Serologic recognitionof hydatid cyst antigens using different purification methods

Sbihi, Younes; Janssen, Dirk; Osuna, Antonio

doi:10.1016/0732-8893(96)00061-2

Cited by 34 publications

(32 citation statements)

References 13 publications

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“…We recently showed that certain methods to purify parasite antigens can strongly improve the sensitivity and specificity of these assays (5). Nevertheless, although there is a general agreement on the need for antigens that enable specific and sensitive diagnosis of hydatidosis, the specific antibodies that are screened for are mostly of the IgG class.…”

Section: Introductionmentioning

confidence: 98%

Specific recognition of hydatid cyst antigens by serum IgG, IgA using Western blot

Sbihi

Janssen

Osuna

1997

J. Clin. Lab. Anal.

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Section: Introductionmentioning

confidence: 98%

Specific recognition of hydatid cyst antigens by serum IgG, IgA using Western blot

Sbihi

Janssen

Osuna

1997

J. Clin. Lab. Anal.

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“…Alternatively, purified fractions of antigen 5 or B have been similarly tested (1,9,10,27,34,36), However, all of these antigens still exhibit some problems, mainly related to temporally delayed reactions to clinical changes (2). Thus, the persistence of specific antibodies against cyst fluid antigens for several years after recovery makes it difficult to predict the outcome of surgery early enough (5,21,29).…”

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confidence: 99%

Assessment of Echinococcus granulosus Somatic Protoscolex Antigens for Serological Follow-Up of Young Patients Surgically Treated for Cystic Echinococcosis

et al. 2008

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Echinococcus granulosus protoscolex soluble somatic antigens (PSSAs) were assessed for their prognostic value in the serological follow-up of young patients treated for cystic echinococcosis (CE), compared to conventional hydatid fluid (HF) antigen. Based on different clinical courses and outcome of infection, as well as imaging findings, patients were retrospectively classified into two different groups including either cured CE (CCE; i.e., absence of active cysts or presence of inactive cysts, respectively) and noncured CE (NCCE) patients still presenting active cysts at the end of an up to 5-year follow-up period. An immunoglobulin G (IgG)-PSSA enzyme-linked immunosorbent assay (ELISA) showed a gradual decrease in antibody levels in CCE cases, reaching seronegativity in 20% of the cases at least within 5 years postsurgery. In comparison, the conventional IgG-HF ELISA showed a significantly lower progressive decrease in antibody levels, serology becoming negative in only 15% of CCE patients at the endpoint of the follow-up period. Serological analysis of PSSA by immunoblotting yielded an interesting immunoreactive double band of 27 and 28 kDa that, in 15 (75%) of 20 CCE cases, exhibited a rapid decrease and subsequent disappearance of respective antibody reactivities within 3 years postsurgery. Conversely, anti-27-and -28-kDa antibody reactivity strongly persisted until the endpoint of the follow-up period in all of the five NCCE patients. Further analysis of the 27-and 28-kDa doublet by using affinity-purified antibodies showed that the double band was not detectable in HF. Furthermore, a predominantly IgG4 subclass-restricted humoral immune response against the 27-and 28-kDa antigens was demonstrated in seroreactive CE patients. Overall, an anti-27-and -28-kDa response appeared to correlate with cyst activity. In conclusion, PSSA represents a useful candidate to carry out a serologic follow-up of CE subsequent to treatment and deserves further respective evaluation for other age groups of CE patients.

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“…The parasitic antigens of major diagnostic value are antigen 5 (arc-5) and antigen B (8,16). Purified fractions enriched in antigens 5 and B and glycoproteins from hydatid fluid yielded a sensitivity rate of 95%, with a specificity rate of 100% (17). The diagnosis of hydatid disease was confirmed in all our patients by positive IFA test, CT findings and pathognomonic surgical findings.…”

Section: Discussionmentioning

confidence: 58%

Increased anti-Echinococcus granulosus antibody positivity in Fasciola hepatica infection

Kaya

Beştaş²,

Gırgın³

et al. 2012

Turk J Gastroenterol

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Serologic recognitionof hydatid cyst antigens using different purification methods

Cited by 34 publications

References 13 publications

Specific recognition of hydatid cyst antigens by serum IgG, IgA using Western blot

Specific recognition of hydatid cyst antigens by serum IgG, IgA using Western blot

Assessment of Echinococcus granulosus Somatic Protoscolex Antigens for Serological Follow-Up of Young Patients Surgically Treated for Cystic Echinococcosis

Increased anti-Echinococcus granulosus antibody positivity in Fasciola hepatica infection

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