardial Ischemia–Reperfusion Injury in Rats

Corneal Endothelial Cell Damage by Free Radicals Associated With Ultrasound Oscillation

Serofendic acid protects from iodinated contrast medium and high glucose probably against superoxide production in LLC-PK1 cells

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