Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam

Abstract

Avian Metapneumovirus (aMPV) is a causative agent of respiratory disease complex in turkeys and chickens that has recently been detected in Vietnam. Due to its novelty, this study was conducted to elucidate the distribution of aMPV in several provinces in northern Vietnam. By the application of Enzyme-Linked Immunosorbent Assay (ELISA) and nested Reverse Transcription-Polymerase Chain Reaction (RT-PCR), this study demonstrated the circulation of aMPV in 12 out of 14 cities/provinces with positive rates of 37.6% and 17.2%, respectively. All nested RT-PCR positive samples were aMPV subgroup B. By pairing the detection results with age groups, it was observed that aMPV infections occurred in chickens of all ages. Additionally, by genetic characterization, aMPV strains were demonstrated to not be attenuated vaccine viruses and to belong to at least two genetic clades. Overall, the obtained results provided insights into the prevalence of aMPV and indicated a greater complexity of respiratory diseases in chickens in Vietnam.

Complete Genome Sequences of Avian Metapneumovirus Subtype B Vaccine Strains from Brazil

“…Strains BR/1890/E1/19 and BR/1891/E2/19 are most similar to VCO3/60616 (identities, 99.79% and 99.48%, respectively) ( 17 – 19 ) ( Fig. 1A ), but the G-gene sequences phylogenetically group BR/1890/E1/19 with Vietnamese Nemovac and Hipraviar vaccine strains ( 20 ) in a separate cluster from BR/1891/E2/19 ( Fig. 1B ).…”

Establishment and Application Prospect of Reverse Transcriptase Recombinase-Aided Amplification Assay for Subgroup C Avian Metapneumovirus

“…In 1978, aMPV was found in South Africa and has since been widespread worldwide. As of now, aMPV has been found in many areas where poultry is raised in Europe, Asia, South America, Africa, and North America [ 8 , 9 , 10 , 11 , 12 , 13 ]. It can be seen that aMPV has an extensive spread range.…”