Serological Characterization of Human Monocytes for HLA, B‐Lymphocyte, Granulocyte, and Monocyte‐Associated Antigens by Cytotoxicity Testing

Drew, SI; Nathanson, David; Zamfirescu, P.; Carter, B. M.; Terasaki, Paul I.; Naiem, Faramarz; Billing, Ronald

doi:10.1111/j.1399-0039.1978.tb01272.x

Cited by 13 publications

(5 citation statements)

References 13 publications

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“…Thirdly, TB21 was preferentially detected on B cells, but not on monocytes, by the complement-dependent cytotoxicity test. DR and MT antigens were detected on monocytes as well as B cells, as previously reported (Albrechsten 1977, Stastny 1978, Drew et al 1978). Such a difference in cellular expression again supports the possibility that TB21 is a gene product of a locus distinct from DR and MT.…”

Section: Discussionsupporting

confidence: 85%

A new B‐cell alloantigen, TB21, coded for in the HLA‐D/DR region

Maeda¹,

Juji

1982

Tissue Antigens

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A new B-cell alloantigen, designated as TB21, was serologically defined by 2 monospecific sera, T383 and T2200. Both T383 and T2200 had cytotoxic titers of 1:8 against TB21 positive B cells and were highly correlated to each other (r = 0.876). The phenotype frequency of TB21 was approximately 5 5 % in the Japanese population. TB21 was strongly associated with DRw9 and weakly with DR5, hut was definitely distinct from the known supertypic specificities such as MT or MB antigens. Family studies showed that TB21 segregated with different DR antigens on different haplotypes, indicating that TB21 is coded for by a gene at a closely linked but distinct locus from HLA-DR. Furthermore, TB21 was preferentially detected on B cells but not on monocytes by complement-dependent cytotoxicity. Failure of detection of TB21 on monocytes further distinguished the TB21 antigen from DR and MT antigens. Results of this study suggest that there exist 2 kinds of B-cell alloantigens coded in the HLA region: DR and MT antigens, and other B-cell alloantigens represented by TB21.

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Section: Discussionsupporting

confidence: 85%

A new B‐cell alloantigen, TB21, coded for in the HLA‐D/DR region

Maeda¹,

Juji

1982

Tissue Antigens

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“…In general, antigens detected by granulocytotoxins appear to be restricted to cells of the myeloid lineage, namely mature cord and adult neutrophils, eosinophils, certain CML blast cell populations and a CML cell line in culture (K562). Interestingly, monocytes which are believed to originate from the same stem cell precursor as granulocytes (Leder 1967) lack granulocyte antigens (Drew, et al, 1978b). In addition, T and B lymphocytes and platelets did not appear to possess antigens recognized by the cytotoxic granulocyte antisera.…”

Section: Granulocyte Antigens and Granulocytotoxinsmentioning

confidence: 99%

Cell Surface Antigens Detected on Mature and Leukemic Granulocytic Populations by Cytotoxicity Testing

et al. 1978

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Using a microcytotoxicity assay, the serological reactivity of human granulocytes, namely neutrophils and eosinophils, and chronic myeloid leukemia (CML) cells and cultured CML cell lines (K562, NALM-1) were examined. Mature granulocyte forms and cord granulocytes are readily lysed by specific granulocyte cytotoxins that do not react with random T and B lymphocytes, monocytes, red blood cells, or platelets. Furthermore, certain antisera were preferentially cytotoxic for eosinophil-enriched populations. Granulocytotoxin detected antigens on one of three CML blast cell populations tested and K562, but failed to react with NALM-1. By cytotoxicity, mature granulocytes were poor targets for B2-microglobulin and the appropriate HLA antisera although both sera types are absorbed with granulocytes. Furthermore, granulocytes did not possess B-lymphocytes (Ia-like) or blood group A, B, and Rh (D) antigens. Except for K562, both HLA and heterologous B-lymphocyte antisera were cytotoxic for the CML blast cell populations tested.

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“…Extra reactions somewhat complicated interpretation of the monocyte typing data. They have been reported by Drew et al (1978) and by Cicciarelli et al (1978) and may be due to the presence in antisera of antibodies directed against monocyte HLA-DR products or against monocyte-unique specificities. Theoretically, anti-HLA-DR antibodies could have been eliminated by adsorbing antiserum on pooled B-cells.…”

Section: Discussionmentioning

confidence: 71%

HLA‐A, ‐B, and ‐DR specificities on human monocytes

Merdrignac¹,

Fauchet²,

Bouhallier³

et al. 1981

Tissue Antigens

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Monocyte-enriched cell suspensions obtained by a plate adherence method from 57 blood donors were typed by microcytotoxicity for HLA-A, -B and -DR determinants. Parallel assays were performed with autologous unfractionated lymphocytes for HLA-A and -B determinants and with autologous B-lymphocytes for HLA-DR determinants. Correlation coefficients (r values) were calculated for nine HLA-A specificities (r greater than 0.79), 16 HLA-B specificities (r greater than 0.56) and seven HLA-DR specificities (r greater than 0.81). For certain HLA-A and -B specificities detection was less readily achieved on monocytes than on autologous lymphocytes. Extra reactions were observed in some instances. With sera defining HLA-DR specificities, monocytes and B-cells showed reactivity of almost identical strength and reliability.

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Serological Characterization of Human Monocytes for HLA, B‐Lymphocyte, Granulocyte, and Monocyte‐Associated Antigens by Cytotoxicity Testing

Cited by 13 publications

References 13 publications

A new B‐cell alloantigen, TB21, coded for in the HLA‐D/DR region

A new B‐cell alloantigen, TB21, coded for in the HLA‐D/DR region

Cell Surface Antigens Detected on Mature and Leukemic Granulocytic Populations by Cytotoxicity Testing

HLA‐A, ‐B, and ‐DR specificities on human monocytes

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