Serologic, Molecular, and Pathologic Survey of Toxoplasma Gondii Infection in Free-Ranging Red Foxes (Vulpes Vulpes) in Central Italy

Verin, Ranieri; Mugnaini, Linda; Nardoni, Simona; Papini, Roberto Amerigo; Ariti, Gaetano; Poli, Alessandro; Mancianti, Francesca

doi:10.7589/2011-07-204

Cited by 26 publications

(14 citation statements)

References 27 publications

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“…Genotyping has previously been carried out in Italy on T gondii DNA from both domestic and sylvatic animals, indicating the occurrence of strictly clonal genotypes in goats 35 and cats, and mixed types detected in free-ranging waterfowl 36 and foxes. 37 These results are in agreement with the hypothesis of two distinct cycles of T gondii in domestic and sylvatic environments. 38 Eleven cats were positive for G duodenalis DNA, with an overall prevalence of 7.5%, which is in agreement with Paoletti et al 18 Genotyping yielded nine assemblages, with F the most frequent in cats and C the most common in dogs.…”

supporting

confidence: 89%

A retrospective molecular study of select intestinal protozoa in healthy pet cats from Italy

Mancianti

Nardoni

Mugnaini

et al. 2014

Journal of Feline Medicine and Surgery

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The feline gut can harbour a number of protozoan parasites. Recent genetic studies have highlighted new epidemiological findings about species of Cryptosporidium, assemblages of Giardia duodenalis and Toxoplasma gondii. Furthermore, epidemiological studies suggest the occurrence of Tritrichomonas foetus in cats is on the increase worldwide. The prevalence of selected intestinal protozoa was determined by PCR using DNA previously extracted from the faeces of 146 privately owned healthy cats from Italy. Molecular genotyping on T gondii, G duodenalis and Cryptosporidium DNA was achieved. PCR assays were positive in 32 (22.9%) samples. Three animals (2.0%) were positive for T foetus and Cryptosporidium DNA, 15 specimens (10.3%) were positive for T gondii and 11 (7.5%) for G duodenalis. Co-infections were never observed. Results of the typing analysis allowed the identification of Cryptosporidium felis in all cases. The specimens positive for T gondii hinted at clonal genotype I (n = 7), genotype II (n = 1) and genotype III (n = 7). The G duodenalis isolates were referable to assemblages F (n = 9) and C (n = 2). In conclusion, the results obtained in this study add to the literature regarding the epidemiology of these parasites by confirming their presence in the faeces of healthy pet cats.

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supporting

confidence: 89%

A retrospective molecular study of select intestinal protozoa in healthy pet cats from Italy

Mancianti

Nardoni

Mugnaini

et al. 2014

Journal of Feline Medicine and Surgery

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“…In Spain, antibodies to N. caninum were demonstrated by cELISA and confirmed by IFAT in 3.2% of 95 red foxes [6], similar to the presented study; also similar to this study, a high T. gondii seroprevalence (100%) was found in 123 foxes in Belgium [7]. Antibodies to T. gondii were detected by immunoblot analysis in 74.5% of 204 and 84.7% of 176 red foxes in Germany [8], and by IFAT in 53.4% of 191 foxes in Italy [9]. Higher T. gondii prevalence compared to N. caninum was detected also in other countries.…”

Section: Discussionsupporting

confidence: 91%

Neospora caninum and Toxoplasma gondii antibodies in red foxes (Vulpes vulpes) in the Czech Republic

Bártová

Slezáková

Nagl

et al. 2015

Ann Agric Environ Med.

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Introduction and objective. Neospora caninum and Toxoplasma gondii are worldwide spread parasites, causing serious illnesses in sensitive animals; toxoplasmosis is also important zoonosis. Although neosporosis is not considered as a zoonosis, it leads to aborted births in cattle, as well as paresis and paralysis in dogs. Objective. The aim of this study was to discover the prevalence of N. caninum and T. gondii antibodies in red foxes (Vulpes vulpes) in the Czech Republic. Materials and method. Sera of 80 foxes from 8 regions of the Czech Republic were tested for antibodies to N. caninum and T. gondii by competitive enzyme linked immunosorbent assay (cELISA) and indirect ELISA. All samples were simultaneously tested by indirect fluorescent antibody test (IFAT) to detect both N. caninum and T. gondii antibodies. Results. Antibodies to N. caninum were found by IFAT in 3 (3.8%) red foxes with titre 50 and in 2 (2.5%) red foxes with inhibition 42.7% and 30.2 %. Antibodies to T. gondii were found in all tested animals in both IFAT (titres 50-6400) and in ELISA (S/P ranging from 34%-133%). Conclusion. This is the first prevalence study of N. caninum and T. gondii antibodies in red foxes in the Czech Republic. The results obtained show that red foxes are exposed at different levels to both protozoan infections, and thus could play an important role in the transmission cycle of N. caninum and T. gondii in sylvatic cycle.

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“…This is the first report of a T. gondii genotype in wild Italian ungulates. Despite having used different protocols, Verin et al (2013) demonstrated the occurrence of polymorphic strains (combination of different type I and III alleles) in red foxes (Vulpes vulpes) and Mancianti et al (2013Mancianti et al ( , 2014 detected genotypes I and III and atypical genotypes (with hints of genotype I) in goats and genotypes II and III in donkeys. Therefore, all the T. gondii genotypes appear to be present in Italy.…”

Section: Discussionmentioning

confidence: 99%

Spread and genotype of Toxoplasma gondii in naturally infected alpine chamois (Rupicapra r. rupicapra)

et al. 2016

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Serologic, Molecular, and Pathologic Survey of Toxoplasma Gondii Infection in Free-Ranging Red Foxes (Vulpes Vulpes) in Central Italy

Cited by 26 publications

References 27 publications

A retrospective molecular study of select intestinal protozoa in healthy pet cats from Italy

A retrospective molecular study of select intestinal protozoa in healthy pet cats from Italy

<i>Neospora caninum</i> and <i>Toxoplasma gondii</i> antibodies in red foxes (<i>Vulpes vulpes</i>) in the Czech Republic

Spread and genotype of Toxoplasma gondii in naturally infected alpine chamois (Rupicapra r. rupicapra)

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