Serological cross-reactivity between polysaccharide A and teichoic acid of Staphylococcus aureus

Haukenes, Gunnar; Ellwood, D. C.; Baddiley, J.; Oeding, Per

doi:10.1016/0006-3002(61)90461-9

Cited by 75 publications

(25 citation statements)

References 5 publications

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“…Interestingly, however, enzyme preparations from several different strains appear capable of achieving α-and β-O-GlcNAcylation of WTA to various extents, regardless of the strains tendency to do so natively (24). Furthermore, it has been reported that WTA from S. aureus strain H displays varied content of α-O-linked GlcNAc upon slight variations in its preparation (43). These observations together would suggest underlying regulation in the expression or activity of the WTA GTs.…”

Section: Discussionmentioning

confidence: 89%

Structure and mechanism ofStaphylococcus aureusTarM, the wall teichoic acid α-glycosyltransferase

Sobhanifar

Worrall

Gruninger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Unique to Gram-positive bacteria, wall teichoic acids are anionic glycopolymers cross-stitched to a thick layer of peptidoglycan. The polyol phosphate subunits of these glycopolymers are decorated with GlcNAc sugars that are involved in phage binding, genetic exchange, host antibody response, resistance, and virulence. The search for the enzymes responsible for GlcNAcylation in Staphylococcus aureus has recently identified TarM and TarS with respective α-and β-(1-4) glycosyltransferase activities. The stereochemistry of the GlcNAc attachment is important in balancing biological processes, such that the interplay of TarM and TarS is likely important for bacterial pathogenicity and survival. Here we present the crystal structure of TarM in an unusual ternary-like complex consisting of a polymeric acceptor substrate analog, UDP from a hydrolyzed donor, and an α-glyceryl-GlcNAc product formed in situ. These structures support an internal nucleophilic substitution-like mechanism, lend new mechanistic insight into the glycosylation of glycopolymers, and reveal a trimerization domain with a likely role in acceptor substrate scaffolding.glycosyltransferase | wall teichoic acid | cell wall | crystal structure | bacterial pathogenicity

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Section: Discussionmentioning

confidence: 89%

Structure and mechanism ofStaphylococcus aureusTarM, the wall teichoic acid α-glycosyltransferase

Sobhanifar

Worrall

Gruninger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…The first four amino acids have been found by several investigators in the cell wall mucopeptide of S. aureus in combination with amino sugars, one of which is invariably muramic acid (34). Our chemical analysis of A-3 and its 'identity reaction' with polysaccharide A emphasized that A-3 contained a mucopeptide moiety and probably also teichoic acid (1,29,6).…”

Section: Analysis Of Fractions A-3 A-2 B and Cmentioning

confidence: 83%

Immunochemical Analysis of Staphylococcal Antigenic Preparations

Löfkvist¹,

Sjöquist²

1964

Int Arch Allergy Immunol

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“…indicated that their presence within the cell wall of an organism was of taxonomic significance and could be correlated with serological behaviour (Baddiley & Davison, 1961 ;Davison & Baddiley, 1963). In staphylococci, ribitol teichoic acid containing /l-glucosaminyl residues from walls of Staphylococcus aureus (Baddiley, Buchanan, RajBhandary & Sanderson, 1962 a ; Baddiley, Buchanan, Martin & RajBhandary, 1962 b) is serologically indistinguishable from the group-specific precipitinogen, polysaccharide A, of this organism (Haukenes, Ellwood, Baddiley & Oeding, 1961; Haukenes, 1962), and glycerol teichoic acid containing glucosyl residues from walls of S . saprophyticus (Davison & Baddiley, 1963) is the group-specific precipitinogen, polysaccharide B, of S. albus (Morse, 1963).…”

Section: Introductionmentioning

confidence: 97%

Teichoic Acids and Group Antigens in Lactobacilli

Me¹,

Baddiley

1964

Journal of General Microbiology

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SUMMARYPurified teichoic acids and extracts with group-specific activity have been examined with specific antisera for a number of lactobacilli. The wall teichoic acids from groups D and E lactobacilli have been identified as the group-specific substances, whereas in group A the intracellular teichoic acid showed group-specificity. It is possible that group-specificity is associated with intracellular teichoic acid in group F.

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Serological cross-reactivity between polysaccharide A and teichoic acid of Staphylococcus aureus

Cited by 75 publications

References 5 publications

Structure and mechanism ofStaphylococcus aureusTarM, the wall teichoic acid α-glycosyltransferase

Structure and mechanism ofStaphylococcus aureusTarM, the wall teichoic acid α-glycosyltransferase

Immunochemical Analysis of Staphylococcal Antigenic Preparations

Teichoic Acids and Group Antigens in Lactobacilli

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