Serological, Biochemical, and Epidemiological Studies on Sarcocystis in Sheep and Goats in Assuit Governorate. Egypt

Osman, Fathy Ahmad; Gaadee, Hoda Ibrahiem; M., Sara Abdel-Aal

doi:10.2139/ssrn.4204394

Cited by 3 publications

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“…Moreover, our infection rate with S. gigantea in sheep (0.01%) was also remarkably lower than in sheep slaughtered in Iran in 2011 [29.3%; ( 28 )], Romania in 2007 [26.3%, ( 7 )], and Egypt in 2022 [42.7%; ( 15 )].…”

Section: Discussionmentioning

confidence: 53%

“…Our results are confirmed by a previous study in Sulaimany province, where no macroscopic sarcocystosis was found in sheep (0.0%) in a relatively small sample only 130 tissue samples ( 28 ). In contrast, the prevelance of macroscopic sarcocystosis was remarkably higher in goats (33.6%) infected, during the winter season 1991–1992 ( 11 ), and in goats in Egypt 35.5% ( 15 ) with S. caprafelis moulei and originating from the same region: a slaughterhouse in Sulaimany province.…”

Section: Discussionmentioning

confidence: 94%

“…The prevalency of macroscopic sarcocystosis in domestic sheep varied regionally: 4.1% in Iraq ( 13 ), 29.3% in Iran ( 14 ), and 42.7% in Egypt ( 15 ). In domestic goats macrocysts occurred 33.6% in Kurdistan/Iraq ( 11 ), 16.6% in Iran ( 16 ) and 35.5% in Egypt ( 15 ). There is but a single, brief description of the S. moulei macrocysts ultrastructure in Egyptian goats ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

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Morphological, ultrastructural, genetic characteristics and remarkably low prevalence of macroscopic Sarcocystis species isolated from sheep and goats in Kurdistan region, Iraq

Nawshirwan,

Heucken,

Piekarek

et al. 2023

Front. Vet. Sci.

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IntroductionSarcocystis is a genus of cyst-forming parasites that infest both humans and livestock. Some parasites cause clinical and subclinical diseases in their hosts, resulting in economic losses.MethodsEsophagus, diaphragm, and skeletal muscle from slaughtered sheep and goats were examined macroscopically, microscopically, and ultrastructurally and subjected to DNA analysis.ResultsWe isolated macrocysts of S. gigantea and of S. caprafelis moulei from naturally infected sheep (Ovis aries) and goats (Capra hircus). The macrocyst wall thickness was 18.9 µm in sheep and 15.3 µm in goats, and consisted of an inner Periodic acid Schiff- (PAS) negative primary wall and an outer glycoconjugates containing i.e. PAS-positive secondary wall. The walls inner surface was compartmentalized and filled with bradyzoites. In S. gigantea the bradyzoites were approximently 12.3 x 2.6 µm in size, while in S. caprafelis moulei they were 13.9 x 4.4 µm. Ultrastructurally, both species have nearly identical morphology: cauliflower-like protrusions with numerous microtubules and often dendritic-like filaments, branching from the primary wall. The 18S rRNA gene in S. gigantea was 85.9% identical to that in S. medusiformis and 80.4% to the S. caprafelis moulei gene. The 28S rRNA gene in S. gigantea was 94.6% identical to that in S. medusiformis and 97.3% to the S. caprafelis moulei.ConclusionThis study is the first to (i) detail the ultrastructure of the macrocyst wall of S. caprafelis moulei, (ii) identify S. medusiformis in Iraqi sheep, and (iii) compare the prevalence of macroscopic Sarcocystis at different time periods within the same region. A positive finding was the reduction of macroscopic sarcocystosis occurrences (0.01% in sheep and 0.02% in goats) compared to our previous data from 1992 (4.1%: sheep, 33.6%: goats).

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Morphological, ultrastructural, genetic characteristics and remarkably low prevalence of macroscopic Sarcocystis species isolated from sheep and goats in Kurdistan region, Iraq

Nawshirwan,

Heucken,

Piekarek

et al. 2023

Front. Vet. Sci.

