Serologic Testing for Avian Influenza Viruses in Wild Birds: Comparison of Two Commercial Competition Enzyme-Linked Immunosorbent Assays

Pérez-ramírez, Elisa; Rodríguez, Vanessa; Sommer, D.; Blanco, Juan Manuel; Acevedo, Pelayo; Heffels-Redmann, U.; Höfle, Úrsula

doi:10.1637/9207-880209-digest.1

Cited by 5 publications

(8 citation statements)

References 7 publications

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“…1 Over the last decade, multiple commercial ELISAs have been developed for detection of FLUAV antibodies in wild birds, and it is necessary to evaluate the performance of such tests in order to compare results and conclusions derived from studies utilizing these assays. 1,11 In the current study, 2 commercial ELISAs for rapid screening of FLUAV nucleoprotein (NP) antibodies were tested. All FLUAV subtypes share the NP antigen and present little genetic variation, as compared to the hemagglutinin and neuraminidase proteins; therefore, the NP antigen represents a type A influenza-specific antigen.…”

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confidence: 99%

Comparison of two commercial enzyme-linked immunosorbent assays for detection of Influenza A virus antibodies

et al. 2011

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Serologic tools for Influenza A virus (FLUAV) antibody testing of wild birds are currently limited. In the present study, 2 commercial enzyme-linked immunosorbent assays (ELISAs) for detection of FLUAV antibodies, the IDEXX AI MultiS-Screen Ab Test and the ID VET ID Screen Influenza A Antibody Competition, were compared. Sera obtained from mallards (Anas platyrhynchos), experimentally infected with 8 FLUAV subtypes (N = 48), and field serum samples, collected from 11 wild bird species (N = 247), were tested. Overall, a substantial agreement was obtained between the 2 assays as applied to both experimental (86.5% agreement, κ = 0.69) and field samples (89.9% agreement, κ = 0.78). Based on the current study, doubtful results obtained with the ID VET assay should be re-tested to confirm their antibody status. Additionally, increasing the incubation period for the ID VET assay increases the test sensitivity but also increases the likelihood of generating false-positive results. Overall, it is concluded that the 2 ELISAs can be used for FLUAV antibody screening in wild birds and that the sensitivity of the ID VET assay can be increased with slight modifications of the manufacturer's instructions.

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Comparison of two commercial enzyme-linked immunosorbent assays for detection of Influenza A virus antibodies

et al. 2011

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“…Testing for antibodies to Influenza A (IA) virus is a common diagnostic tool used in poultry and also has recently been incorporated into wild bird surveillance efforts . These assays usually are based on the detection of IA virus nucleoprotein antibodies using agar gel immunodiffusion or enzyme‐linked immunosorbent assays (ELISA), and hemagglutinin (HA) and neuraminidase antibodies using hemagglutination inhibition (HI) and neuraminidase inhibition tests, respectively.…”

Section: Effect Of the Incubation Period And Sample Dilution Factor Omentioning

confidence: 99%

“…These assays usually are based on the detection of IA virus nucleoprotein antibodies using agar gel immunodiffusion or enzyme‐linked immunosorbent assays (ELISA), and hemagglutinin (HA) and neuraminidase antibodies using hemagglutination inhibition (HI) and neuraminidase inhibition tests, respectively. Several commercial ELISA's have recently been developed and evaluated for use in both poultry and wild birds …”

Section: Effect Of the Incubation Period And Sample Dilution Factor Omentioning

confidence: 99%

Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

Lebarbenchon

Pantin‐Jackwood

Kistler

et al. 2012

Influenza Resp Viruses

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The ID Screen Influenza H5 Antibody Competition enzyme‐linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5‐infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.

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“…In regards to other tests used to detect AIV antibodies, a cELISA was more sensitive and specific than the agar gel immuno diffusion (AGID) test [4,9], and as or more sensitive and specific as the haemagglutination inhibition (HI) test [3,4,9,12]. A cELISA was also able to detect antibodies at an earlier stage of infection compared with the AGID and HI tests [5,7,11,13]. Therefore, cELISAs are useful multi species diagnostic tests.…”

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Adaptations of a Competitive Enzyme-Linked Immunosorbent Assays for the Detection of Antibodies to Influenza A Virus in Horse Sera for Use in Wild Aquatic Birds

et al. 2012

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We applied a competitive enzyme-linked immunosorbent assay for the detection of antibodies for influenza A in equine sera to their detection in sera from wild aquatic birds. Suboptimal results were obtained for the optical density (OD) of the monoclonal antibody (MAb) control and reproducibility between duplicate analyses in the initial assessment. It was therefore necessary to modify the assay to deliver increased reliability and reproducibility while maintaining adequate sensitivity. We optimized reagent concentrations to obtain optimal OD values (close to 2) for the monoclonal antibody control and used 2, 2'-Azino-bis: 3-Benzthiazoline-6-Sulphonic Acid as an alternative chromogen to potentially reduce variability in duplicate analyses. The original assay was compared with the optimized versions, with and without post coating, for the detection of avian influenza viral antibodies in 240 sera obtained from wild plumed whistling ducks. A separate analytical sensitivity study on diluted positive field sera of plumed whistling ducks and a test of antigen stability after post coating were also performed. Some quantitative differences were detected between the original and modified assays. The original assay recorded higher percentage inhibition results which were potentially indicative of increased sensitivity. However, when reagent concentrations were increased in the original assay to the same levels as used in the modified versions, there were no quantitative differences for practical purposes. The original assay produced a median (OD) value of 0.81 for the (MAb) controls that is at the limit of acceptability. By contrast, the modified assays always produced acceptable optical density values for MAb controls. Our overall results indicated the modified assays were potentially more reliable (OD values close to 2), and of adequate sensitivity compared to the original assay in the detection of avian influenza viral antibodies in wild bird sera. Although further optimization of antigen and MAb concentrations should also be considered to increase the sensitivity of a modified assay, while maintaining acceptable optical density values for the MAb control. Post coating had a minimal quantitative effect on the results and stabilized the plates for 214 days. We therefore recommend the incorporation of post coating.

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Serologic Testing for Avian Influenza Viruses in Wild Birds: Comparison of Two Commercial Competition Enzyme-Linked Immunosorbent Assays

Cited by 5 publications

References 7 publications

Comparison of two commercial enzyme-linked immunosorbent assays for detection of Influenza A virus antibodies

Comparison of two commercial enzyme-linked immunosorbent assays for detection of Influenza A virus antibodies

Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

Adaptations of a Competitive Enzyme-Linked Immunosorbent Assays for the Detection of Antibodies to Influenza A Virus in Horse Sera for Use in Wild Aquatic Birds

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