Serological Characterization of <i>Trichosporon cutaneum</i> and Related Species

The phylogenetic and taxonomic heterogeneity of a rare opportunistic yeast pathogen, Cryptococcus humicolus, was revealed by analysis of the sequence of the internal transcribed spacer (ITS) region. Sixteen strains of C. humicolus showed a wide diversity in their ITS sequences. In addition, their 18S rDNA sequences were determined and used to analyze the phylogenetic relationships among C. humicolus and related yeasts. On trees constructed by the Neighbor-Joining and Maximum Parsimony methods, C. humicolus strains were phylogenetically closely related to each other with the exception of one strain, and they clustered with C. curvatus and Trichosporon species with high bootstrap values. Three C. humicolus strains obtained from humans belonged to the group of Trichosporon serotype I species. The results suggest that C. humicolus is a genetically heterogeneous species which should be reclassified on the basis of DNA sequence data.

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“…Strains CBS 1896, 1987 and 2043 were serotyped with specific factor sera for Trichosporon as described by Ikeda et al (6).…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic and Taxonomic Heterogeneity of Cryptococcus humicolus by Analysis of the Sequences of the Internal Transcribed Spacer Regions and 18S rDNA, and the Phylogenetic Relationships of C. humicolus, C. curvatus, and the Genus Trichosporon

Sugita

Takashima

Ikeda

et al. 2000

Microbiology and Immunology

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“…Since 1984, when Shimazu et al (15) first reported that SHP was induced by Trichosporon, the number of patients reported to suffer from SHP has been increasing steadily. When the role of Trichosporon in SHP was first ascertained, T. cutaneum was considered to be the causative antigen, and four serotypes were identified, namely I, II, III, and I-III (9,12). Patients with SHP had positive reactions to inhalation challenge with antigens of the same serotype of T. cutaneum isolated from their houses, indicating that patients are sensitized by exposure to the dominant Trichosporon species in their home environment (3).…”

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confidence: 99%

Analysis of Trichosporon Isolates Obtained from the Houses of Patients with Summer-Type Hypersensitivity Pneumonitis

Sugita¹,

Ikeda²,

Nishikawa

2004

J Clin Microbiol

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Summer-type hypersensitivity pneumonitis (SHP) is type III or IV allergies developed by repeated inhalation of arthroconidia of Trichosporon species. We identified 105 strains obtained from the homes of 36 SHP patients by analysis of the intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes; in addition, we analyzed the IGS genotypes of the strains. Serologically, Trichosporon species are classified as serotype I, II, III, or I-III. Of the 105 strains, 43 (41.1%), 53 (50.5%), and 9 (8.6%) strains were isolated as serotypes I, II, and III, respectively. Serotype I, II, and III strains were recovered from 19 (52.8%), 29 (80.6%), and 4 (11.1%) of the 36 houses of SHP patients, respectively. No serotype I-III strains were isolated from the houses. Of 43 serotype I strains, 42 (97.7%) were identified as Trichosporon dermatis, and the remaining one was T. terricola. Of 53 serotype II strains, 37 (69.8%) were identified as T. asahii, and the remaining serotype II isolates were T. aquatile (1.9%), T. coremiiforme (7.5%), T. faecale (1.9%), T. japonicum (15.1%), and T. ovoides (3.8%). There were nine serotype III strains comprised of T. montevideense (77.8%) and T. domesticum (22.2%). Intraspecies diversity was found only in T. asahii. This microorganism also causes opportunistic infections (trichosporonosis); seven genotypes of its IGS 1 region have been identified. While the strains of T. asahii obtained from Japanese patients with trichosporonosis were genotype I, the strains from the houses of SHP patients were genotype III. Based on our analysis, we conclude that the strains that play the most significant roles in the development of SHP are T. dermatis, T. asahii genotype 3, and T. montevideense, representing serotypes I, II, and III, respectively.

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“…Serotype was determined by a slide agglutination test using specific-factor sera as described by Ikeda et al (6).…”

Section: Methodsmentioning

confidence: 99%

“…Since 1984, when SHP caused by Trichosporon species was first reported by Shimazu et al (12), the number of patients reported to suffer from this disease has been increasing steadily. Serologically, at least four different types (I, II, III and I-III) have been identified in the Trichosporon species (6,10). Most of the environmental strains isolated from SHP patients' houses are serotype I or II.…”

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confidence: 99%

First Report of Trichosporon ovoides Isolated from the Home of a Summer‐Type Hypersensitivity Pneumonitis Patient

Sugita

Nishikawa

Ikeda

et al. 1998

Microbiology and Immunology

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Trichosporon species have been known to cause summer-type hypersensitivity pneumonitis (SHP). During the isolation of yeasts from an SHP patient's house, we recovered a strain belonging to the genus Trichosporon. Morphologically, the isolate produced rectangular arthroconidia when grown on corn meal agar. DNA-DNA hybridization experiments identified the isolate as T ovoides. A slide agglutination test using specific factor sera demonstrated that the serotype of the strain was type II. Previously, T asahii, a serotype II species, was considered to be the major antigen of SHP, but it is possible that T ovoides may also be responsible for SHP. This is the first report of T ovoides isolated from an SHP patient's home environment.

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Serological Characterization of Trichosporon cutaneum and Related Species

Cited by 21 publications

References 18 publications

Phylogenetic and Taxonomic Heterogeneity of Cryptococcus humicolus by Analysis of the Sequences of the Internal Transcribed Spacer Regions and 18S rDNA, and the Phylogenetic Relationships of C. humicolus, C. curvatus, and the Genus Trichosporon

Phylogenetic and Taxonomic Heterogeneity of Cryptococcus humicolus by Analysis of the Sequences of the Internal Transcribed Spacer Regions and 18S rDNA, and the Phylogenetic Relationships of C. humicolus, C. curvatus, and the Genus Trichosporon

Analysis of Trichosporon Isolates Obtained from the Houses of Patients with Summer-Type Hypersensitivity Pneumonitis

First Report of Trichosporon ovoides Isolated from the Home of a Summer‐Type Hypersensitivity Pneumonitis Patient

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