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“…According to the study, the lifespan and life cycle of the spider. Sallam (1996), El-Erksousy et al, (2002), El-Hennawy and Mohafez (2003), Ahmed (2012) and Rashwan (2017).…”

Section: Efficiency Of Scytodes Univittata Spider On Various Prey Con...mentioning

confidence: 99%

Studies on Scytodes Univittata Simon (Scytodidae: Arachnida) with Special Notes on its Biology

Ahmed,

Rashwan

2024

Journal of Plant Protection and Pathology

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This study's aims to examine the age of Scytodes univittata Simon, 1882, (Scytodidae: Arachnida). This species was discovered between and under stones on the ground in the mango groves in Tahta, Sohag governorate, Egypt, Males had an average life cycle of 392.8±7.39 days, while females completed its life cycle in 484.0 ±3.20 days. The first and second stages of the investigated spider consumed the greatest number of the prey species Aphis spp., and Larvae in their first three instars Spodopetra littorali (Boisd.), In contrast, the third and fourth spiderling instars, which dined on the first through third instars of S. littoralis and larvae of Galleria mellonella (Linn.). Spider individuals fed on various phases of larvae and adults of spiderlings from fifth instars to adulthood of S. littoralis and larvae of G. mellonella. The female spiders lived for an average of 309.8± 8.01 days.

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“…Haemonchosis is regarded as a highly destructive disease that significantly reduces the productivity of infected sheep and has substantial economic consequences. This includes elevated levels of morbidity and mortality and increased management costs associated with implementing control measures ( 6 , 7 ). The disease is caused by Haemonchus spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of Haemonchus contortus in sheep from Upper Egypt

Mohamed,

Dyab,

Raya-Álvarez

et al. 2024

Front. Vet. Sci.

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IntroductionHaemonchus spp. are considered the most important strongylid nematodes with a worldwide distribution. The parasite’s blood-sucking nature can lead to severe anemia in infected animals. Despite its widespread impact, there is a dearth of comprehensive data on morphological and molecular identification methods for Haemonchus spp. in sheep from Upper Egypt. To address this gap, our current study aimed to assess the prevalence of Haemonchus spp. in 400 sheep fecal samples.MethodsWe employed microscopic examination and molecular techniques, using polymerase chain reaction (PCR) targeting the 18S gene for precise identification. Additionally, the potential risk factors associated with the infection by the parasite in sheep were explored.ResultsThe study pointed out that 33.00% (132 of 400) of the examined sheep were infected with Haemonchus spp. Sheep age and seasonal variability were found to be significant factors (p < 0.05) associated with the infection. Notably, sheep under 2 years old exhibited a higher risk, with an infection rate of 43.75% (84 out of 192), than their older counterparts. Furthermore, all reported infections were exclusively observed during the cold season, constituting 58.93% (132 out of 224) of cases. By contrast, no statistically significant association (p > 0.05) was found between the sex of the examined sheep and the occurrence of haemonchosis. Employing molecular methods, we isolated and identified the parasite through PCR analysis of cultured larvae, which were then subsequently confirmed as Haemonchus contortus via phylogenetic analysis.DiscussionThe study concluded that there was a relatively high occurrence of H. contortus among sheep from Upper Egypt. We recommend the implementation of stringent and effective control measures to combat the infection and safeguard livestock health.

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Serological, Biochemical, and Epidemiological Studies on Sarcocystis in Sheep and Goats in Assuit Governorate. Egypt

Cited by 3 publications

References 0 publications

Morphological, ultrastructural, genetic characteristics and remarkably low prevalence of macroscopic Sarcocystis species isolated from sheep and goats in Kurdistan region, Iraq

Morphological, ultrastructural, genetic characteristics and remarkably low prevalence of macroscopic Sarcocystis species isolated from sheep and goats in Kurdistan region, Iraq

Studies on Scytodes Univittata Simon (Scytodidae: Arachnida) with Special Notes on its Biology

Molecular identification of Haemonchus contortus in sheep from Upper Egypt

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